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PKC regulates the production of fibroblast growth factor 23 (FGF23)
Serine/threonine protein kinase C (PKC) is activated by diacylglycerol that is released from membrane lipids by phospholipase C in response to activation of G protein-coupled receptors or receptor tyrosine kinases. PKC isoforms are particularly relevant for proliferation and differentiation of cells...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438472/ https://www.ncbi.nlm.nih.gov/pubmed/30921339 http://dx.doi.org/10.1371/journal.pone.0211309 |
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author | Bär, Ludmilla Hase, Philipp Föller, Michael |
author_facet | Bär, Ludmilla Hase, Philipp Föller, Michael |
author_sort | Bär, Ludmilla |
collection | PubMed |
description | Serine/threonine protein kinase C (PKC) is activated by diacylglycerol that is released from membrane lipids by phospholipase C in response to activation of G protein-coupled receptors or receptor tyrosine kinases. PKC isoforms are particularly relevant for proliferation and differentiation of cells including osteoblasts. Osteoblasts/osteocytes produce fibroblast growth factor 23 (FGF23), a hormone regulating renal phosphate and vitamin D handling. PKC activates NFκB, a transcription factor complex controlling FGF23 expression. Here, we analyzed the impact of PKC on FGF23 synthesis. Fgf23 expression was analyzed by qRT-PCR in UMR106 osteoblast-like cells and in IDG-SW3 osteocytes, and FGF23 protein was measured by ELISA. Phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA), a PKC activator, up-regulated FGF23 production. In contrast, PKC inhibitors calphostin C, Gö6976, sotrastaurin and ruboxistaurin suppressed FGF23 formation. NFκB inhibitor withaferin A abolished the stimulatory effect of PMA on Fgf23. PKC is a powerful regulator of FGF23 synthesis, an effect which is at least partly mediated by NFκB. |
format | Online Article Text |
id | pubmed-6438472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64384722019-04-12 PKC regulates the production of fibroblast growth factor 23 (FGF23) Bär, Ludmilla Hase, Philipp Föller, Michael PLoS One Research Article Serine/threonine protein kinase C (PKC) is activated by diacylglycerol that is released from membrane lipids by phospholipase C in response to activation of G protein-coupled receptors or receptor tyrosine kinases. PKC isoforms are particularly relevant for proliferation and differentiation of cells including osteoblasts. Osteoblasts/osteocytes produce fibroblast growth factor 23 (FGF23), a hormone regulating renal phosphate and vitamin D handling. PKC activates NFκB, a transcription factor complex controlling FGF23 expression. Here, we analyzed the impact of PKC on FGF23 synthesis. Fgf23 expression was analyzed by qRT-PCR in UMR106 osteoblast-like cells and in IDG-SW3 osteocytes, and FGF23 protein was measured by ELISA. Phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA), a PKC activator, up-regulated FGF23 production. In contrast, PKC inhibitors calphostin C, Gö6976, sotrastaurin and ruboxistaurin suppressed FGF23 formation. NFκB inhibitor withaferin A abolished the stimulatory effect of PMA on Fgf23. PKC is a powerful regulator of FGF23 synthesis, an effect which is at least partly mediated by NFκB. Public Library of Science 2019-03-28 /pmc/articles/PMC6438472/ /pubmed/30921339 http://dx.doi.org/10.1371/journal.pone.0211309 Text en © 2019 Bär et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bär, Ludmilla Hase, Philipp Föller, Michael PKC regulates the production of fibroblast growth factor 23 (FGF23) |
title | PKC regulates the production of fibroblast growth factor 23 (FGF23) |
title_full | PKC regulates the production of fibroblast growth factor 23 (FGF23) |
title_fullStr | PKC regulates the production of fibroblast growth factor 23 (FGF23) |
title_full_unstemmed | PKC regulates the production of fibroblast growth factor 23 (FGF23) |
title_short | PKC regulates the production of fibroblast growth factor 23 (FGF23) |
title_sort | pkc regulates the production of fibroblast growth factor 23 (fgf23) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438472/ https://www.ncbi.nlm.nih.gov/pubmed/30921339 http://dx.doi.org/10.1371/journal.pone.0211309 |
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