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Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination
Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial r...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440756/ https://www.ncbi.nlm.nih.gov/pubmed/30944860 http://dx.doi.org/10.1126/sciadv.aav5335 |
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author | Kuai, Yan Chen, Junxue Tang, Xi Xiang, Yifeng Lu, Fengya Kuang, Cuifang Xu, Liang Shen, Weidong Cheng, Junjie Gui, Huaqiao Zou, Gang Wang, Pei Ming, Hai Liu, Jianguo Liu, Xu Lakowicz, Joseph R. Zhang, Douguo |
author_facet | Kuai, Yan Chen, Junxue Tang, Xi Xiang, Yifeng Lu, Fengya Kuang, Cuifang Xu, Liang Shen, Weidong Cheng, Junjie Gui, Huaqiao Zou, Gang Wang, Pei Ming, Hai Liu, Jianguo Liu, Xu Lakowicz, Joseph R. Zhang, Douguo |
author_sort | Kuai, Yan |
collection | PubMed |
description | Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers. The second is that SPRM requires use of metal films as sample substrates; this introduces working wavelength limitations. In addition, cell culture growth on metal films is not as universally available as growth on dielectric substrates. Here we show that use of azimuthal rotation illumination allows SPRM spatial resolution to be enhanced by up to an order of magnitude. The metal film can also be replaced by a dielectric multilayer and then a different label-free surface-sensitive photonic microscopy is developed, which has more choices in terms of the working wavelength, polarization, and imaging section, and will bring opportunities for applications in biology. |
format | Online Article Text |
id | pubmed-6440756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64407562019-04-03 Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination Kuai, Yan Chen, Junxue Tang, Xi Xiang, Yifeng Lu, Fengya Kuang, Cuifang Xu, Liang Shen, Weidong Cheng, Junjie Gui, Huaqiao Zou, Gang Wang, Pei Ming, Hai Liu, Jianguo Liu, Xu Lakowicz, Joseph R. Zhang, Douguo Sci Adv Research Articles Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers. The second is that SPRM requires use of metal films as sample substrates; this introduces working wavelength limitations. In addition, cell culture growth on metal films is not as universally available as growth on dielectric substrates. Here we show that use of azimuthal rotation illumination allows SPRM spatial resolution to be enhanced by up to an order of magnitude. The metal film can also be replaced by a dielectric multilayer and then a different label-free surface-sensitive photonic microscopy is developed, which has more choices in terms of the working wavelength, polarization, and imaging section, and will bring opportunities for applications in biology. American Association for the Advancement of Science 2019-03-29 /pmc/articles/PMC6440756/ /pubmed/30944860 http://dx.doi.org/10.1126/sciadv.aav5335 Text en Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Kuai, Yan Chen, Junxue Tang, Xi Xiang, Yifeng Lu, Fengya Kuang, Cuifang Xu, Liang Shen, Weidong Cheng, Junjie Gui, Huaqiao Zou, Gang Wang, Pei Ming, Hai Liu, Jianguo Liu, Xu Lakowicz, Joseph R. Zhang, Douguo Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination |
title | Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination |
title_full | Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination |
title_fullStr | Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination |
title_full_unstemmed | Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination |
title_short | Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination |
title_sort | label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440756/ https://www.ncbi.nlm.nih.gov/pubmed/30944860 http://dx.doi.org/10.1126/sciadv.aav5335 |
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