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Evaluation of biologically synthesized Au-CuO and CuO-ZnO nanoparticles against glioma cells and microorganisms

Due to the search for new methods of producing bimetallic nanoparticles, in this work, we have conducted a biological synthesis of Au-CuO and CuO-ZnO nanoparticles using Cnici benedicti. The synthesized Au-CuO and CuO-ZnO nanoparticles were also analyzed in terms of their antibacterial activity, as...

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Autores principales: Dobrucka, Renata, Kaczmarek, Mariusz, Łagiedo, Małgorzata, Kielan, Agata, Dlugaszewska, Jolanta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438707/
https://www.ncbi.nlm.nih.gov/pubmed/30976181
http://dx.doi.org/10.1016/j.jsps.2018.12.006
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author Dobrucka, Renata
Kaczmarek, Mariusz
Łagiedo, Małgorzata
Kielan, Agata
Dlugaszewska, Jolanta
author_facet Dobrucka, Renata
Kaczmarek, Mariusz
Łagiedo, Małgorzata
Kielan, Agata
Dlugaszewska, Jolanta
author_sort Dobrucka, Renata
collection PubMed
description Due to the search for new methods of producing bimetallic nanoparticles, in this work, we have conducted a biological synthesis of Au-CuO and CuO-ZnO nanoparticles using Cnici benedicti. The synthesized Au-CuO and CuO-ZnO nanoparticles were also analyzed in terms of their antibacterial activity, as well as their influence on cell viability, using two specific cell lines: C6 rat brain glioma (ATCC® CCL-107™) and T98G human glioma (ATCC® CRL-1690™). The studies carried out by means of Atomic Force Microscopy helped to determine the presence Au-CuO nanoparticles whose size was about 13 nm. The size of CuO-ZnO nanoparticles was about 28 nm. The obtained nanoparticles showed cidal activity against glioma cells depending on the concentration of the substance and the time of culture. In the first stage, the nanoparticles limited the ability to divide cells; then, they blocked the cell cycle in the G2 – M phase, and finally led to massive cell death. The antimicrobial activity studies showed that Au-CuO nanoparticles inhibited the growth of microorganisms at lower concentrations than CuO-ZnO nanoparticles, and both kinds of nanoparticles showed excellent cidal properties.
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spelling pubmed-64387072019-04-11 Evaluation of biologically synthesized Au-CuO and CuO-ZnO nanoparticles against glioma cells and microorganisms Dobrucka, Renata Kaczmarek, Mariusz Łagiedo, Małgorzata Kielan, Agata Dlugaszewska, Jolanta Saudi Pharm J Article Due to the search for new methods of producing bimetallic nanoparticles, in this work, we have conducted a biological synthesis of Au-CuO and CuO-ZnO nanoparticles using Cnici benedicti. The synthesized Au-CuO and CuO-ZnO nanoparticles were also analyzed in terms of their antibacterial activity, as well as their influence on cell viability, using two specific cell lines: C6 rat brain glioma (ATCC® CCL-107™) and T98G human glioma (ATCC® CRL-1690™). The studies carried out by means of Atomic Force Microscopy helped to determine the presence Au-CuO nanoparticles whose size was about 13 nm. The size of CuO-ZnO nanoparticles was about 28 nm. The obtained nanoparticles showed cidal activity against glioma cells depending on the concentration of the substance and the time of culture. In the first stage, the nanoparticles limited the ability to divide cells; then, they blocked the cell cycle in the G2 – M phase, and finally led to massive cell death. The antimicrobial activity studies showed that Au-CuO nanoparticles inhibited the growth of microorganisms at lower concentrations than CuO-ZnO nanoparticles, and both kinds of nanoparticles showed excellent cidal properties. Elsevier 2019-03 2018-12-17 /pmc/articles/PMC6438707/ /pubmed/30976181 http://dx.doi.org/10.1016/j.jsps.2018.12.006 Text en © 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Dobrucka, Renata
Kaczmarek, Mariusz
Łagiedo, Małgorzata
Kielan, Agata
Dlugaszewska, Jolanta
Evaluation of biologically synthesized Au-CuO and CuO-ZnO nanoparticles against glioma cells and microorganisms
title Evaluation of biologically synthesized Au-CuO and CuO-ZnO nanoparticles against glioma cells and microorganisms
title_full Evaluation of biologically synthesized Au-CuO and CuO-ZnO nanoparticles against glioma cells and microorganisms
title_fullStr Evaluation of biologically synthesized Au-CuO and CuO-ZnO nanoparticles against glioma cells and microorganisms
title_full_unstemmed Evaluation of biologically synthesized Au-CuO and CuO-ZnO nanoparticles against glioma cells and microorganisms
title_short Evaluation of biologically synthesized Au-CuO and CuO-ZnO nanoparticles against glioma cells and microorganisms
title_sort evaluation of biologically synthesized au-cuo and cuo-zno nanoparticles against glioma cells and microorganisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438707/
https://www.ncbi.nlm.nih.gov/pubmed/30976181
http://dx.doi.org/10.1016/j.jsps.2018.12.006
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