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A gene variation of Interferon Gamma Receptor-I promoter (rs1327474A>G) and chronic hepatitis C virus infection

AIM: In present survey, we attempted to inquire the plausible linkage of rs1327474 A/G and HCV chronic infection or the clearance of the virus. BACKGROUND: IFN-γ signaling pathway is an important trigger for activating antiviral immune responses and production of wide variety of molecules with anti-...

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Autores principales: Karkhane, Maryam, Mohebbi, Seyed Reza, Sharifian, Afsaneh, Ghaemi, Amir, Asadzadeh Aghdaei, Hamid, Zali, Mohammad Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441488/
https://www.ncbi.nlm.nih.gov/pubmed/30949319
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author Karkhane, Maryam
Mohebbi, Seyed Reza
Sharifian, Afsaneh
Ghaemi, Amir
Asadzadeh Aghdaei, Hamid
Zali, Mohammad Reza
author_facet Karkhane, Maryam
Mohebbi, Seyed Reza
Sharifian, Afsaneh
Ghaemi, Amir
Asadzadeh Aghdaei, Hamid
Zali, Mohammad Reza
author_sort Karkhane, Maryam
collection PubMed
description AIM: In present survey, we attempted to inquire the plausible linkage of rs1327474 A/G and HCV chronic infection or the clearance of the virus. BACKGROUND: IFN-γ signaling pathway is an important trigger for activating antiviral immune responses and production of wide variety of molecules with anti-microbial profiles including type 1 cytokines. Any defect or variation in IFNG signaling pathway may result in susceptibility or progression to diverse diseases such as inflammatory and virus associated disorders. Rs1327474 A/G also known as -611 A/G is an important variation which is located in promoter region of Interferon Gamma Receptor-I (IFNGR1) and may have potent risk for HCV susceptibility. METHODS: For this purpose, 154 HCV patients and 200 controls were enrolled in the study, and genomic DNA was isolated from PBMCS and IFNGR1 -611 polymorphism was genotyped by polymerase chain reaction- fragments length polymorphism (PCR-RFLP) method. RESULTS: While, AA, AG and GG genotypes frequency included 37.8%, 53.7%, 8.5% in healthy controls, 41.6%, 46.1%, 12.3% were found in chronic HCV patients. Interestingly, allelic percentage was similar in both separated groups within 64.7%, 35.3% and 65.3%, 34.7% were obtained for T and G allele in control and case group respectively. CONCLUSION: In spite of our exception for the possible role of this variation in an important promoter region of IFGR1 gene, rs1327474 A/G was not associated with HCV chronic infection among an Iranian studied group. Comprehensively, -611A/G cannot be considered as a risk biomarker for susceptibility to chronic HCV disease.
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spelling pubmed-64414882019-04-04 A gene variation of Interferon Gamma Receptor-I promoter (rs1327474A>G) and chronic hepatitis C virus infection Karkhane, Maryam Mohebbi, Seyed Reza Sharifian, Afsaneh Ghaemi, Amir Asadzadeh Aghdaei, Hamid Zali, Mohammad Reza Gastroenterol Hepatol Bed Bench Original Article AIM: In present survey, we attempted to inquire the plausible linkage of rs1327474 A/G and HCV chronic infection or the clearance of the virus. BACKGROUND: IFN-γ signaling pathway is an important trigger for activating antiviral immune responses and production of wide variety of molecules with anti-microbial profiles including type 1 cytokines. Any defect or variation in IFNG signaling pathway may result in susceptibility or progression to diverse diseases such as inflammatory and virus associated disorders. Rs1327474 A/G also known as -611 A/G is an important variation which is located in promoter region of Interferon Gamma Receptor-I (IFNGR1) and may have potent risk for HCV susceptibility. METHODS: For this purpose, 154 HCV patients and 200 controls were enrolled in the study, and genomic DNA was isolated from PBMCS and IFNGR1 -611 polymorphism was genotyped by polymerase chain reaction- fragments length polymorphism (PCR-RFLP) method. RESULTS: While, AA, AG and GG genotypes frequency included 37.8%, 53.7%, 8.5% in healthy controls, 41.6%, 46.1%, 12.3% were found in chronic HCV patients. Interestingly, allelic percentage was similar in both separated groups within 64.7%, 35.3% and 65.3%, 34.7% were obtained for T and G allele in control and case group respectively. CONCLUSION: In spite of our exception for the possible role of this variation in an important promoter region of IFGR1 gene, rs1327474 A/G was not associated with HCV chronic infection among an Iranian studied group. Comprehensively, -611A/G cannot be considered as a risk biomarker for susceptibility to chronic HCV disease. Shaheed Beheshti University of Medical Sciences 2019 /pmc/articles/PMC6441488/ /pubmed/30949319 Text en ©2019 RIGLD, Research Institute for Gastroenterology and Liver Diseases This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Karkhane, Maryam
Mohebbi, Seyed Reza
Sharifian, Afsaneh
Ghaemi, Amir
Asadzadeh Aghdaei, Hamid
Zali, Mohammad Reza
A gene variation of Interferon Gamma Receptor-I promoter (rs1327474A>G) and chronic hepatitis C virus infection
title A gene variation of Interferon Gamma Receptor-I promoter (rs1327474A>G) and chronic hepatitis C virus infection
title_full A gene variation of Interferon Gamma Receptor-I promoter (rs1327474A>G) and chronic hepatitis C virus infection
title_fullStr A gene variation of Interferon Gamma Receptor-I promoter (rs1327474A>G) and chronic hepatitis C virus infection
title_full_unstemmed A gene variation of Interferon Gamma Receptor-I promoter (rs1327474A>G) and chronic hepatitis C virus infection
title_short A gene variation of Interferon Gamma Receptor-I promoter (rs1327474A>G) and chronic hepatitis C virus infection
title_sort gene variation of interferon gamma receptor-i promoter (rs1327474a>g) and chronic hepatitis c virus infection
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441488/
https://www.ncbi.nlm.nih.gov/pubmed/30949319
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