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IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging
Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438524/ https://www.ncbi.nlm.nih.gov/pubmed/30921439 http://dx.doi.org/10.1371/journal.pone.0214659 |
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author | Dormann, Dirk |
author_facet | Dormann, Dirk |
author_sort | Dormann, Dirk |
collection | PubMed |
description | Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance. |
format | Online Article Text |
id | pubmed-6438524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64385242019-04-12 IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging Dormann, Dirk PLoS One Research Article Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance. Public Library of Science 2019-03-28 /pmc/articles/PMC6438524/ /pubmed/30921439 http://dx.doi.org/10.1371/journal.pone.0214659 Text en © 2019 Dirk Dormann http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dormann, Dirk IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging |
title | IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging |
title_full | IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging |
title_fullStr | IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging |
title_full_unstemmed | IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging |
title_short | IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging |
title_sort | intensitycheck – the light measuring app for microscope performance checks and consistent fluorescence imaging |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438524/ https://www.ncbi.nlm.nih.gov/pubmed/30921439 http://dx.doi.org/10.1371/journal.pone.0214659 |
work_keys_str_mv | AT dormanndirk intensitycheckthelightmeasuringappformicroscopeperformancechecksandconsistentfluorescenceimaging |