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Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica)
Apple cultivars with durable resistance are needed for sustainable management of fire blight, the most destructive bacterial disease of apples. Although studies have identified genetic resistance to fire blight in both wild species and cultivated apples, more research is needed to understand the mol...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441656/ https://www.ncbi.nlm.nih.gov/pubmed/30962933 http://dx.doi.org/10.1038/s41438-019-0120-z |
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author | Silva, Katchen Julliany Pereira Singh, Jugpreet Bednarek, Ryland Fei, Zhangjun Khan, Awais |
author_facet | Silva, Katchen Julliany Pereira Singh, Jugpreet Bednarek, Ryland Fei, Zhangjun Khan, Awais |
author_sort | Silva, Katchen Julliany Pereira |
collection | PubMed |
description | Apple cultivars with durable resistance are needed for sustainable management of fire blight, the most destructive bacterial disease of apples. Although studies have identified genetic resistance to fire blight in both wild species and cultivated apples, more research is needed to understand the molecular mechanisms underlying host–pathogen interaction and differential genotypic responses to fire blight infection. We have analyzed phenotypic and transcriptional responses of ‘Empire’ and ‘Gala’ apple cultivars to fire blight by infecting them with a highly aggressive E. amylovora strain. Disease progress, based on the percentage of visual shoot necrosis, started showing significant (p < 0.001) differences between ‘Empire’ and ‘Gala’ 4 days after infection (dai). ‘Empire’ seems to slow down bacterial progress more rapidly after this point. We further compared transcriptome profiles of ‘Empire’ and ‘Gala’ at three different time points after fire blight infection. More genes showed differential expression in ‘Gala’ at earlier stages, but the number of differentially expressed genes increased in ‘Empire’ at 3 dai. Functional classes related to defense, cell cycle, response to stress, and biotic stress were identified and a few co-expression gene networks showed particular enrichment for plant defense and abiotic stress response genes. Several of these genes also co-localized in previously identified quantitative trait locus regions for fire blight resistance on linkage groups 7 and 12, and can serve as functional candidates for future research. These results highlight different molecular mechanisms for pathogen perception and control in two apple cultivars and will contribute toward better understanding of E. amylovora-Malus pathosystem. |
format | Online Article Text |
id | pubmed-6441656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-64416562019-04-08 Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica) Silva, Katchen Julliany Pereira Singh, Jugpreet Bednarek, Ryland Fei, Zhangjun Khan, Awais Hortic Res Article Apple cultivars with durable resistance are needed for sustainable management of fire blight, the most destructive bacterial disease of apples. Although studies have identified genetic resistance to fire blight in both wild species and cultivated apples, more research is needed to understand the molecular mechanisms underlying host–pathogen interaction and differential genotypic responses to fire blight infection. We have analyzed phenotypic and transcriptional responses of ‘Empire’ and ‘Gala’ apple cultivars to fire blight by infecting them with a highly aggressive E. amylovora strain. Disease progress, based on the percentage of visual shoot necrosis, started showing significant (p < 0.001) differences between ‘Empire’ and ‘Gala’ 4 days after infection (dai). ‘Empire’ seems to slow down bacterial progress more rapidly after this point. We further compared transcriptome profiles of ‘Empire’ and ‘Gala’ at three different time points after fire blight infection. More genes showed differential expression in ‘Gala’ at earlier stages, but the number of differentially expressed genes increased in ‘Empire’ at 3 dai. Functional classes related to defense, cell cycle, response to stress, and biotic stress were identified and a few co-expression gene networks showed particular enrichment for plant defense and abiotic stress response genes. Several of these genes also co-localized in previously identified quantitative trait locus regions for fire blight resistance on linkage groups 7 and 12, and can serve as functional candidates for future research. These results highlight different molecular mechanisms for pathogen perception and control in two apple cultivars and will contribute toward better understanding of E. amylovora-Malus pathosystem. Nature Publishing Group UK 2019-04-06 /pmc/articles/PMC6441656/ /pubmed/30962933 http://dx.doi.org/10.1038/s41438-019-0120-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Silva, Katchen Julliany Pereira Singh, Jugpreet Bednarek, Ryland Fei, Zhangjun Khan, Awais Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica) |
title | Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica) |
title_full | Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica) |
title_fullStr | Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica) |
title_full_unstemmed | Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica) |
title_short | Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica) |
title_sort | differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (malus × domestica) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441656/ https://www.ncbi.nlm.nih.gov/pubmed/30962933 http://dx.doi.org/10.1038/s41438-019-0120-z |
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