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The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats

Metabolic surgery ameliorates insulin resistance and is associated with long-term, effective weight loss, but the mechanisms involved remain unknown. Here, the duodenal-jejunal omega switch (DJOS) surgery in combination with high-fat, high-carbohydrate diet was performed on diet obese rats and joint...

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Autores principales: Stygar, Dominika, Skrzep-Poloczek, Bronisława, Romuk, Ewa, Chełmecka, Elżbieta, Poloczek, Jakub, Sawczyn, Tomasz, Maciarz, Justyna, Kukla, Michał, Karcz, Konrad W., Jochem, Jerzy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439084/
https://www.ncbi.nlm.nih.gov/pubmed/30840227
http://dx.doi.org/10.1007/s12192-019-00976-2
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author Stygar, Dominika
Skrzep-Poloczek, Bronisława
Romuk, Ewa
Chełmecka, Elżbieta
Poloczek, Jakub
Sawczyn, Tomasz
Maciarz, Justyna
Kukla, Michał
Karcz, Konrad W.
Jochem, Jerzy
author_facet Stygar, Dominika
Skrzep-Poloczek, Bronisława
Romuk, Ewa
Chełmecka, Elżbieta
Poloczek, Jakub
Sawczyn, Tomasz
Maciarz, Justyna
Kukla, Michał
Karcz, Konrad W.
Jochem, Jerzy
author_sort Stygar, Dominika
collection PubMed
description Metabolic surgery ameliorates insulin resistance and is associated with long-term, effective weight loss, but the mechanisms involved remain unknown. Here, the duodenal-jejunal omega switch (DJOS) surgery in combination with high-fat, high-carbohydrate diet was performed on diet obese rats and joint effects of bariatric surgery and different dietary patterns on heat shock protein 70 (HSP70) and HSP90 plasma and liver concentrations were measured. We found that plasma and liver levels of HSP70 were lower after DJOS surgery in comparison to the control in the groups of animals kept on control diet (CD) and high-fat, high-sugar diet (HFS) but the postoperative change of the diet led to the increase in HSP70 in plasma and liver concentration in DJOS-operated animals. A high-calorie meal, rich in carbohydrates and fats, significantly increased circulating levels of HSP90, reducing the normalising effect of DJOS. The HFS diet applied during all stages of the experiment led to the higher levels of liver HSP90 concentration. The combination of CD and DJOS surgery was the most efficient in the lowering of the HSP90 liver concentration. The normalisation of circulating levels and liver concentrations of HSP70 and HSP90 may be achieved in a combination of DJOS procedure with a proper dietary plan.
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spelling pubmed-64390842019-04-15 The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats Stygar, Dominika Skrzep-Poloczek, Bronisława Romuk, Ewa Chełmecka, Elżbieta Poloczek, Jakub Sawczyn, Tomasz Maciarz, Justyna Kukla, Michał Karcz, Konrad W. Jochem, Jerzy Cell Stress Chaperones Original Paper Metabolic surgery ameliorates insulin resistance and is associated with long-term, effective weight loss, but the mechanisms involved remain unknown. Here, the duodenal-jejunal omega switch (DJOS) surgery in combination with high-fat, high-carbohydrate diet was performed on diet obese rats and joint effects of bariatric surgery and different dietary patterns on heat shock protein 70 (HSP70) and HSP90 plasma and liver concentrations were measured. We found that plasma and liver levels of HSP70 were lower after DJOS surgery in comparison to the control in the groups of animals kept on control diet (CD) and high-fat, high-sugar diet (HFS) but the postoperative change of the diet led to the increase in HSP70 in plasma and liver concentration in DJOS-operated animals. A high-calorie meal, rich in carbohydrates and fats, significantly increased circulating levels of HSP90, reducing the normalising effect of DJOS. The HFS diet applied during all stages of the experiment led to the higher levels of liver HSP90 concentration. The combination of CD and DJOS surgery was the most efficient in the lowering of the HSP90 liver concentration. The normalisation of circulating levels and liver concentrations of HSP70 and HSP90 may be achieved in a combination of DJOS procedure with a proper dietary plan. Springer Netherlands 2019-03-06 2019-03 /pmc/articles/PMC6439084/ /pubmed/30840227 http://dx.doi.org/10.1007/s12192-019-00976-2 Text en © The Author(s) 2019 https://creativecommons.org/licenses/by/4.0/OpenAccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Stygar, Dominika
Skrzep-Poloczek, Bronisława
Romuk, Ewa
Chełmecka, Elżbieta
Poloczek, Jakub
Sawczyn, Tomasz
Maciarz, Justyna
Kukla, Michał
Karcz, Konrad W.
Jochem, Jerzy
The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats
title The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats
title_full The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats
title_fullStr The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats
title_full_unstemmed The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats
title_short The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats
title_sort influence of high-fat, high-sugar diet and bariatric surgery on hsp70 and hsp90 plasma and liver concentrations in diet-induced obese rats
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439084/
https://www.ncbi.nlm.nih.gov/pubmed/30840227
http://dx.doi.org/10.1007/s12192-019-00976-2
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