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pH-induced morphological changes of proteinaceous viral shells

Changes in environmental pH can induce morphological changes in empty proteinaceous shells of bacteriophages in vitro that are very similar to changes occurring in viral capsids in vivo after encapsidation of DNA. These changes in capsid shape and size cannot be explained with a simple elastic model...

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Autores principales: Roshal, D., Konevtsova, O., Lošdorfer Božič, A., Podgornik, R., Rochal, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440952/
https://www.ncbi.nlm.nih.gov/pubmed/30926857
http://dx.doi.org/10.1038/s41598-019-41799-6
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author Roshal, D.
Konevtsova, O.
Lošdorfer Božič, A.
Podgornik, R.
Rochal, S.
author_facet Roshal, D.
Konevtsova, O.
Lošdorfer Božič, A.
Podgornik, R.
Rochal, S.
author_sort Roshal, D.
collection PubMed
description Changes in environmental pH can induce morphological changes in empty proteinaceous shells of bacteriophages in vitro that are very similar to changes occurring in viral capsids in vivo after encapsidation of DNA. These changes in capsid shape and size cannot be explained with a simple elastic model alone. We propose a new theoretical framework that combines the elasticity of thin icosahedral shells with the pH dependence of capsid charge distribution. Minimization of the sum of elastic and electrostatic free energies leads to equilibrium shapes of viral shells that depend on a single elastic parameter and the detailed configuration of the imbedded protein charges. Based on the in vitro shell reconstructions of bacteriophage HK97 we elucidate the details of how the reversible transition between Prohead II and Expansion Intermediate II states of the HK97 procapsid is induced by pH changes, as well as some other features of the bacteriophage maturation.
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spelling pubmed-64409522019-04-04 pH-induced morphological changes of proteinaceous viral shells Roshal, D. Konevtsova, O. Lošdorfer Božič, A. Podgornik, R. Rochal, S. Sci Rep Article Changes in environmental pH can induce morphological changes in empty proteinaceous shells of bacteriophages in vitro that are very similar to changes occurring in viral capsids in vivo after encapsidation of DNA. These changes in capsid shape and size cannot be explained with a simple elastic model alone. We propose a new theoretical framework that combines the elasticity of thin icosahedral shells with the pH dependence of capsid charge distribution. Minimization of the sum of elastic and electrostatic free energies leads to equilibrium shapes of viral shells that depend on a single elastic parameter and the detailed configuration of the imbedded protein charges. Based on the in vitro shell reconstructions of bacteriophage HK97 we elucidate the details of how the reversible transition between Prohead II and Expansion Intermediate II states of the HK97 procapsid is induced by pH changes, as well as some other features of the bacteriophage maturation. Nature Publishing Group UK 2019-03-29 /pmc/articles/PMC6440952/ /pubmed/30926857 http://dx.doi.org/10.1038/s41598-019-41799-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Roshal, D.
Konevtsova, O.
Lošdorfer Božič, A.
Podgornik, R.
Rochal, S.
pH-induced morphological changes of proteinaceous viral shells
title pH-induced morphological changes of proteinaceous viral shells
title_full pH-induced morphological changes of proteinaceous viral shells
title_fullStr pH-induced morphological changes of proteinaceous viral shells
title_full_unstemmed pH-induced morphological changes of proteinaceous viral shells
title_short pH-induced morphological changes of proteinaceous viral shells
title_sort ph-induced morphological changes of proteinaceous viral shells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440952/
https://www.ncbi.nlm.nih.gov/pubmed/30926857
http://dx.doi.org/10.1038/s41598-019-41799-6
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