Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-strand...

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Autores principales: Ignatov, Konstantin B., Blagodatskikh, Konstantin A., Shcherbo, Dmitry S., Kramarova, Tatiana V., Monakhova, Yulia A., Kramarov, Vladimir M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443234/
https://www.ncbi.nlm.nih.gov/pubmed/30933980
http://dx.doi.org/10.1371/journal.pone.0210374
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author Ignatov, Konstantin B.
Blagodatskikh, Konstantin A.
Shcherbo, Dmitry S.
Kramarova, Tatiana V.
Monakhova, Yulia A.
Kramarov, Vladimir M.
author_facet Ignatov, Konstantin B.
Blagodatskikh, Konstantin A.
Shcherbo, Dmitry S.
Kramarova, Tatiana V.
Monakhova, Yulia A.
Kramarov, Vladimir M.
author_sort Ignatov, Konstantin B.
collection PubMed
description Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends.
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spelling pubmed-64432342019-04-17 Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing Ignatov, Konstantin B. Blagodatskikh, Konstantin A. Shcherbo, Dmitry S. Kramarova, Tatiana V. Monakhova, Yulia A. Kramarov, Vladimir M. PLoS One Research Article Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends. Public Library of Science 2019-04-01 /pmc/articles/PMC6443234/ /pubmed/30933980 http://dx.doi.org/10.1371/journal.pone.0210374 Text en © 2019 Ignatov et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ignatov, Konstantin B.
Blagodatskikh, Konstantin A.
Shcherbo, Dmitry S.
Kramarova, Tatiana V.
Monakhova, Yulia A.
Kramarov, Vladimir M.
Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing
title Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing
title_full Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing
title_fullStr Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing
title_full_unstemmed Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing
title_short Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing
title_sort fragmentation through polymerization (ftp): a new method to fragment dna for next-generation sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443234/
https://www.ncbi.nlm.nih.gov/pubmed/30933980
http://dx.doi.org/10.1371/journal.pone.0210374
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