Data on the effect of heat and other technical variables on the detection of microRNAs in human serum

Data are presented on the number and levels of 384 microRNAs (miRNAs) quantified by reverse-transcription real-time quantitative polymerase chain reaction (RT-qPCR) in human serum analyzed under different experimental conditions. The technical variables tested were 1) heating of the serum samples at...

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Detalles Bibliográficos
Autores principales: Camacho, Luísa, Porter-Gill, Patricia, Silva, Camila S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441724/
https://www.ncbi.nlm.nih.gov/pubmed/30976632
http://dx.doi.org/10.1016/j.dib.2019.103750
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author Camacho, Luísa
Porter-Gill, Patricia
Silva, Camila S.
author_facet Camacho, Luísa
Porter-Gill, Patricia
Silva, Camila S.
author_sort Camacho, Luísa
collection PubMed
description Data are presented on the number and levels of 384 microRNAs (miRNAs) quantified by reverse-transcription real-time quantitative polymerase chain reaction (RT-qPCR) in human serum analyzed under different experimental conditions. The technical variables tested were 1) heating of the serum samples at 60 °C for 120 minutes prior to RNA extraction versus no heating; 2) RNA extraction using an Exiqon miRCURY RNA Isolation kit for Biofluids versus a Systems Biosciences SeraMir Exosome RNA Purification kit; 3) miRNA quantitation by RT-qPCR using an Exiqon SYBR Green Human Panel I versus an Applied Biosystems TaqMan Human microRNA Array A.
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spelling pubmed-64417242019-04-11 Data on the effect of heat and other technical variables on the detection of microRNAs in human serum Camacho, Luísa Porter-Gill, Patricia Silva, Camila S. Data Brief Biochemistry, Genetics and Molecular Biology Data are presented on the number and levels of 384 microRNAs (miRNAs) quantified by reverse-transcription real-time quantitative polymerase chain reaction (RT-qPCR) in human serum analyzed under different experimental conditions. The technical variables tested were 1) heating of the serum samples at 60 °C for 120 minutes prior to RNA extraction versus no heating; 2) RNA extraction using an Exiqon miRCURY RNA Isolation kit for Biofluids versus a Systems Biosciences SeraMir Exosome RNA Purification kit; 3) miRNA quantitation by RT-qPCR using an Exiqon SYBR Green Human Panel I versus an Applied Biosystems TaqMan Human microRNA Array A. Elsevier 2019-03-07 /pmc/articles/PMC6441724/ /pubmed/30976632 http://dx.doi.org/10.1016/j.dib.2019.103750 Text en http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Camacho, Luísa
Porter-Gill, Patricia
Silva, Camila S.
Data on the effect of heat and other technical variables on the detection of microRNAs in human serum
title Data on the effect of heat and other technical variables on the detection of microRNAs in human serum
title_full Data on the effect of heat and other technical variables on the detection of microRNAs in human serum
title_fullStr Data on the effect of heat and other technical variables on the detection of microRNAs in human serum
title_full_unstemmed Data on the effect of heat and other technical variables on the detection of microRNAs in human serum
title_short Data on the effect of heat and other technical variables on the detection of microRNAs in human serum
title_sort data on the effect of heat and other technical variables on the detection of micrornas in human serum
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441724/
https://www.ncbi.nlm.nih.gov/pubmed/30976632
http://dx.doi.org/10.1016/j.dib.2019.103750
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