Cargando…

Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica)

Apple cultivars with durable resistance are needed for sustainable management of fire blight, the most destructive bacterial disease of apples. Although studies have identified genetic resistance to fire blight in both wild species and cultivated apples, more research is needed to understand the mol...

Descripción completa

Detalles Bibliográficos
Autores principales: Silva, Katchen Julliany Pereira, Singh, Jugpreet, Bednarek, Ryland, Fei, Zhangjun, Khan, Awais
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441656/
https://www.ncbi.nlm.nih.gov/pubmed/30962933
http://dx.doi.org/10.1038/s41438-019-0120-z
_version_ 1783407577613205504
author Silva, Katchen Julliany Pereira
Singh, Jugpreet
Bednarek, Ryland
Fei, Zhangjun
Khan, Awais
author_facet Silva, Katchen Julliany Pereira
Singh, Jugpreet
Bednarek, Ryland
Fei, Zhangjun
Khan, Awais
author_sort Silva, Katchen Julliany Pereira
collection PubMed
description Apple cultivars with durable resistance are needed for sustainable management of fire blight, the most destructive bacterial disease of apples. Although studies have identified genetic resistance to fire blight in both wild species and cultivated apples, more research is needed to understand the molecular mechanisms underlying host–pathogen interaction and differential genotypic responses to fire blight infection. We have analyzed phenotypic and transcriptional responses of ‘Empire’ and ‘Gala’ apple cultivars to fire blight by infecting them with a highly aggressive E. amylovora strain. Disease progress, based on the percentage of visual shoot necrosis, started showing significant (p < 0.001) differences between ‘Empire’ and ‘Gala’ 4 days after infection (dai). ‘Empire’ seems to slow down bacterial progress more rapidly after this point. We further compared transcriptome profiles of ‘Empire’ and ‘Gala’ at three different time points after fire blight infection. More genes showed differential expression in ‘Gala’ at earlier stages, but the number of differentially expressed genes increased in ‘Empire’ at 3 dai. Functional classes related to defense, cell cycle, response to stress, and biotic stress were identified and a few co-expression gene networks showed particular enrichment for plant defense and abiotic stress response genes. Several of these genes also co-localized in previously identified quantitative trait locus regions for fire blight resistance on linkage groups 7 and 12, and can serve as functional candidates for future research. These results highlight different molecular mechanisms for pathogen perception and control in two apple cultivars and will contribute toward better understanding of E. amylovora-Malus pathosystem.
format Online
Article
Text
id pubmed-6441656
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-64416562019-04-08 Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica) Silva, Katchen Julliany Pereira Singh, Jugpreet Bednarek, Ryland Fei, Zhangjun Khan, Awais Hortic Res Article Apple cultivars with durable resistance are needed for sustainable management of fire blight, the most destructive bacterial disease of apples. Although studies have identified genetic resistance to fire blight in both wild species and cultivated apples, more research is needed to understand the molecular mechanisms underlying host–pathogen interaction and differential genotypic responses to fire blight infection. We have analyzed phenotypic and transcriptional responses of ‘Empire’ and ‘Gala’ apple cultivars to fire blight by infecting them with a highly aggressive E. amylovora strain. Disease progress, based on the percentage of visual shoot necrosis, started showing significant (p < 0.001) differences between ‘Empire’ and ‘Gala’ 4 days after infection (dai). ‘Empire’ seems to slow down bacterial progress more rapidly after this point. We further compared transcriptome profiles of ‘Empire’ and ‘Gala’ at three different time points after fire blight infection. More genes showed differential expression in ‘Gala’ at earlier stages, but the number of differentially expressed genes increased in ‘Empire’ at 3 dai. Functional classes related to defense, cell cycle, response to stress, and biotic stress were identified and a few co-expression gene networks showed particular enrichment for plant defense and abiotic stress response genes. Several of these genes also co-localized in previously identified quantitative trait locus regions for fire blight resistance on linkage groups 7 and 12, and can serve as functional candidates for future research. These results highlight different molecular mechanisms for pathogen perception and control in two apple cultivars and will contribute toward better understanding of E. amylovora-Malus pathosystem. Nature Publishing Group UK 2019-04-06 /pmc/articles/PMC6441656/ /pubmed/30962933 http://dx.doi.org/10.1038/s41438-019-0120-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Silva, Katchen Julliany Pereira
Singh, Jugpreet
Bednarek, Ryland
Fei, Zhangjun
Khan, Awais
Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica)
title Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica)
title_full Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica)
title_fullStr Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica)
title_full_unstemmed Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica)
title_short Differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (Malus × domestica)
title_sort differential gene regulatory pathways and co-expression networks associated with fire blight infection in apple (malus × domestica)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441656/
https://www.ncbi.nlm.nih.gov/pubmed/30962933
http://dx.doi.org/10.1038/s41438-019-0120-z
work_keys_str_mv AT silvakatchenjullianypereira differentialgeneregulatorypathwaysandcoexpressionnetworksassociatedwithfireblightinfectioninapplemalusdomestica
AT singhjugpreet differentialgeneregulatorypathwaysandcoexpressionnetworksassociatedwithfireblightinfectioninapplemalusdomestica
AT bednarekryland differentialgeneregulatorypathwaysandcoexpressionnetworksassociatedwithfireblightinfectioninapplemalusdomestica
AT feizhangjun differentialgeneregulatorypathwaysandcoexpressionnetworksassociatedwithfireblightinfectioninapplemalusdomestica
AT khanawais differentialgeneregulatorypathwaysandcoexpressionnetworksassociatedwithfireblightinfectioninapplemalusdomestica