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The influence of parasitism by Trypanosoma cruzi in the hematological parameters of the white ear opossum (Didelphis albiventris) from Campo Grande, Mato Grosso do Sul, Brazil

Considered ecologically generalist, Didelphis albiventris is reported as reservoir for different species of parasites, especially Trypanosoma cruzi. However, the knowledge about the influence of T. cruzi on hematological parameters of free-living opossum remains scarce. The present study aimed to ev...

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Autores principales: Arruda Gimenes Nantes, Wesley, Teixeira Gomes Barreto, Wanessa, Martins Santos, Filipe, Carvalho de Macedo, Gabriel, Castro Rucco, Andreza, de Oliveira Assis, William, Edith de Oliveira Porfírio, Grasiela, Braziliano de Andrade, Gisele, Maria Jansen, Ana, Miraglia Herrera, Heitor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441743/
https://www.ncbi.nlm.nih.gov/pubmed/30976512
http://dx.doi.org/10.1016/j.ijppaw.2019.03.015
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author Arruda Gimenes Nantes, Wesley
Teixeira Gomes Barreto, Wanessa
Martins Santos, Filipe
Carvalho de Macedo, Gabriel
Castro Rucco, Andreza
de Oliveira Assis, William
Edith de Oliveira Porfírio, Grasiela
Braziliano de Andrade, Gisele
Maria Jansen, Ana
Miraglia Herrera, Heitor
author_facet Arruda Gimenes Nantes, Wesley
Teixeira Gomes Barreto, Wanessa
Martins Santos, Filipe
Carvalho de Macedo, Gabriel
Castro Rucco, Andreza
de Oliveira Assis, William
Edith de Oliveira Porfírio, Grasiela
Braziliano de Andrade, Gisele
Maria Jansen, Ana
Miraglia Herrera, Heitor
author_sort Arruda Gimenes Nantes, Wesley
collection PubMed
description Considered ecologically generalist, Didelphis albiventris is reported as reservoir for different species of parasites, especially Trypanosoma cruzi. However, the knowledge about the influence of T. cruzi on hematological parameters of free-living opossum remains scarce. The present study aimed to evaluate the influence of T. cruzi on hematological parameters of white-ear opossums (D. albiventris) from Campo Grande, Mato Grosso do Sul, Brazil. The blood samples and biometric data were collected from 40 opossums captured by Tomahawk and Sherman traps in six urban forest fragments located in the city. The health of these animals was inferred, mainly, by means of blood parameters (PCV, RBC, WBC, MCV and WBC differential). Molecular detection of T. cruzi infection was performed by nested polymerase chain reaction (nPCR), using 18S and 24Sα rDNA region as target. Paired-t-test and simple linear regression were used for statistical analysis. No significant difference was observed between the averages of hematological variables in relation to gender and body condition. The molecular diagnosis showed that 32.5% (13/40) of the opossums were infected by T. cruzi, which presented lymphocytosis (3.4 ± 1.5) and eosinophilia (0.09 ± 0.13). Path analysis showed that T. cruzi infection resulted in increased numbers of lymphocytes and indirectly decreased the body condition of opossums. Moreover T. cruzi infection resulted in a direct effect on decrease of MCV. Overall, our results suggest that T. cruzi infection may represent a risk to health of opossums since the lymphocytosis may cause a secondary damage on body condition of infected animals.
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spelling pubmed-64417432019-04-11 The influence of parasitism by Trypanosoma cruzi in the hematological parameters of the white ear opossum (Didelphis albiventris) from Campo Grande, Mato Grosso do Sul, Brazil Arruda Gimenes Nantes, Wesley Teixeira Gomes Barreto, Wanessa Martins Santos, Filipe Carvalho de Macedo, Gabriel Castro Rucco, Andreza de Oliveira Assis, William Edith de Oliveira Porfírio, Grasiela Braziliano de Andrade, Gisele Maria Jansen, Ana Miraglia Herrera, Heitor Int J Parasitol Parasites Wildl Regular article Considered ecologically generalist, Didelphis albiventris is reported as reservoir for different species of parasites, especially Trypanosoma cruzi. However, the knowledge about the influence of T. cruzi on hematological parameters of free-living opossum remains scarce. The present study aimed to evaluate the influence of T. cruzi on hematological parameters of white-ear opossums (D. albiventris) from Campo Grande, Mato Grosso do Sul, Brazil. The blood samples and biometric data were collected from 40 opossums captured by Tomahawk and Sherman traps in six urban forest fragments located in the city. The health of these animals was inferred, mainly, by means of blood parameters (PCV, RBC, WBC, MCV and WBC differential). Molecular detection of T. cruzi infection was performed by nested polymerase chain reaction (nPCR), using 18S and 24Sα rDNA region as target. Paired-t-test and simple linear regression were used for statistical analysis. No significant difference was observed between the averages of hematological variables in relation to gender and body condition. The molecular diagnosis showed that 32.5% (13/40) of the opossums were infected by T. cruzi, which presented lymphocytosis (3.4 ± 1.5) and eosinophilia (0.09 ± 0.13). Path analysis showed that T. cruzi infection resulted in increased numbers of lymphocytes and indirectly decreased the body condition of opossums. Moreover T. cruzi infection resulted in a direct effect on decrease of MCV. Overall, our results suggest that T. cruzi infection may represent a risk to health of opossums since the lymphocytosis may cause a secondary damage on body condition of infected animals. Elsevier 2019-03-24 /pmc/articles/PMC6441743/ /pubmed/30976512 http://dx.doi.org/10.1016/j.ijppaw.2019.03.015 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Regular article
Arruda Gimenes Nantes, Wesley
Teixeira Gomes Barreto, Wanessa
Martins Santos, Filipe
Carvalho de Macedo, Gabriel
Castro Rucco, Andreza
de Oliveira Assis, William
Edith de Oliveira Porfírio, Grasiela
Braziliano de Andrade, Gisele
Maria Jansen, Ana
Miraglia Herrera, Heitor
The influence of parasitism by Trypanosoma cruzi in the hematological parameters of the white ear opossum (Didelphis albiventris) from Campo Grande, Mato Grosso do Sul, Brazil
title The influence of parasitism by Trypanosoma cruzi in the hematological parameters of the white ear opossum (Didelphis albiventris) from Campo Grande, Mato Grosso do Sul, Brazil
title_full The influence of parasitism by Trypanosoma cruzi in the hematological parameters of the white ear opossum (Didelphis albiventris) from Campo Grande, Mato Grosso do Sul, Brazil
title_fullStr The influence of parasitism by Trypanosoma cruzi in the hematological parameters of the white ear opossum (Didelphis albiventris) from Campo Grande, Mato Grosso do Sul, Brazil
title_full_unstemmed The influence of parasitism by Trypanosoma cruzi in the hematological parameters of the white ear opossum (Didelphis albiventris) from Campo Grande, Mato Grosso do Sul, Brazil
title_short The influence of parasitism by Trypanosoma cruzi in the hematological parameters of the white ear opossum (Didelphis albiventris) from Campo Grande, Mato Grosso do Sul, Brazil
title_sort influence of parasitism by trypanosoma cruzi in the hematological parameters of the white ear opossum (didelphis albiventris) from campo grande, mato grosso do sul, brazil
topic Regular article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441743/
https://www.ncbi.nlm.nih.gov/pubmed/30976512
http://dx.doi.org/10.1016/j.ijppaw.2019.03.015
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