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Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome

To investigate the ratio of mitochondrial DNA to genomic DNA (mt/gDNA) in embryo culture medium as a possible predictor for embryonic development and pregnancy outcome, we collected a total of 93 embryo biopsy specimens from 52 women at the corresponding Day 3 (D3) and Day 5 (D5) embryo culture medi...

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Autores principales: Zhang, Xinyue, Sun, Yue, Dong, Xin, Zhou, Jianming, Sun, Fubo, Han, Tingting, Lei, Ping, Mao, Rurong, Guo, Xuzhou, Wang, Qi, Li, Penghao, Qu, Ting, Huang, Jihua, Li, Lingxiao, Huang, Tianhua, Zhong, Ying, Gu, Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441050/
https://www.ncbi.nlm.nih.gov/pubmed/30926852
http://dx.doi.org/10.1038/s41598-019-41801-1
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author Zhang, Xinyue
Sun, Yue
Dong, Xin
Zhou, Jianming
Sun, Fubo
Han, Tingting
Lei, Ping
Mao, Rurong
Guo, Xuzhou
Wang, Qi
Li, Penghao
Qu, Ting
Huang, Jihua
Li, Lingxiao
Huang, Tianhua
Zhong, Ying
Gu, Jiang
author_facet Zhang, Xinyue
Sun, Yue
Dong, Xin
Zhou, Jianming
Sun, Fubo
Han, Tingting
Lei, Ping
Mao, Rurong
Guo, Xuzhou
Wang, Qi
Li, Penghao
Qu, Ting
Huang, Jihua
Li, Lingxiao
Huang, Tianhua
Zhong, Ying
Gu, Jiang
author_sort Zhang, Xinyue
collection PubMed
description To investigate the ratio of mitochondrial DNA to genomic DNA (mt/gDNA) in embryo culture medium as a possible predictor for embryonic development and pregnancy outcome, we collected a total of 93 embryo biopsy specimens from 52 women at the corresponding Day 3 (D3) and Day 5 (D5) embryo culture medium of in vitro fertilization. With the multiple annealing and looping-based amplification cycles method of next-generation sequencing for whole genome amplification, we examined the karyotype of the biopsy samples and the mt/gDNA ratio in the culture medium. Results showed that the ratio of mt/gDNA had an upward trend with decreasing trophectoderm levels with no significant difference. At the same time, from D3 to D5, the mt/gDNA ratio in the medium of embryos that failed to become blastocysts showed an upward trend, and the mt/gDNA ratio of medium from embryos that reached blastulation with successful pregnancy showed a decreasing trend, but the differences were not statistically significant. We conclude that there is a certain correlation between mt/gDNA ratio and early embryonic development, but it does not reach a level that can be used as a clinical predictor.
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spelling pubmed-64410502019-04-04 Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome Zhang, Xinyue Sun, Yue Dong, Xin Zhou, Jianming Sun, Fubo Han, Tingting Lei, Ping Mao, Rurong Guo, Xuzhou Wang, Qi Li, Penghao Qu, Ting Huang, Jihua Li, Lingxiao Huang, Tianhua Zhong, Ying Gu, Jiang Sci Rep Article To investigate the ratio of mitochondrial DNA to genomic DNA (mt/gDNA) in embryo culture medium as a possible predictor for embryonic development and pregnancy outcome, we collected a total of 93 embryo biopsy specimens from 52 women at the corresponding Day 3 (D3) and Day 5 (D5) embryo culture medium of in vitro fertilization. With the multiple annealing and looping-based amplification cycles method of next-generation sequencing for whole genome amplification, we examined the karyotype of the biopsy samples and the mt/gDNA ratio in the culture medium. Results showed that the ratio of mt/gDNA had an upward trend with decreasing trophectoderm levels with no significant difference. At the same time, from D3 to D5, the mt/gDNA ratio in the medium of embryos that failed to become blastocysts showed an upward trend, and the mt/gDNA ratio of medium from embryos that reached blastulation with successful pregnancy showed a decreasing trend, but the differences were not statistically significant. We conclude that there is a certain correlation between mt/gDNA ratio and early embryonic development, but it does not reach a level that can be used as a clinical predictor. Nature Publishing Group UK 2019-03-29 /pmc/articles/PMC6441050/ /pubmed/30926852 http://dx.doi.org/10.1038/s41598-019-41801-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Xinyue
Sun, Yue
Dong, Xin
Zhou, Jianming
Sun, Fubo
Han, Tingting
Lei, Ping
Mao, Rurong
Guo, Xuzhou
Wang, Qi
Li, Penghao
Qu, Ting
Huang, Jihua
Li, Lingxiao
Huang, Tianhua
Zhong, Ying
Gu, Jiang
Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome
title Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome
title_full Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome
title_fullStr Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome
title_full_unstemmed Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome
title_short Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome
title_sort mitochondrial dna and genomic dna ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441050/
https://www.ncbi.nlm.nih.gov/pubmed/30926852
http://dx.doi.org/10.1038/s41598-019-41801-1
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