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The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma

BACKGROUND: The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma (LUAD) is unclear. METHODS: A total of 506 lung adenocarcinoma samples from The Cancer Genome Atlas (TCGA) dataset and 173 LUAD tumour tissues from Jiangxi Cancer Hospital were used to analyse...

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Autores principales: Guo, Chang-Ying, Zhu, Qian, Tou, Fang-Fang, Wen, Xiao-Ming, Kuang, Yu-Kang, Hu, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441172/
https://www.ncbi.nlm.nih.gov/pubmed/30925904
http://dx.doi.org/10.1186/s12885-019-5519-2
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author Guo, Chang-Ying
Zhu, Qian
Tou, Fang-Fang
Wen, Xiao-Ming
Kuang, Yu-Kang
Hu, Hao
author_facet Guo, Chang-Ying
Zhu, Qian
Tou, Fang-Fang
Wen, Xiao-Ming
Kuang, Yu-Kang
Hu, Hao
author_sort Guo, Chang-Ying
collection PubMed
description BACKGROUND: The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma (LUAD) is unclear. METHODS: A total of 506 lung adenocarcinoma samples from The Cancer Genome Atlas (TCGA) dataset and 173 LUAD tumour tissues from Jiangxi Cancer Hospital were used to analyse the correlation between PKM2 and PD-L1 expression. We further established a stable LUAD cell line with PKM2 knockdown and confirmed the association via Western blotting and flow cytometry analysis. Moreover, the prognostic values of PKM2 and PD-L1 were evaluated by the Kaplan-Meier method and Cox proportional hazards models. RESULTS: Based on the above two large cohorts, we found that PKM2 was significantly positively associated with PD-L1 expression (r = 0.132, P = 0.003 and r = 0.287, P < 0.001, respectively). Subsequently, we found that PKM2 knockdown substantially inhibited PD-L1 expression in the A549 LUAD cell line. Moreover, survival analysis showed that higher expression of PKM2 was correlated with significantly shorter overall survival (OS) and disease-free survival (DFS) in lung adenocarcinoma patients (P < 0.001 and P = 0.050, respectively). Subgroup analysis showed that lung adenocarcinoma patients who expressed high PKM2 and PD-L1 levels experienced the poorest OS and DFS. Additionally, multivariate analysis suggested that high PKM2 and PD-L1 expression was an independent prognostic indicator for worse OS and DFS (HR = 1.462, P < 0.001 and HR = 1.436, P = 0.004, respectively). CONCLUSIONS: Our results demonstrated that PKM2 regulated PD-L1 expression and was associated with poor outcomes in lung adenocarcinoma patients.
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spelling pubmed-64411722019-04-11 The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma Guo, Chang-Ying Zhu, Qian Tou, Fang-Fang Wen, Xiao-Ming Kuang, Yu-Kang Hu, Hao BMC Cancer Research Article BACKGROUND: The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma (LUAD) is unclear. METHODS: A total of 506 lung adenocarcinoma samples from The Cancer Genome Atlas (TCGA) dataset and 173 LUAD tumour tissues from Jiangxi Cancer Hospital were used to analyse the correlation between PKM2 and PD-L1 expression. We further established a stable LUAD cell line with PKM2 knockdown and confirmed the association via Western blotting and flow cytometry analysis. Moreover, the prognostic values of PKM2 and PD-L1 were evaluated by the Kaplan-Meier method and Cox proportional hazards models. RESULTS: Based on the above two large cohorts, we found that PKM2 was significantly positively associated with PD-L1 expression (r = 0.132, P = 0.003 and r = 0.287, P < 0.001, respectively). Subsequently, we found that PKM2 knockdown substantially inhibited PD-L1 expression in the A549 LUAD cell line. Moreover, survival analysis showed that higher expression of PKM2 was correlated with significantly shorter overall survival (OS) and disease-free survival (DFS) in lung adenocarcinoma patients (P < 0.001 and P = 0.050, respectively). Subgroup analysis showed that lung adenocarcinoma patients who expressed high PKM2 and PD-L1 levels experienced the poorest OS and DFS. Additionally, multivariate analysis suggested that high PKM2 and PD-L1 expression was an independent prognostic indicator for worse OS and DFS (HR = 1.462, P < 0.001 and HR = 1.436, P = 0.004, respectively). CONCLUSIONS: Our results demonstrated that PKM2 regulated PD-L1 expression and was associated with poor outcomes in lung adenocarcinoma patients. BioMed Central 2019-03-29 /pmc/articles/PMC6441172/ /pubmed/30925904 http://dx.doi.org/10.1186/s12885-019-5519-2 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Guo, Chang-Ying
Zhu, Qian
Tou, Fang-Fang
Wen, Xiao-Ming
Kuang, Yu-Kang
Hu, Hao
The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma
title The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma
title_full The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma
title_fullStr The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma
title_full_unstemmed The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma
title_short The prognostic value of PKM2 and its correlation with tumour cell PD-L1 in lung adenocarcinoma
title_sort prognostic value of pkm2 and its correlation with tumour cell pd-l1 in lung adenocarcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441172/
https://www.ncbi.nlm.nih.gov/pubmed/30925904
http://dx.doi.org/10.1186/s12885-019-5519-2
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