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Tokiinshi, a traditional Japanese medicine (Kampo), suppresses Panton-Valentine leukocidin production in the methicillin-resistant Staphylococcus aureus USA300 clone

It is necessary to develop agents other than antimicrobials for the treatment of Staphylococcus aureus infections to prevent the emergence of antimicrobial-resistant strains. Particularly, anti-virulence agents against the Panton-Valentine leukocidin (PVL)-positive methicillin-resistant S. aureus (M...

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Autores principales: Maezawa, Yuka, Nakaminami, Hidemasa, Takadama, Shunsuke, Hayashi, Minami, Wajima, Takeaki, Nakase, Keisuke, Yamada, Tetsuya, Ikoshi, Hideaki, Noguchi, Norihisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438529/
https://www.ncbi.nlm.nih.gov/pubmed/30921402
http://dx.doi.org/10.1371/journal.pone.0214470
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author Maezawa, Yuka
Nakaminami, Hidemasa
Takadama, Shunsuke
Hayashi, Minami
Wajima, Takeaki
Nakase, Keisuke
Yamada, Tetsuya
Ikoshi, Hideaki
Noguchi, Norihisa
author_facet Maezawa, Yuka
Nakaminami, Hidemasa
Takadama, Shunsuke
Hayashi, Minami
Wajima, Takeaki
Nakase, Keisuke
Yamada, Tetsuya
Ikoshi, Hideaki
Noguchi, Norihisa
author_sort Maezawa, Yuka
collection PubMed
description It is necessary to develop agents other than antimicrobials for the treatment of Staphylococcus aureus infections to prevent the emergence of antimicrobial-resistant strains. Particularly, anti-virulence agents against the Panton-Valentine leukocidin (PVL)-positive methicillin-resistant S. aureus (MRSA), USA300 clone, is desired due to its high pathogenicity. Here, we investigated the potential anti-virulence effect of Tokiinshi, which is a traditional Japanese medicine (Kampo) used for skin diseases, against the USA300 clone. A growth inhibition assay showed that a conventional dose (20 mg/ml) of Tokiinshi has bactericidal effects against the clinical USA300 clones. Notably, the growth inhibition effects of Tokiinshi against S. epidermidis strains, which are the major constituents of the skin microbiome, was a bacteriostatic effect. The data suggested that Tokiinshi is unlikely to affect skin flora of S. epidermidis. Furthermore, PVL production and the expression of its gene were significantly suppressed in the USA300 clone by a lower concentration (5 mg/ml) of Tokiinshi. This did not affect the number of viable bacteria. Moreover, Tokiinshi significantly suppressed the expression of the agrA gene, which regulates PVL gene expression. For the first time, our findings strongly suggest that Tokiinshi has the potential to attenuate the virulence of the USA300 clone by suppressing PVL production via agrA gene suppression.
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spelling pubmed-64385292019-04-12 Tokiinshi, a traditional Japanese medicine (Kampo), suppresses Panton-Valentine leukocidin production in the methicillin-resistant Staphylococcus aureus USA300 clone Maezawa, Yuka Nakaminami, Hidemasa Takadama, Shunsuke Hayashi, Minami Wajima, Takeaki Nakase, Keisuke Yamada, Tetsuya Ikoshi, Hideaki Noguchi, Norihisa PLoS One Research Article It is necessary to develop agents other than antimicrobials for the treatment of Staphylococcus aureus infections to prevent the emergence of antimicrobial-resistant strains. Particularly, anti-virulence agents against the Panton-Valentine leukocidin (PVL)-positive methicillin-resistant S. aureus (MRSA), USA300 clone, is desired due to its high pathogenicity. Here, we investigated the potential anti-virulence effect of Tokiinshi, which is a traditional Japanese medicine (Kampo) used for skin diseases, against the USA300 clone. A growth inhibition assay showed that a conventional dose (20 mg/ml) of Tokiinshi has bactericidal effects against the clinical USA300 clones. Notably, the growth inhibition effects of Tokiinshi against S. epidermidis strains, which are the major constituents of the skin microbiome, was a bacteriostatic effect. The data suggested that Tokiinshi is unlikely to affect skin flora of S. epidermidis. Furthermore, PVL production and the expression of its gene were significantly suppressed in the USA300 clone by a lower concentration (5 mg/ml) of Tokiinshi. This did not affect the number of viable bacteria. Moreover, Tokiinshi significantly suppressed the expression of the agrA gene, which regulates PVL gene expression. For the first time, our findings strongly suggest that Tokiinshi has the potential to attenuate the virulence of the USA300 clone by suppressing PVL production via agrA gene suppression. Public Library of Science 2019-03-28 /pmc/articles/PMC6438529/ /pubmed/30921402 http://dx.doi.org/10.1371/journal.pone.0214470 Text en © 2019 Maezawa et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Maezawa, Yuka
Nakaminami, Hidemasa
Takadama, Shunsuke
Hayashi, Minami
Wajima, Takeaki
Nakase, Keisuke
Yamada, Tetsuya
Ikoshi, Hideaki
Noguchi, Norihisa
Tokiinshi, a traditional Japanese medicine (Kampo), suppresses Panton-Valentine leukocidin production in the methicillin-resistant Staphylococcus aureus USA300 clone
title Tokiinshi, a traditional Japanese medicine (Kampo), suppresses Panton-Valentine leukocidin production in the methicillin-resistant Staphylococcus aureus USA300 clone
title_full Tokiinshi, a traditional Japanese medicine (Kampo), suppresses Panton-Valentine leukocidin production in the methicillin-resistant Staphylococcus aureus USA300 clone
title_fullStr Tokiinshi, a traditional Japanese medicine (Kampo), suppresses Panton-Valentine leukocidin production in the methicillin-resistant Staphylococcus aureus USA300 clone
title_full_unstemmed Tokiinshi, a traditional Japanese medicine (Kampo), suppresses Panton-Valentine leukocidin production in the methicillin-resistant Staphylococcus aureus USA300 clone
title_short Tokiinshi, a traditional Japanese medicine (Kampo), suppresses Panton-Valentine leukocidin production in the methicillin-resistant Staphylococcus aureus USA300 clone
title_sort tokiinshi, a traditional japanese medicine (kampo), suppresses panton-valentine leukocidin production in the methicillin-resistant staphylococcus aureus usa300 clone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438529/
https://www.ncbi.nlm.nih.gov/pubmed/30921402
http://dx.doi.org/10.1371/journal.pone.0214470
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