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First Description of Macrolide-Resistant Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia in Italy

BACKGROUND: Mycoplasma pneumoniae is a common cause of community-acquired pneumonia (CAP). This cross-sectional study aimed to determine the prevalence of macrolide-resistant M. pneumoniae strains in a convenience series of 234 adult hospitalised and nonhospitalised subjects with a diagnosis of CAP...

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Autores principales: Loconsole, Daniela, De Robertis, Anna Lisa, Mallamaci, Rosanna, Sallustio, Anna, Morea, Anna, Prato, Rosa, Quarto, Michele, Martinelli, Domenico, Chironna, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441498/
https://www.ncbi.nlm.nih.gov/pubmed/31008110
http://dx.doi.org/10.1155/2019/7168949
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author Loconsole, Daniela
De Robertis, Anna Lisa
Mallamaci, Rosanna
Sallustio, Anna
Morea, Anna
Prato, Rosa
Quarto, Michele
Martinelli, Domenico
Chironna, Maria
author_facet Loconsole, Daniela
De Robertis, Anna Lisa
Mallamaci, Rosanna
Sallustio, Anna
Morea, Anna
Prato, Rosa
Quarto, Michele
Martinelli, Domenico
Chironna, Maria
author_sort Loconsole, Daniela
collection PubMed
description BACKGROUND: Mycoplasma pneumoniae is a common cause of community-acquired pneumonia (CAP). This cross-sectional study aimed to determine the prevalence of macrolide-resistant M. pneumoniae strains in a convenience series of 234 adult hospitalised and nonhospitalised subjects with a diagnosis of CAP in January 2013 to April 2015 in South Italy. METHODS: Respiratory samples were subjected to real-time PCR. In M. pneumoniae-positive samples, domain V of 23S rRNA was sequenced to detect resistance-conferring point mutations. P1 major adhesion protein typing and multiple loci variable-number tandem repeat analysis (MLVA) were also performed. RESULTS: Of the 234 samples, 15 (6.4%) were positive for M. pneumoniae. Three of these had a macrolide-resistant genotype: two and one had A2063G and A2064G mutations, respectively. Fourteen of the 15 strains were subtyped: half had subtype 1 and half had subtype 2. Eight strains underwent MLVA profiling: one each had the J, A, and Z type. The remainder was unclassifiable. CONCLUSIONS: This novel discovery of macrolide-resistant M. pneumoniae strains in adults with CAP in Italy suggests that there may be increasing circulation of these strains in the population. To facilitate rapid optimization of the antibiotic strategy in Italy, macrolide resistance should be monitored by a surveillance system that is based on molecular methods.
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spelling pubmed-64414982019-04-21 First Description of Macrolide-Resistant Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia in Italy Loconsole, Daniela De Robertis, Anna Lisa Mallamaci, Rosanna Sallustio, Anna Morea, Anna Prato, Rosa Quarto, Michele Martinelli, Domenico Chironna, Maria Biomed Res Int Research Article BACKGROUND: Mycoplasma pneumoniae is a common cause of community-acquired pneumonia (CAP). This cross-sectional study aimed to determine the prevalence of macrolide-resistant M. pneumoniae strains in a convenience series of 234 adult hospitalised and nonhospitalised subjects with a diagnosis of CAP in January 2013 to April 2015 in South Italy. METHODS: Respiratory samples were subjected to real-time PCR. In M. pneumoniae-positive samples, domain V of 23S rRNA was sequenced to detect resistance-conferring point mutations. P1 major adhesion protein typing and multiple loci variable-number tandem repeat analysis (MLVA) were also performed. RESULTS: Of the 234 samples, 15 (6.4%) were positive for M. pneumoniae. Three of these had a macrolide-resistant genotype: two and one had A2063G and A2064G mutations, respectively. Fourteen of the 15 strains were subtyped: half had subtype 1 and half had subtype 2. Eight strains underwent MLVA profiling: one each had the J, A, and Z type. The remainder was unclassifiable. CONCLUSIONS: This novel discovery of macrolide-resistant M. pneumoniae strains in adults with CAP in Italy suggests that there may be increasing circulation of these strains in the population. To facilitate rapid optimization of the antibiotic strategy in Italy, macrolide resistance should be monitored by a surveillance system that is based on molecular methods. Hindawi 2019-03-17 /pmc/articles/PMC6441498/ /pubmed/31008110 http://dx.doi.org/10.1155/2019/7168949 Text en Copyright © 2019 Daniela Loconsole et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Loconsole, Daniela
De Robertis, Anna Lisa
Mallamaci, Rosanna
Sallustio, Anna
Morea, Anna
Prato, Rosa
Quarto, Michele
Martinelli, Domenico
Chironna, Maria
First Description of Macrolide-Resistant Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia in Italy
title First Description of Macrolide-Resistant Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia in Italy
title_full First Description of Macrolide-Resistant Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia in Italy
title_fullStr First Description of Macrolide-Resistant Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia in Italy
title_full_unstemmed First Description of Macrolide-Resistant Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia in Italy
title_short First Description of Macrolide-Resistant Mycoplasma pneumoniae in Adults with Community-Acquired Pneumonia in Italy
title_sort first description of macrolide-resistant mycoplasma pneumoniae in adults with community-acquired pneumonia in italy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441498/
https://www.ncbi.nlm.nih.gov/pubmed/31008110
http://dx.doi.org/10.1155/2019/7168949
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