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A coupled microscopy approach to assess the nano-landscape of weathering
Mineral weathering is a balanced interplay among physical, chemical, and biological processes. Fundamental knowledge gaps exist in characterizing the biogeochemical mechanisms that transform microbe-mineral interfaces at submicron scales, particularly in complex field systems. Our objective was to d...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441011/ https://www.ncbi.nlm.nih.gov/pubmed/30926847 http://dx.doi.org/10.1038/s41598-019-41357-0 |
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author | Lybrand, Rebecca A. Austin, Jason C. Fedenko, Jennifer Gallery, Rachel E. Rooney, Erin Schroeder, Paul A. Zaharescu, Dragos G. Qafoku, Odeta |
author_facet | Lybrand, Rebecca A. Austin, Jason C. Fedenko, Jennifer Gallery, Rachel E. Rooney, Erin Schroeder, Paul A. Zaharescu, Dragos G. Qafoku, Odeta |
author_sort | Lybrand, Rebecca A. |
collection | PubMed |
description | Mineral weathering is a balanced interplay among physical, chemical, and biological processes. Fundamental knowledge gaps exist in characterizing the biogeochemical mechanisms that transform microbe-mineral interfaces at submicron scales, particularly in complex field systems. Our objective was to develop methods targeting the nanoscale by using high-resolution microscopy to assess biological and geochemical drivers of weathering in natural settings. Basalt, granite, and quartz (53–250 µm) were deployed in surface soils (10 cm) of three ecosystems (semiarid, subhumid, humid) for one year. We successfully developed a reference grid method to analyze individual grains using: (1) helium ion microscopy to capture micron to sub-nanometer imagery of mineral-organic interactions; and (2) scanning electron microscopy to quantify elemental distribution on the same surfaces via element mapping and point analyses. We detected locations of biomechanical weathering, secondary mineral precipitation, biofilm formation, and grain coatings across the three contrasting climates. To our knowledge, this is the first time these coupled microscopy techniques were applied in the earth and ecosystem sciences to assess microbe-mineral interfaces and in situ biological contributors to incipient weathering. |
format | Online Article Text |
id | pubmed-6441011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-64410112019-04-04 A coupled microscopy approach to assess the nano-landscape of weathering Lybrand, Rebecca A. Austin, Jason C. Fedenko, Jennifer Gallery, Rachel E. Rooney, Erin Schroeder, Paul A. Zaharescu, Dragos G. Qafoku, Odeta Sci Rep Article Mineral weathering is a balanced interplay among physical, chemical, and biological processes. Fundamental knowledge gaps exist in characterizing the biogeochemical mechanisms that transform microbe-mineral interfaces at submicron scales, particularly in complex field systems. Our objective was to develop methods targeting the nanoscale by using high-resolution microscopy to assess biological and geochemical drivers of weathering in natural settings. Basalt, granite, and quartz (53–250 µm) were deployed in surface soils (10 cm) of three ecosystems (semiarid, subhumid, humid) for one year. We successfully developed a reference grid method to analyze individual grains using: (1) helium ion microscopy to capture micron to sub-nanometer imagery of mineral-organic interactions; and (2) scanning electron microscopy to quantify elemental distribution on the same surfaces via element mapping and point analyses. We detected locations of biomechanical weathering, secondary mineral precipitation, biofilm formation, and grain coatings across the three contrasting climates. To our knowledge, this is the first time these coupled microscopy techniques were applied in the earth and ecosystem sciences to assess microbe-mineral interfaces and in situ biological contributors to incipient weathering. Nature Publishing Group UK 2019-03-29 /pmc/articles/PMC6441011/ /pubmed/30926847 http://dx.doi.org/10.1038/s41598-019-41357-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lybrand, Rebecca A. Austin, Jason C. Fedenko, Jennifer Gallery, Rachel E. Rooney, Erin Schroeder, Paul A. Zaharescu, Dragos G. Qafoku, Odeta A coupled microscopy approach to assess the nano-landscape of weathering |
title | A coupled microscopy approach to assess the nano-landscape of weathering |
title_full | A coupled microscopy approach to assess the nano-landscape of weathering |
title_fullStr | A coupled microscopy approach to assess the nano-landscape of weathering |
title_full_unstemmed | A coupled microscopy approach to assess the nano-landscape of weathering |
title_short | A coupled microscopy approach to assess the nano-landscape of weathering |
title_sort | coupled microscopy approach to assess the nano-landscape of weathering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441011/ https://www.ncbi.nlm.nih.gov/pubmed/30926847 http://dx.doi.org/10.1038/s41598-019-41357-0 |
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