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IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging

Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless...

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Detalles Bibliográficos
Autor principal: Dormann, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438524/
https://www.ncbi.nlm.nih.gov/pubmed/30921439
http://dx.doi.org/10.1371/journal.pone.0214659
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author Dormann, Dirk
author_facet Dormann, Dirk
author_sort Dormann, Dirk
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description Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance.
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spelling pubmed-64385242019-04-12 IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging Dormann, Dirk PLoS One Research Article Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance. Public Library of Science 2019-03-28 /pmc/articles/PMC6438524/ /pubmed/30921439 http://dx.doi.org/10.1371/journal.pone.0214659 Text en © 2019 Dirk Dormann http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dormann, Dirk
IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging
title IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging
title_full IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging
title_fullStr IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging
title_full_unstemmed IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging
title_short IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging
title_sort intensitycheck – the light measuring app for microscope performance checks and consistent fluorescence imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438524/
https://www.ncbi.nlm.nih.gov/pubmed/30921439
http://dx.doi.org/10.1371/journal.pone.0214659
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