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Validation of PROTIA™ Allergy-Q 64 Atopy(®) as a Specific IgE Measurement Assay for 10 Major Allergen Components
PURPOSE: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy(®), a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. M...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439187/ https://www.ncbi.nlm.nih.gov/pubmed/30912330 http://dx.doi.org/10.4168/aair.2019.11.3.422 |
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author | Kim, Sung Ryeol Park, Kyung Hee Lee, Jae-Hyun Kim, Bum Joon Hwang, Jae Hwan Lim, Kook Jin Park, Jung-Won |
author_facet | Kim, Sung Ryeol Park, Kyung Hee Lee, Jae-Hyun Kim, Bum Joon Hwang, Jae Hwan Lim, Kook Jin Park, Jung-Won |
author_sort | Kim, Sung Ryeol |
collection | PubMed |
description | PURPOSE: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy(®), a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. METHODS: The sIgE detection by PROTIA™ Allergy-Q 64 Atopy(®) and ImmunoCAP(®) assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA™ Allergy-Q 64 Atopy(®), Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP(®)), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. RESULTS: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). CONCLUSIONS: PROTIA™ Allergy-Q 64 Atopy(®) is reliable and comparable to the ImmunoCAP(®) assay for component-resolved diagnosis. |
format | Online Article Text |
id | pubmed-6439187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease |
record_format | MEDLINE/PubMed |
spelling | pubmed-64391872019-05-01 Validation of PROTIA™ Allergy-Q 64 Atopy(®) as a Specific IgE Measurement Assay for 10 Major Allergen Components Kim, Sung Ryeol Park, Kyung Hee Lee, Jae-Hyun Kim, Bum Joon Hwang, Jae Hwan Lim, Kook Jin Park, Jung-Won Allergy Asthma Immunol Res Original Article PURPOSE: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy(®), a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. METHODS: The sIgE detection by PROTIA™ Allergy-Q 64 Atopy(®) and ImmunoCAP(®) assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA™ Allergy-Q 64 Atopy(®), Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP(®)), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. RESULTS: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). CONCLUSIONS: PROTIA™ Allergy-Q 64 Atopy(®) is reliable and comparable to the ImmunoCAP(®) assay for component-resolved diagnosis. The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2019-03-12 /pmc/articles/PMC6439187/ /pubmed/30912330 http://dx.doi.org/10.4168/aair.2019.11.3.422 Text en Copyright © 2019 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Sung Ryeol Park, Kyung Hee Lee, Jae-Hyun Kim, Bum Joon Hwang, Jae Hwan Lim, Kook Jin Park, Jung-Won Validation of PROTIA™ Allergy-Q 64 Atopy(®) as a Specific IgE Measurement Assay for 10 Major Allergen Components |
title | Validation of PROTIA™ Allergy-Q 64 Atopy(®) as a Specific IgE Measurement Assay for 10 Major Allergen Components |
title_full | Validation of PROTIA™ Allergy-Q 64 Atopy(®) as a Specific IgE Measurement Assay for 10 Major Allergen Components |
title_fullStr | Validation of PROTIA™ Allergy-Q 64 Atopy(®) as a Specific IgE Measurement Assay for 10 Major Allergen Components |
title_full_unstemmed | Validation of PROTIA™ Allergy-Q 64 Atopy(®) as a Specific IgE Measurement Assay for 10 Major Allergen Components |
title_short | Validation of PROTIA™ Allergy-Q 64 Atopy(®) as a Specific IgE Measurement Assay for 10 Major Allergen Components |
title_sort | validation of protia™ allergy-q 64 atopy(®) as a specific ige measurement assay for 10 major allergen components |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439187/ https://www.ncbi.nlm.nih.gov/pubmed/30912330 http://dx.doi.org/10.4168/aair.2019.11.3.422 |
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