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Copy number variation study in Japanese quail associated with stress related traits using whole genome re-sequencing data

Copy number variation (CNV) is a major driving factor for genetic variation and phenotypic diversity in animals. To detect CNVs and understand genetic components underlying stress related traits, we performed whole genome re-sequencing of pooled DNA samples of 20 birds each from High Stress (HS) and...

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Autores principales: Khatri, Bhuwan, Kang, Seong, Shouse, Stephanie, Anthony, Nicholas, Kuenzel, Wayne, Kong, Byungwhi C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438477/
https://www.ncbi.nlm.nih.gov/pubmed/30921419
http://dx.doi.org/10.1371/journal.pone.0214543
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author Khatri, Bhuwan
Kang, Seong
Shouse, Stephanie
Anthony, Nicholas
Kuenzel, Wayne
Kong, Byungwhi C.
author_facet Khatri, Bhuwan
Kang, Seong
Shouse, Stephanie
Anthony, Nicholas
Kuenzel, Wayne
Kong, Byungwhi C.
author_sort Khatri, Bhuwan
collection PubMed
description Copy number variation (CNV) is a major driving factor for genetic variation and phenotypic diversity in animals. To detect CNVs and understand genetic components underlying stress related traits, we performed whole genome re-sequencing of pooled DNA samples of 20 birds each from High Stress (HS) and Low Stress (LS) Japanese quail lines using Illumina HiSeq 2×150 bp paired end method. Sequencing data were aligned to the quail genome and CNVnator was used to detect CNVs in the aligned data sets. The depth of coverage for the data reached to 41.4x and 42.6x for HS and LS birds, respectively. We identified 262 and 168 CNV regions affecting 1.6 and 1.9% of the reference genome that completely overlapped 454 and 493 unique genes in HS and LS birds, respectively. Ingenuity pathway analysis showed that the CNV genes were significantly enriched to phospholipase C signaling, neuregulin signaling, reelin signaling in neurons, endocrine and nervous development, humoral immune response, and carbohydrate and amino acid metabolisms in HS birds, whereas CNV genes in LS birds were enriched in cell-mediated immune response, and protein and lipid metabolisms. These findings suggest CNV genes identified in HS and LS birds could be candidate markers responsible for stress responses in birds.
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spelling pubmed-64384772019-04-12 Copy number variation study in Japanese quail associated with stress related traits using whole genome re-sequencing data Khatri, Bhuwan Kang, Seong Shouse, Stephanie Anthony, Nicholas Kuenzel, Wayne Kong, Byungwhi C. PLoS One Research Article Copy number variation (CNV) is a major driving factor for genetic variation and phenotypic diversity in animals. To detect CNVs and understand genetic components underlying stress related traits, we performed whole genome re-sequencing of pooled DNA samples of 20 birds each from High Stress (HS) and Low Stress (LS) Japanese quail lines using Illumina HiSeq 2×150 bp paired end method. Sequencing data were aligned to the quail genome and CNVnator was used to detect CNVs in the aligned data sets. The depth of coverage for the data reached to 41.4x and 42.6x for HS and LS birds, respectively. We identified 262 and 168 CNV regions affecting 1.6 and 1.9% of the reference genome that completely overlapped 454 and 493 unique genes in HS and LS birds, respectively. Ingenuity pathway analysis showed that the CNV genes were significantly enriched to phospholipase C signaling, neuregulin signaling, reelin signaling in neurons, endocrine and nervous development, humoral immune response, and carbohydrate and amino acid metabolisms in HS birds, whereas CNV genes in LS birds were enriched in cell-mediated immune response, and protein and lipid metabolisms. These findings suggest CNV genes identified in HS and LS birds could be candidate markers responsible for stress responses in birds. Public Library of Science 2019-03-28 /pmc/articles/PMC6438477/ /pubmed/30921419 http://dx.doi.org/10.1371/journal.pone.0214543 Text en © 2019 Khatri et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Khatri, Bhuwan
Kang, Seong
Shouse, Stephanie
Anthony, Nicholas
Kuenzel, Wayne
Kong, Byungwhi C.
Copy number variation study in Japanese quail associated with stress related traits using whole genome re-sequencing data
title Copy number variation study in Japanese quail associated with stress related traits using whole genome re-sequencing data
title_full Copy number variation study in Japanese quail associated with stress related traits using whole genome re-sequencing data
title_fullStr Copy number variation study in Japanese quail associated with stress related traits using whole genome re-sequencing data
title_full_unstemmed Copy number variation study in Japanese quail associated with stress related traits using whole genome re-sequencing data
title_short Copy number variation study in Japanese quail associated with stress related traits using whole genome re-sequencing data
title_sort copy number variation study in japanese quail associated with stress related traits using whole genome re-sequencing data
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438477/
https://www.ncbi.nlm.nih.gov/pubmed/30921419
http://dx.doi.org/10.1371/journal.pone.0214543
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