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Differential activity of 16K rat prolactin in different organic systems

The 16K isoform of rat prolactin (16K rPRL) performs multiple functions in various systems including angiogenesis, tumorigenesis, and reproduction. Recently, 16K rPRL has attained prominence as a possible therapeutic target in pathophysiological conditions. However, the integral function and mechani...

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Autores principales: Yun, Bo-Young, Cho, Chunghee, Cho, Byung-Nam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440500/
https://www.ncbi.nlm.nih.gov/pubmed/30949401
http://dx.doi.org/10.1080/19768354.2018.1554543
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author Yun, Bo-Young
Cho, Chunghee
Cho, Byung-Nam
author_facet Yun, Bo-Young
Cho, Chunghee
Cho, Byung-Nam
author_sort Yun, Bo-Young
collection PubMed
description The 16K isoform of rat prolactin (16K rPRL) performs multiple functions in various systems including angiogenesis, tumorigenesis, and reproduction. Recently, 16K rPRL has attained prominence as a possible therapeutic target in pathophysiological conditions. However, the integral function and mechanism of 16K rPRL in various systems has not been elucidated. To this end, a transient gain-of-function animal model was adopted. An expression DNA plasmid containing 16K rPRL or rPRL gene was introduced into the muscle of adult mice by direct injection. The mRNA and protein expression levels of 16K rPRL were detected by initial RT–PCR and subsequent Southern blot and western blot, respectively. When the expression vector was introduced, the results were as follows: First, 16K rPRL combined with rPRL reduced angiogenesis in the testis whereas rPRL alone induced angiogenesis. Second, 16K rPRL combined with rPRL reduced WBC proliferation, whereas rPRL alone increased WBC proliferation. Third, 16K rPRL combined with rPRL reduced diestrus, whereas rPRL alone extended diestrus. Fourth, 16K rPRL combined with rPRL unexpectedly increased testosterone (T) levels, whereas rPRL alone did not increase T levels. Taken together, our data suggest that the 16K rPRL isoform performs integral functions in angiogenesis in the testis, WBC proliferation, and reproduction, although the action of 16K rPRL is not always antagonistic.
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spelling pubmed-64405002019-04-04 Differential activity of 16K rat prolactin in different organic systems Yun, Bo-Young Cho, Chunghee Cho, Byung-Nam Anim Cells Syst (Seoul) Developmental Biology The 16K isoform of rat prolactin (16K rPRL) performs multiple functions in various systems including angiogenesis, tumorigenesis, and reproduction. Recently, 16K rPRL has attained prominence as a possible therapeutic target in pathophysiological conditions. However, the integral function and mechanism of 16K rPRL in various systems has not been elucidated. To this end, a transient gain-of-function animal model was adopted. An expression DNA plasmid containing 16K rPRL or rPRL gene was introduced into the muscle of adult mice by direct injection. The mRNA and protein expression levels of 16K rPRL were detected by initial RT–PCR and subsequent Southern blot and western blot, respectively. When the expression vector was introduced, the results were as follows: First, 16K rPRL combined with rPRL reduced angiogenesis in the testis whereas rPRL alone induced angiogenesis. Second, 16K rPRL combined with rPRL reduced WBC proliferation, whereas rPRL alone increased WBC proliferation. Third, 16K rPRL combined with rPRL reduced diestrus, whereas rPRL alone extended diestrus. Fourth, 16K rPRL combined with rPRL unexpectedly increased testosterone (T) levels, whereas rPRL alone did not increase T levels. Taken together, our data suggest that the 16K rPRL isoform performs integral functions in angiogenesis in the testis, WBC proliferation, and reproduction, although the action of 16K rPRL is not always antagonistic. Taylor & Francis 2019-02-20 /pmc/articles/PMC6440500/ /pubmed/30949401 http://dx.doi.org/10.1080/19768354.2018.1554543 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Developmental Biology
Yun, Bo-Young
Cho, Chunghee
Cho, Byung-Nam
Differential activity of 16K rat prolactin in different organic systems
title Differential activity of 16K rat prolactin in different organic systems
title_full Differential activity of 16K rat prolactin in different organic systems
title_fullStr Differential activity of 16K rat prolactin in different organic systems
title_full_unstemmed Differential activity of 16K rat prolactin in different organic systems
title_short Differential activity of 16K rat prolactin in different organic systems
title_sort differential activity of 16k rat prolactin in different organic systems
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440500/
https://www.ncbi.nlm.nih.gov/pubmed/30949401
http://dx.doi.org/10.1080/19768354.2018.1554543
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