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Genetic Characterization of Resistance to Pyrenophora teres f. teres in the International Barley Differential Canadian Lake Shore
Genetic resistance to net form of net blotch in the international barley differential Canadian Lake Shore (CLS) was characterized and mapped. A doubled haploid (DH) population generated from a cross between CLS and susceptible cultivar Harrington was evaluated at the seedling stage using eight diver...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442539/ https://www.ncbi.nlm.nih.gov/pubmed/30967885 http://dx.doi.org/10.3389/fpls.2019.00326 |
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author | Dinglasan, Eric Hickey, Lee Ziems, Laura Fowler, Ryan Anisimova, Anna Baranova, Olga Lashina, Nina Afanasenko, Olga |
author_facet | Dinglasan, Eric Hickey, Lee Ziems, Laura Fowler, Ryan Anisimova, Anna Baranova, Olga Lashina, Nina Afanasenko, Olga |
author_sort | Dinglasan, Eric |
collection | PubMed |
description | Genetic resistance to net form of net blotch in the international barley differential Canadian Lake Shore (CLS) was characterized and mapped. A doubled haploid (DH) population generated from a cross between CLS and susceptible cultivar Harrington was evaluated at the seedling stage using eight diverse Pyrenophora teres f. teres (Ptt) isolates and at the adult stage in the field using natural inoculum. To effectively map the CLS resistance, comparative marker frequency analysis (MFA) was performed using 8,762 polymorphic DArT-seq markers, where ‘resistant’ and ‘susceptible’ groups each comprised 40 DH lines displaying the most extreme phenotypes. Five DArTseq markers were consistently detected in eight disease assays, which was designated qPttCLS and deemed to harbor the locus underpinning CLS resistance. Four of these markers were present onto the barley DArTseq physical map and spans a region between 398203862 and 435526243 bp which were found to consist several genes involved in important plant functions such as disease response and signaling pathways. While MFA only detected the 3H region, genetic analyses based on segregation patterns were inconsistent, suggesting complex inheritance or variation in phenotypic expression of qPttCLS, particularly in the field. This study represents progress toward connecting Ptt pathotype surveys with the corresponding resistance genes in barley differentials. The markers associated with qPttCLS are useful for marker-assisted selection in breeding programs. |
format | Online Article Text |
id | pubmed-6442539 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64425392019-04-09 Genetic Characterization of Resistance to Pyrenophora teres f. teres in the International Barley Differential Canadian Lake Shore Dinglasan, Eric Hickey, Lee Ziems, Laura Fowler, Ryan Anisimova, Anna Baranova, Olga Lashina, Nina Afanasenko, Olga Front Plant Sci Plant Science Genetic resistance to net form of net blotch in the international barley differential Canadian Lake Shore (CLS) was characterized and mapped. A doubled haploid (DH) population generated from a cross between CLS and susceptible cultivar Harrington was evaluated at the seedling stage using eight diverse Pyrenophora teres f. teres (Ptt) isolates and at the adult stage in the field using natural inoculum. To effectively map the CLS resistance, comparative marker frequency analysis (MFA) was performed using 8,762 polymorphic DArT-seq markers, where ‘resistant’ and ‘susceptible’ groups each comprised 40 DH lines displaying the most extreme phenotypes. Five DArTseq markers were consistently detected in eight disease assays, which was designated qPttCLS and deemed to harbor the locus underpinning CLS resistance. Four of these markers were present onto the barley DArTseq physical map and spans a region between 398203862 and 435526243 bp which were found to consist several genes involved in important plant functions such as disease response and signaling pathways. While MFA only detected the 3H region, genetic analyses based on segregation patterns were inconsistent, suggesting complex inheritance or variation in phenotypic expression of qPttCLS, particularly in the field. This study represents progress toward connecting Ptt pathotype surveys with the corresponding resistance genes in barley differentials. The markers associated with qPttCLS are useful for marker-assisted selection in breeding programs. Frontiers Media S.A. 2019-03-25 /pmc/articles/PMC6442539/ /pubmed/30967885 http://dx.doi.org/10.3389/fpls.2019.00326 Text en Copyright © 2019 Dinglasan, Hickey, Ziems, Fowler, Anisimova, Baranova, Lashina and Afanasenko. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Dinglasan, Eric Hickey, Lee Ziems, Laura Fowler, Ryan Anisimova, Anna Baranova, Olga Lashina, Nina Afanasenko, Olga Genetic Characterization of Resistance to Pyrenophora teres f. teres in the International Barley Differential Canadian Lake Shore |
title | Genetic Characterization of Resistance to Pyrenophora teres f. teres in the International Barley Differential Canadian Lake Shore |
title_full | Genetic Characterization of Resistance to Pyrenophora teres f. teres in the International Barley Differential Canadian Lake Shore |
title_fullStr | Genetic Characterization of Resistance to Pyrenophora teres f. teres in the International Barley Differential Canadian Lake Shore |
title_full_unstemmed | Genetic Characterization of Resistance to Pyrenophora teres f. teres in the International Barley Differential Canadian Lake Shore |
title_short | Genetic Characterization of Resistance to Pyrenophora teres f. teres in the International Barley Differential Canadian Lake Shore |
title_sort | genetic characterization of resistance to pyrenophora teres f. teres in the international barley differential canadian lake shore |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442539/ https://www.ncbi.nlm.nih.gov/pubmed/30967885 http://dx.doi.org/10.3389/fpls.2019.00326 |
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