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Systems Biology Reveals NR2F6 and TGFB1 as Key Regulators of Feed Efficiency in Beef Cattle

Systems biology approaches are used as strategy to uncover tissue-specific perturbations and regulatory genes related to complex phenotypes. We applied this approach to study feed efficiency (FE) in beef cattle, an important trait both economically and environmentally. Poly-A selected RNA of five ti...

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Autores principales: Alexandre, Pâmela A., Naval-Sanchez, Marina, Porto-Neto, Laercio R., Ferraz, José Bento S., Reverter, Antonio, Fukumasu, Heidge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439317/
https://www.ncbi.nlm.nih.gov/pubmed/30967894
http://dx.doi.org/10.3389/fgene.2019.00230
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author Alexandre, Pâmela A.
Naval-Sanchez, Marina
Porto-Neto, Laercio R.
Ferraz, José Bento S.
Reverter, Antonio
Fukumasu, Heidge
author_facet Alexandre, Pâmela A.
Naval-Sanchez, Marina
Porto-Neto, Laercio R.
Ferraz, José Bento S.
Reverter, Antonio
Fukumasu, Heidge
author_sort Alexandre, Pâmela A.
collection PubMed
description Systems biology approaches are used as strategy to uncover tissue-specific perturbations and regulatory genes related to complex phenotypes. We applied this approach to study feed efficiency (FE) in beef cattle, an important trait both economically and environmentally. Poly-A selected RNA of five tissues (adrenal gland, hypothalamus, liver, skeletal muscle and pituitary) of eighteen young bulls, selected for high and low FE, were sequenced (Illumina HiSeq 2500, 100 bp, pared-end). From the 17,354 expressed genes considering all tissues, 1,335 were prioritized by five selection categories (differentially expressed, harboring SNPs associated with FE, tissue-specific, secreted in plasma and key regulators) and used for network construction. NR2F6 and TGFB1 were identified and validated by motif discovery as key regulators of hepatic inflammatory response and muscle tissue development, respectively, two biological processes demonstrated to be associated with FE. Moreover, we indicated potential biomarkers of FE, which are related to hormonal control of metabolism and sexual maturity. By using robust methodologies and validation strategies, we confirmed the main biological processes related to FE in Bos indicus and indicated candidate genes as regulators or biomarkers of superior animals.
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spelling pubmed-64393172019-04-09 Systems Biology Reveals NR2F6 and TGFB1 as Key Regulators of Feed Efficiency in Beef Cattle Alexandre, Pâmela A. Naval-Sanchez, Marina Porto-Neto, Laercio R. Ferraz, José Bento S. Reverter, Antonio Fukumasu, Heidge Front Genet Genetics Systems biology approaches are used as strategy to uncover tissue-specific perturbations and regulatory genes related to complex phenotypes. We applied this approach to study feed efficiency (FE) in beef cattle, an important trait both economically and environmentally. Poly-A selected RNA of five tissues (adrenal gland, hypothalamus, liver, skeletal muscle and pituitary) of eighteen young bulls, selected for high and low FE, were sequenced (Illumina HiSeq 2500, 100 bp, pared-end). From the 17,354 expressed genes considering all tissues, 1,335 were prioritized by five selection categories (differentially expressed, harboring SNPs associated with FE, tissue-specific, secreted in plasma and key regulators) and used for network construction. NR2F6 and TGFB1 were identified and validated by motif discovery as key regulators of hepatic inflammatory response and muscle tissue development, respectively, two biological processes demonstrated to be associated with FE. Moreover, we indicated potential biomarkers of FE, which are related to hormonal control of metabolism and sexual maturity. By using robust methodologies and validation strategies, we confirmed the main biological processes related to FE in Bos indicus and indicated candidate genes as regulators or biomarkers of superior animals. Frontiers Media S.A. 2019-03-22 /pmc/articles/PMC6439317/ /pubmed/30967894 http://dx.doi.org/10.3389/fgene.2019.00230 Text en Copyright © 2019 Alexandre, Naval-Sanchez, Porto-Neto, Ferraz, Reverter and Fukumasu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Alexandre, Pâmela A.
Naval-Sanchez, Marina
Porto-Neto, Laercio R.
Ferraz, José Bento S.
Reverter, Antonio
Fukumasu, Heidge
Systems Biology Reveals NR2F6 and TGFB1 as Key Regulators of Feed Efficiency in Beef Cattle
title Systems Biology Reveals NR2F6 and TGFB1 as Key Regulators of Feed Efficiency in Beef Cattle
title_full Systems Biology Reveals NR2F6 and TGFB1 as Key Regulators of Feed Efficiency in Beef Cattle
title_fullStr Systems Biology Reveals NR2F6 and TGFB1 as Key Regulators of Feed Efficiency in Beef Cattle
title_full_unstemmed Systems Biology Reveals NR2F6 and TGFB1 as Key Regulators of Feed Efficiency in Beef Cattle
title_short Systems Biology Reveals NR2F6 and TGFB1 as Key Regulators of Feed Efficiency in Beef Cattle
title_sort systems biology reveals nr2f6 and tgfb1 as key regulators of feed efficiency in beef cattle
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439317/
https://www.ncbi.nlm.nih.gov/pubmed/30967894
http://dx.doi.org/10.3389/fgene.2019.00230
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