Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in Alcanivorax borkumensis SK2

Alcanivorax borkumensis is a ubiquitous marine bacterium that utilizes alkanes as a sole carbon source. We observed two phenotypes in the A. borkumensis SK2 type strain: rough (R) and smooth (S) types. The S type exhibited lower motility and higher polysaccharide production than the R type. Full gen...

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Autores principales: Prasad, Manoj, Obana, Nozomu, Sakai, Kaori, Nagakubo, Toshiki, Miyazaki, Shun, Toyofuku, Masanori, Fattaccioli, Jacques, Nomura, Nobuhiko, Utada, Andrew S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2019
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440734/
https://www.ncbi.nlm.nih.gov/pubmed/30773505
http://dx.doi.org/10.1264/jsme2.ME18151
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author Prasad, Manoj
Obana, Nozomu
Sakai, Kaori
Nagakubo, Toshiki
Miyazaki, Shun
Toyofuku, Masanori
Fattaccioli, Jacques
Nomura, Nobuhiko
Utada, Andrew S.
author_facet Prasad, Manoj
Obana, Nozomu
Sakai, Kaori
Nagakubo, Toshiki
Miyazaki, Shun
Toyofuku, Masanori
Fattaccioli, Jacques
Nomura, Nobuhiko
Utada, Andrew S.
author_sort Prasad, Manoj
collection PubMed
description Alcanivorax borkumensis is a ubiquitous marine bacterium that utilizes alkanes as a sole carbon source. We observed two phenotypes in the A. borkumensis SK2 type strain: rough (R) and smooth (S) types. The S type exhibited lower motility and higher polysaccharide production than the R type. Full genome sequencing revealed a mutation in the S type involved in cyclic-di-GMP production. The present results suggest that higher c-di-GMP levels in the S type control the biofilm forming behavior of this bacterium in a manner commensurate with other Gram-negative bacteria.
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spelling pubmed-64407342019-04-10 Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in Alcanivorax borkumensis SK2 Prasad, Manoj Obana, Nozomu Sakai, Kaori Nagakubo, Toshiki Miyazaki, Shun Toyofuku, Masanori Fattaccioli, Jacques Nomura, Nobuhiko Utada, Andrew S. Microbes Environ Short Communication Alcanivorax borkumensis is a ubiquitous marine bacterium that utilizes alkanes as a sole carbon source. We observed two phenotypes in the A. borkumensis SK2 type strain: rough (R) and smooth (S) types. The S type exhibited lower motility and higher polysaccharide production than the R type. Full genome sequencing revealed a mutation in the S type involved in cyclic-di-GMP production. The present results suggest that higher c-di-GMP levels in the S type control the biofilm forming behavior of this bacterium in a manner commensurate with other Gram-negative bacteria. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2019-03 2019-02-16 /pmc/articles/PMC6440734/ /pubmed/30773505 http://dx.doi.org/10.1264/jsme2.ME18151 Text en Copyright © 2019 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Prasad, Manoj
Obana, Nozomu
Sakai, Kaori
Nagakubo, Toshiki
Miyazaki, Shun
Toyofuku, Masanori
Fattaccioli, Jacques
Nomura, Nobuhiko
Utada, Andrew S.
Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in Alcanivorax borkumensis SK2
title Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in Alcanivorax borkumensis SK2
title_full Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in Alcanivorax borkumensis SK2
title_fullStr Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in Alcanivorax borkumensis SK2
title_full_unstemmed Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in Alcanivorax borkumensis SK2
title_short Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in Alcanivorax borkumensis SK2
title_sort point mutations lead to increased levels of c-di-gmp and phenotypic changes to the colony biofilm morphology in alcanivorax borkumensis sk2
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440734/
https://www.ncbi.nlm.nih.gov/pubmed/30773505
http://dx.doi.org/10.1264/jsme2.ME18151
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