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Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study

Diabetes mellitus (DM) has emerged as a public healthcare problem. Sustained hyperglycemia has been linked with many complications including impaired male fertility Olive tree (Olea europaea L.) leaves have been extensively used in traditional remedies worldwide to control blood glucose level in DM....

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Autores principales: Soliman, Gamal A., Saeedan, Abdulaziz S., Abdel-Rahman, Rehab F., Ogaly, Hanan A., Abd-Elsalam, Reham M., Abdel-Kader, Maged S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438850/
https://www.ncbi.nlm.nih.gov/pubmed/30976175
http://dx.doi.org/10.1016/j.jsps.2018.11.015
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author Soliman, Gamal A.
Saeedan, Abdulaziz S.
Abdel-Rahman, Rehab F.
Ogaly, Hanan A.
Abd-Elsalam, Reham M.
Abdel-Kader, Maged S.
author_facet Soliman, Gamal A.
Saeedan, Abdulaziz S.
Abdel-Rahman, Rehab F.
Ogaly, Hanan A.
Abd-Elsalam, Reham M.
Abdel-Kader, Maged S.
author_sort Soliman, Gamal A.
collection PubMed
description Diabetes mellitus (DM) has emerged as a public healthcare problem. Sustained hyperglycemia has been linked with many complications including impaired male fertility Olive tree (Olea europaea L.) leaves have been extensively used in traditional remedies worldwide to control blood glucose level in DM. In this study, the beneficial role of olive leaves extract (OLE) was investigated to combat diabetes-induced adverse effect on testicular tissues. Thirty male Wistar rats were divided into 5 equal groups: normal control group, streptozotocin (STZ)-diabetic group and diabetic groups which were given glibenclamide (GLB) or OLE at 250 and 500 mg/kg for 9 weeks to investigate the efficiency of olive leaves extract (OLE) in reducing the deleterious effect of diabetes on the reproductive system of male rats. Rats were checked for serum glucose, insulin, testosterone and gonadotropins. Also, testicular antioxidants, epididymal sperm characteristics and testicular histopathology were assessed. Expression of the testicular steroidogenic enzymes, cholesterol side-chain cleavage enzyme (P450 scc) and 17β-hydroxysteroid dehydrogenase (17β-HSD) was examined. Moreover, androgen receptor and proliferating cell nuclear antigen (PCNA) protein immunohistochemistry were assessed in testes. STZ-induced diabetes significantly increased serum glucose. However, STZ significantly decreased serum levels of insulin, testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH). Marked reductions in testicular antioxidants with elevated malondialdehyde (MDA) parallel with deterioration of the testicular histoarchitecture and epididymal sperm characteristics were recorded. Administration of GLB or OLE (250 and 500 mg/kg) resulted in a significant recovery of the above mentioned parameters in STZ-diabetic rats. Interestingly, OLE shows greater glycemic improvement and testicular protection than GLB with the highest percentage protection exhibited by the OLE high dose. Furthermore, OLE significantly induced testicular steroidogenesis in diabetic rat as evidenced by elevated P450 scc and 17β-HSD mRNA expression. The study proves that OLE possesses a potential protective role against diabetes-induced reproductive disorders, which may be due to its antioxidant activity and its ability to normalize testicular steroidogenesis.
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spelling pubmed-64388502019-04-11 Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study Soliman, Gamal A. Saeedan, Abdulaziz S. Abdel-Rahman, Rehab F. Ogaly, Hanan A. Abd-Elsalam, Reham M. Abdel-Kader, Maged S. Saudi Pharm J Article Diabetes mellitus (DM) has emerged as a public healthcare problem. Sustained hyperglycemia has been linked with many complications including impaired male fertility Olive tree (Olea europaea L.) leaves have been extensively used in traditional remedies worldwide to control blood glucose level in DM. In this study, the beneficial role of olive leaves extract (OLE) was investigated to combat diabetes-induced adverse effect on testicular tissues. Thirty male Wistar rats were divided into 5 equal groups: normal control group, streptozotocin (STZ)-diabetic group and diabetic groups which were given glibenclamide (GLB) or OLE at 250 and 500 mg/kg for 9 weeks to investigate the efficiency of olive leaves extract (OLE) in reducing the deleterious effect of diabetes on the reproductive system of male rats. Rats were checked for serum glucose, insulin, testosterone and gonadotropins. Also, testicular antioxidants, epididymal sperm characteristics and testicular histopathology were assessed. Expression of the testicular steroidogenic enzymes, cholesterol side-chain cleavage enzyme (P450 scc) and 17β-hydroxysteroid dehydrogenase (17β-HSD) was examined. Moreover, androgen receptor and proliferating cell nuclear antigen (PCNA) protein immunohistochemistry were assessed in testes. STZ-induced diabetes significantly increased serum glucose. However, STZ significantly decreased serum levels of insulin, testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH). Marked reductions in testicular antioxidants with elevated malondialdehyde (MDA) parallel with deterioration of the testicular histoarchitecture and epididymal sperm characteristics were recorded. Administration of GLB or OLE (250 and 500 mg/kg) resulted in a significant recovery of the above mentioned parameters in STZ-diabetic rats. Interestingly, OLE shows greater glycemic improvement and testicular protection than GLB with the highest percentage protection exhibited by the OLE high dose. Furthermore, OLE significantly induced testicular steroidogenesis in diabetic rat as evidenced by elevated P450 scc and 17β-HSD mRNA expression. The study proves that OLE possesses a potential protective role against diabetes-induced reproductive disorders, which may be due to its antioxidant activity and its ability to normalize testicular steroidogenesis. Elsevier 2019-03 2018-11-29 /pmc/articles/PMC6438850/ /pubmed/30976175 http://dx.doi.org/10.1016/j.jsps.2018.11.015 Text en © 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Soliman, Gamal A.
Saeedan, Abdulaziz S.
Abdel-Rahman, Rehab F.
Ogaly, Hanan A.
Abd-Elsalam, Reham M.
Abdel-Kader, Maged S.
Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study
title Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study
title_full Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study
title_fullStr Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study
title_full_unstemmed Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study
title_short Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study
title_sort olive leaves extract attenuates type ii diabetes mellitus-induced testicular damage in rats: molecular and biochemical study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438850/
https://www.ncbi.nlm.nih.gov/pubmed/30976175
http://dx.doi.org/10.1016/j.jsps.2018.11.015
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