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Conditioned stimulus presentations alter anxiety level in fear-conditioned mice

It is generally believed that fear is rapidly triggered by a distinct cue while anxiety onset is less precise and not associated with a distinct cue. Although it has been claimed that both processes can be measured with certain independence of each other, it is unclear how exactly they differ. In th...

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Detalles Bibliográficos
Autores principales: Zhang, Yujie, Ouyang, Kunfu, Lipina, Tatiana V, Wang, Hong, Zhou, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441152/
https://www.ncbi.nlm.nih.gov/pubmed/30925893
http://dx.doi.org/10.1186/s13041-019-0445-4
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author Zhang, Yujie
Ouyang, Kunfu
Lipina, Tatiana V
Wang, Hong
Zhou, Qiang
author_facet Zhang, Yujie
Ouyang, Kunfu
Lipina, Tatiana V
Wang, Hong
Zhou, Qiang
author_sort Zhang, Yujie
collection PubMed
description It is generally believed that fear is rapidly triggered by a distinct cue while anxiety onset is less precise and not associated with a distinct cue. Although it has been claimed that both processes can be measured with certain independence of each other, it is unclear how exactly they differ. In this study, we measured anxiety in mice that received discriminative fear conditioning using behavioral, heart rate and calcium (Ca(2+)) responses in the ventral hippocampal CA1 (vCA1) neurons. We found that the occurrence of fear significantly interfered with anxiety measurements under various conditions. Diazepam reduced basal anxiety level but had no effect during the presentation of conditioned stimulus (CS). Injection of an inhibitory peptide of PKMzeta (ZIP) into the basolateral amygdala almost entirely abolished CS-triggered fear expression and reduced anxiety to basal level. Heart rate measures suggested a small reduction in anxiety during CS-. Calcium responses in the lateral hypothalamus-projecting vCA1 neurons showed a steady decay during CS suggesting a reduced anxiety. Thus, under our experimental conditions, CS presentations likely reduce anxiety level in the fear-conditioned mice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13041-019-0445-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-64411522019-04-11 Conditioned stimulus presentations alter anxiety level in fear-conditioned mice Zhang, Yujie Ouyang, Kunfu Lipina, Tatiana V Wang, Hong Zhou, Qiang Mol Brain Research It is generally believed that fear is rapidly triggered by a distinct cue while anxiety onset is less precise and not associated with a distinct cue. Although it has been claimed that both processes can be measured with certain independence of each other, it is unclear how exactly they differ. In this study, we measured anxiety in mice that received discriminative fear conditioning using behavioral, heart rate and calcium (Ca(2+)) responses in the ventral hippocampal CA1 (vCA1) neurons. We found that the occurrence of fear significantly interfered with anxiety measurements under various conditions. Diazepam reduced basal anxiety level but had no effect during the presentation of conditioned stimulus (CS). Injection of an inhibitory peptide of PKMzeta (ZIP) into the basolateral amygdala almost entirely abolished CS-triggered fear expression and reduced anxiety to basal level. Heart rate measures suggested a small reduction in anxiety during CS-. Calcium responses in the lateral hypothalamus-projecting vCA1 neurons showed a steady decay during CS suggesting a reduced anxiety. Thus, under our experimental conditions, CS presentations likely reduce anxiety level in the fear-conditioned mice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13041-019-0445-4) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-29 /pmc/articles/PMC6441152/ /pubmed/30925893 http://dx.doi.org/10.1186/s13041-019-0445-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Yujie
Ouyang, Kunfu
Lipina, Tatiana V
Wang, Hong
Zhou, Qiang
Conditioned stimulus presentations alter anxiety level in fear-conditioned mice
title Conditioned stimulus presentations alter anxiety level in fear-conditioned mice
title_full Conditioned stimulus presentations alter anxiety level in fear-conditioned mice
title_fullStr Conditioned stimulus presentations alter anxiety level in fear-conditioned mice
title_full_unstemmed Conditioned stimulus presentations alter anxiety level in fear-conditioned mice
title_short Conditioned stimulus presentations alter anxiety level in fear-conditioned mice
title_sort conditioned stimulus presentations alter anxiety level in fear-conditioned mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441152/
https://www.ncbi.nlm.nih.gov/pubmed/30925893
http://dx.doi.org/10.1186/s13041-019-0445-4
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