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A Clinicopathological and Immunofluorescence Study of Intraepidermal Immunobullous Diseases
CONTEXT: Autoimmune blistering diseases (AIBDs) are characterized by autoantibodies directed against antigens of skin. Direct immunofluorescence (DIF) study helps in confirming the diagnosis where histopathology alone is noncontributory. AIMS: This study aimed to evaluate the clinicopathological and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440185/ https://www.ncbi.nlm.nih.gov/pubmed/30983604 http://dx.doi.org/10.4103/ijd.IJD_515_17 |
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author | Basu, Keya Chatterjee, Moumita De, Abhishek Sengupta, Moumita Datta, Chhanda Mitra, Pradip |
author_facet | Basu, Keya Chatterjee, Moumita De, Abhishek Sengupta, Moumita Datta, Chhanda Mitra, Pradip |
author_sort | Basu, Keya |
collection | PubMed |
description | CONTEXT: Autoimmune blistering diseases (AIBDs) are characterized by autoantibodies directed against antigens of skin. Direct immunofluorescence (DIF) study helps in confirming the diagnosis where histopathology alone is noncontributory. AIMS: This study aimed to evaluate the clinicopathological and DIF features of intraepidermal AIBD and to assess their relative diagnostic significance. MATERIALS AND METHODS: It was an institution-based observational study. A total of 34 patients were studied over a period of 1½ years in the Department of Pathology in collaboration with the Department of Dermatology of a tertiary care hospital. The clinical, pathological, and DIF features were evaluated and documented. STATISTICAL ANALYSIS: Data were analyzed by statistical tests using GraphPad InStat. RESULTS: Pemphigus vulgaris (PV) was the predominant type with 18 (53%) cases followed by 15 (44%) cases of pemphigus foliaceus (PF) and a single case of pemphigus erythematosus (PE). The age of the patients ranged from 17 to 85 years. Overall, there was a female preponderance in the study group. The most common presenting feature was pruritus (58.82%). Tzanck smear showed the presence of acantholytic cells in thirty (88.24%) patients. Characteristic histopathological features were present in all the cases of PV and PF except one case of PF which was found to be a case of PE. DIF study showed intraepidermal deposition of intercellular immunoglobulin G (IgG) and C3 both in PV and PF. The case of PE showed epidermal “antinuclear antibody” staining with IgG. CONCLUSION: Immunofluorescence study may be used as an additional tool for confirmation of diagnosis where histopathology alone is inconclusive. |
format | Online Article Text |
id | pubmed-6440185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-64401852019-04-12 A Clinicopathological and Immunofluorescence Study of Intraepidermal Immunobullous Diseases Basu, Keya Chatterjee, Moumita De, Abhishek Sengupta, Moumita Datta, Chhanda Mitra, Pradip Indian J Dermatol Original Article CONTEXT: Autoimmune blistering diseases (AIBDs) are characterized by autoantibodies directed against antigens of skin. Direct immunofluorescence (DIF) study helps in confirming the diagnosis where histopathology alone is noncontributory. AIMS: This study aimed to evaluate the clinicopathological and DIF features of intraepidermal AIBD and to assess their relative diagnostic significance. MATERIALS AND METHODS: It was an institution-based observational study. A total of 34 patients were studied over a period of 1½ years in the Department of Pathology in collaboration with the Department of Dermatology of a tertiary care hospital. The clinical, pathological, and DIF features were evaluated and documented. STATISTICAL ANALYSIS: Data were analyzed by statistical tests using GraphPad InStat. RESULTS: Pemphigus vulgaris (PV) was the predominant type with 18 (53%) cases followed by 15 (44%) cases of pemphigus foliaceus (PF) and a single case of pemphigus erythematosus (PE). The age of the patients ranged from 17 to 85 years. Overall, there was a female preponderance in the study group. The most common presenting feature was pruritus (58.82%). Tzanck smear showed the presence of acantholytic cells in thirty (88.24%) patients. Characteristic histopathological features were present in all the cases of PV and PF except one case of PF which was found to be a case of PE. DIF study showed intraepidermal deposition of intercellular immunoglobulin G (IgG) and C3 both in PV and PF. The case of PE showed epidermal “antinuclear antibody” staining with IgG. CONCLUSION: Immunofluorescence study may be used as an additional tool for confirmation of diagnosis where histopathology alone is inconclusive. Wolters Kluwer - Medknow 2019 /pmc/articles/PMC6440185/ /pubmed/30983604 http://dx.doi.org/10.4103/ijd.IJD_515_17 Text en Copyright: © 2019 Indian Journal of Dermatology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Basu, Keya Chatterjee, Moumita De, Abhishek Sengupta, Moumita Datta, Chhanda Mitra, Pradip A Clinicopathological and Immunofluorescence Study of Intraepidermal Immunobullous Diseases |
title | A Clinicopathological and Immunofluorescence Study of Intraepidermal Immunobullous Diseases |
title_full | A Clinicopathological and Immunofluorescence Study of Intraepidermal Immunobullous Diseases |
title_fullStr | A Clinicopathological and Immunofluorescence Study of Intraepidermal Immunobullous Diseases |
title_full_unstemmed | A Clinicopathological and Immunofluorescence Study of Intraepidermal Immunobullous Diseases |
title_short | A Clinicopathological and Immunofluorescence Study of Intraepidermal Immunobullous Diseases |
title_sort | clinicopathological and immunofluorescence study of intraepidermal immunobullous diseases |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440185/ https://www.ncbi.nlm.nih.gov/pubmed/30983604 http://dx.doi.org/10.4103/ijd.IJD_515_17 |
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