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Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination

Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial r...

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Detalles Bibliográficos
Autores principales: Kuai, Yan, Chen, Junxue, Tang, Xi, Xiang, Yifeng, Lu, Fengya, Kuang, Cuifang, Xu, Liang, Shen, Weidong, Cheng, Junjie, Gui, Huaqiao, Zou, Gang, Wang, Pei, Ming, Hai, Liu, Jianguo, Liu, Xu, Lakowicz, Joseph R., Zhang, Douguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440756/
https://www.ncbi.nlm.nih.gov/pubmed/30944860
http://dx.doi.org/10.1126/sciadv.aav5335
Descripción
Sumario:Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers. The second is that SPRM requires use of metal films as sample substrates; this introduces working wavelength limitations. In addition, cell culture growth on metal films is not as universally available as growth on dielectric substrates. Here we show that use of azimuthal rotation illumination allows SPRM spatial resolution to be enhanced by up to an order of magnitude. The metal film can also be replaced by a dielectric multilayer and then a different label-free surface-sensitive photonic microscopy is developed, which has more choices in terms of the working wavelength, polarization, and imaging section, and will bring opportunities for applications in biology.