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Epidermal development requires ninein for spindle orientation and cortical microtubule organization

In mammalian skin, ninein localizes to the centrosomes of progenitor cells and relocates to the cell cortex upon differentiation of keratinocytes, where cortical arrays of microtubules are formed. To examine the function of ninein in skin development, we use epidermis-specific and constitutive ninei...

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Autores principales: Lecland, Nicolas, Hsu, Chiung-Yueh, Chemin, Cécile, Merdes, Andreas, Bierkamp, Christiane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441496/
https://www.ncbi.nlm.nih.gov/pubmed/30923192
http://dx.doi.org/10.26508/lsa.201900373
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author Lecland, Nicolas
Hsu, Chiung-Yueh
Chemin, Cécile
Merdes, Andreas
Bierkamp, Christiane
author_facet Lecland, Nicolas
Hsu, Chiung-Yueh
Chemin, Cécile
Merdes, Andreas
Bierkamp, Christiane
author_sort Lecland, Nicolas
collection PubMed
description In mammalian skin, ninein localizes to the centrosomes of progenitor cells and relocates to the cell cortex upon differentiation of keratinocytes, where cortical arrays of microtubules are formed. To examine the function of ninein in skin development, we use epidermis-specific and constitutive ninein-knockout mice to demonstrate that ninein is necessary for maintaining regular protein levels of the differentiation markers filaggrin and involucrin, for the formation of desmosomes, for the secretion of lamellar bodies, and for the formation of the epidermal barrier. Ninein-deficient mice are viable but develop a thinner skin with partly impaired epidermal barrier. We propose two underlying mechanisms: first, ninein contributes to spindle orientation during the division of progenitor cells, whereas its absence leads to misoriented cell divisions, altering the pool of progenitor cells. Second, ninein is required for the cortical organization of microtubules in differentiating keratinocytes, and for the cortical re-localization of microtubule-organizing proteins, and may thus affect any mechanisms that depend on localized microtubule-dependent transport.
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spelling pubmed-64414962019-04-03 Epidermal development requires ninein for spindle orientation and cortical microtubule organization Lecland, Nicolas Hsu, Chiung-Yueh Chemin, Cécile Merdes, Andreas Bierkamp, Christiane Life Sci Alliance Research Articles In mammalian skin, ninein localizes to the centrosomes of progenitor cells and relocates to the cell cortex upon differentiation of keratinocytes, where cortical arrays of microtubules are formed. To examine the function of ninein in skin development, we use epidermis-specific and constitutive ninein-knockout mice to demonstrate that ninein is necessary for maintaining regular protein levels of the differentiation markers filaggrin and involucrin, for the formation of desmosomes, for the secretion of lamellar bodies, and for the formation of the epidermal barrier. Ninein-deficient mice are viable but develop a thinner skin with partly impaired epidermal barrier. We propose two underlying mechanisms: first, ninein contributes to spindle orientation during the division of progenitor cells, whereas its absence leads to misoriented cell divisions, altering the pool of progenitor cells. Second, ninein is required for the cortical organization of microtubules in differentiating keratinocytes, and for the cortical re-localization of microtubule-organizing proteins, and may thus affect any mechanisms that depend on localized microtubule-dependent transport. Life Science Alliance LLC 2019-03-28 /pmc/articles/PMC6441496/ /pubmed/30923192 http://dx.doi.org/10.26508/lsa.201900373 Text en © 2019 Lecland et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Lecland, Nicolas
Hsu, Chiung-Yueh
Chemin, Cécile
Merdes, Andreas
Bierkamp, Christiane
Epidermal development requires ninein for spindle orientation and cortical microtubule organization
title Epidermal development requires ninein for spindle orientation and cortical microtubule organization
title_full Epidermal development requires ninein for spindle orientation and cortical microtubule organization
title_fullStr Epidermal development requires ninein for spindle orientation and cortical microtubule organization
title_full_unstemmed Epidermal development requires ninein for spindle orientation and cortical microtubule organization
title_short Epidermal development requires ninein for spindle orientation and cortical microtubule organization
title_sort epidermal development requires ninein for spindle orientation and cortical microtubule organization
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441496/
https://www.ncbi.nlm.nih.gov/pubmed/30923192
http://dx.doi.org/10.26508/lsa.201900373
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