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The identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation
BACKGROUND: Placental efficiency (PE) describes the relationship between placental and fetal weights (fetal wt/placental wt). Within litters, PE can vary drastically, resulting in similarly sized pigs associated with differently sized placentas, up to a 25% weight difference. However, the mechanisms...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441153/ https://www.ncbi.nlm.nih.gov/pubmed/30925895 http://dx.doi.org/10.1186/s12864-019-5626-0 |
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author | Krombeen, Shanice K. Shankar, Vijay Noorai, Rooksana E. Saski, Christopher A. Sharp, Julia L. Wilson, Matthew E. Wilmoth, Tiffany A. |
author_facet | Krombeen, Shanice K. Shankar, Vijay Noorai, Rooksana E. Saski, Christopher A. Sharp, Julia L. Wilson, Matthew E. Wilmoth, Tiffany A. |
author_sort | Krombeen, Shanice K. |
collection | PubMed |
description | BACKGROUND: Placental efficiency (PE) describes the relationship between placental and fetal weights (fetal wt/placental wt). Within litters, PE can vary drastically, resulting in similarly sized pigs associated with differently sized placentas, up to a 25% weight difference. However, the mechanisms enabling the smaller placenta to grow a comparable littermate are unknown. To elucidate potential mechanisms, morphological measurements and gene expression profiles in placental and associated endometrial tissues of high PE and low PE feto-placental units were compared. Tissue samples were obtained from eight maternal line gilts during gestational day 95 ovario-hysterectomies. RNA was extracted from tissues of feto-placental units with the highest and lowest PE in each litter and sequenced. RESULTS: Morphological measurements, except placental weight, were not different (P > 0.05) between high and low PE. No DEG were identified in the endometrium and 214 DEG were identified in the placenta (FDR < 0.1), of which 48% were upregulated and 52% were downregulated. Gene ontology (GO) analysis revealed that a large percentage of DEG were involved in catalytic activity, binding, transporter activity, metabolism, biological regulation, and localization. Four GO terms were enriched in the upregulated genes and no terms were enriched in the downregulated genes (FDR < 0.05). Eight statistically significant correlations (P < 0.05) were identified between the morphological measurements and DEG. CONCLUSION: Morphological measures between high and low PE verified comparisons were of similarly sized pigs grown on different sized placentas, and indicated that any negative effects of a reduced placental size on fetal growth were not evident by day 95. The identification of DEG in the placenta, but absence of DEG in the endometrium confirmed that the placenta responds to the fetus. The GO analyses provided evidence that extremes of PE are differentially regulated, affecting components of placental transport capacity like nutrient transport and blood flow. However, alternative GO terms were identified, indicating the complexity of the relationship between placental and fetal weights. These findings support the use of PE as a marker of placental function and provide novel insight into the genetic control of PE, but further research is required to make PE production applicable. |
format | Online Article Text |
id | pubmed-6441153 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-64411532019-04-11 The identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation Krombeen, Shanice K. Shankar, Vijay Noorai, Rooksana E. Saski, Christopher A. Sharp, Julia L. Wilson, Matthew E. Wilmoth, Tiffany A. BMC Genomics Research Article BACKGROUND: Placental efficiency (PE) describes the relationship between placental and fetal weights (fetal wt/placental wt). Within litters, PE can vary drastically, resulting in similarly sized pigs associated with differently sized placentas, up to a 25% weight difference. However, the mechanisms enabling the smaller placenta to grow a comparable littermate are unknown. To elucidate potential mechanisms, morphological measurements and gene expression profiles in placental and associated endometrial tissues of high PE and low PE feto-placental units were compared. Tissue samples were obtained from eight maternal line gilts during gestational day 95 ovario-hysterectomies. RNA was extracted from tissues of feto-placental units with the highest and lowest PE in each litter and sequenced. RESULTS: Morphological measurements, except placental weight, were not different (P > 0.05) between high and low PE. No DEG were identified in the endometrium and 214 DEG were identified in the placenta (FDR < 0.1), of which 48% were upregulated and 52% were downregulated. Gene ontology (GO) analysis revealed that a large percentage of DEG were involved in catalytic activity, binding, transporter activity, metabolism, biological regulation, and localization. Four GO terms were enriched in the upregulated genes and no terms were enriched in the downregulated genes (FDR < 0.05). Eight statistically significant correlations (P < 0.05) were identified between the morphological measurements and DEG. CONCLUSION: Morphological measures between high and low PE verified comparisons were of similarly sized pigs grown on different sized placentas, and indicated that any negative effects of a reduced placental size on fetal growth were not evident by day 95. The identification of DEG in the placenta, but absence of DEG in the endometrium confirmed that the placenta responds to the fetus. The GO analyses provided evidence that extremes of PE are differentially regulated, affecting components of placental transport capacity like nutrient transport and blood flow. However, alternative GO terms were identified, indicating the complexity of the relationship between placental and fetal weights. These findings support the use of PE as a marker of placental function and provide novel insight into the genetic control of PE, but further research is required to make PE production applicable. BioMed Central 2019-03-29 /pmc/articles/PMC6441153/ /pubmed/30925895 http://dx.doi.org/10.1186/s12864-019-5626-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Krombeen, Shanice K. Shankar, Vijay Noorai, Rooksana E. Saski, Christopher A. Sharp, Julia L. Wilson, Matthew E. Wilmoth, Tiffany A. The identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation |
title | The identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation |
title_full | The identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation |
title_fullStr | The identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation |
title_full_unstemmed | The identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation |
title_short | The identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation |
title_sort | identification of differentially expressed genes between extremes of placental efficiency in maternal line gilts on day 95 of gestation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441153/ https://www.ncbi.nlm.nih.gov/pubmed/30925895 http://dx.doi.org/10.1186/s12864-019-5626-0 |
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