The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression

Suppressor of Hairy-wing [Su(Hw)] is a DNA-binding architectural protein that participates in the organization of insulators and repression of promoters in Drosophila. This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in the...

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Autores principales: Melnikova, Larisa, Elizar’ev, Pavel, Erokhin, Maksim, Molodina, Varvara, Chetverina, Darya, Kostyuchenko, Margarita, Georgiev, Pavel, Golovnin, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441048/
https://www.ncbi.nlm.nih.gov/pubmed/30926937
http://dx.doi.org/10.1038/s41598-019-41761-6
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author Melnikova, Larisa
Elizar’ev, Pavel
Erokhin, Maksim
Molodina, Varvara
Chetverina, Darya
Kostyuchenko, Margarita
Georgiev, Pavel
Golovnin, Anton
author_facet Melnikova, Larisa
Elizar’ev, Pavel
Erokhin, Maksim
Molodina, Varvara
Chetverina, Darya
Kostyuchenko, Margarita
Georgiev, Pavel
Golovnin, Anton
author_sort Melnikova, Larisa
collection PubMed
description Suppressor of Hairy-wing [Su(Hw)] is a DNA-binding architectural protein that participates in the organization of insulators and repression of promoters in Drosophila. This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in the specific recognition of the binding site. One of the well-described in vivo function of Su(Hw) is the repression of transcription of neuronal genes in oocytes. Here, we have found that the same Su(Hw) C-terminal region (aa 720–892) is required for insulation as well as for promoter repression. The best characterized partners of Su(Hw), CP190 and Mod(mdg4)-67.2, are not involved in the repression of neuronal genes. Taken together, these results suggest that an unknown protein or protein complex binds to the C-terminal region of Su(Hw) and is responsible for the direct repression activity of Su(Hw).
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spelling pubmed-64410482019-04-04 The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression Melnikova, Larisa Elizar’ev, Pavel Erokhin, Maksim Molodina, Varvara Chetverina, Darya Kostyuchenko, Margarita Georgiev, Pavel Golovnin, Anton Sci Rep Article Suppressor of Hairy-wing [Su(Hw)] is a DNA-binding architectural protein that participates in the organization of insulators and repression of promoters in Drosophila. This protein contains acidic regions at both ends and a central cluster of 12 zinc finger domains, some of which are involved in the specific recognition of the binding site. One of the well-described in vivo function of Su(Hw) is the repression of transcription of neuronal genes in oocytes. Here, we have found that the same Su(Hw) C-terminal region (aa 720–892) is required for insulation as well as for promoter repression. The best characterized partners of Su(Hw), CP190 and Mod(mdg4)-67.2, are not involved in the repression of neuronal genes. Taken together, these results suggest that an unknown protein or protein complex binds to the C-terminal region of Su(Hw) and is responsible for the direct repression activity of Su(Hw). Nature Publishing Group UK 2019-03-29 /pmc/articles/PMC6441048/ /pubmed/30926937 http://dx.doi.org/10.1038/s41598-019-41761-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Melnikova, Larisa
Elizar’ev, Pavel
Erokhin, Maksim
Molodina, Varvara
Chetverina, Darya
Kostyuchenko, Margarita
Georgiev, Pavel
Golovnin, Anton
The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression
title The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression
title_full The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression
title_fullStr The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression
title_full_unstemmed The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression
title_short The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression
title_sort same domain of su(hw) is required for enhancer blocking and direct promoter repression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441048/
https://www.ncbi.nlm.nih.gov/pubmed/30926937
http://dx.doi.org/10.1038/s41598-019-41761-6
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