SNAC-tag for Sequence-specific Chemical Protein Cleavage

Biocompatible chemical protein cleavage methods have been long-sought to replace enzymatic cleavages, but have yet to be realized. Here, we report the development of the SNAC-tag (Sequence-specific Nickel Assisted Cleavage) to achieve sequence-specific chemical protein cleavage under biocompatible c...

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Detalles Bibliográficos
Autores principales: Dang, Bobo, Mravic, Marco, Hu, Hailin, Schmidt, Nathan, Mensa, Bruk, DeGrado, William F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443254/
https://www.ncbi.nlm.nih.gov/pubmed/30923372
http://dx.doi.org/10.1038/s41592-019-0357-3
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author Dang, Bobo
Mravic, Marco
Hu, Hailin
Schmidt, Nathan
Mensa, Bruk
DeGrado, William F.
author_facet Dang, Bobo
Mravic, Marco
Hu, Hailin
Schmidt, Nathan
Mensa, Bruk
DeGrado, William F.
author_sort Dang, Bobo
collection PubMed
description Biocompatible chemical protein cleavage methods have been long-sought to replace enzymatic cleavages, but have yet to be realized. Here, we report the development of the SNAC-tag (Sequence-specific Nickel Assisted Cleavage) to achieve sequence-specific chemical protein cleavage under biocompatible conditions with comparable efficiency to enzymes. We demonstrate that the SNAC-tag can be inserted before both water-soluble and membrane proteins to achieve fusion protein cleavage, even when enzymatic cleavages fail.
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spelling pubmed-64432542019-09-25 SNAC-tag for Sequence-specific Chemical Protein Cleavage Dang, Bobo Mravic, Marco Hu, Hailin Schmidt, Nathan Mensa, Bruk DeGrado, William F. Nat Methods Article Biocompatible chemical protein cleavage methods have been long-sought to replace enzymatic cleavages, but have yet to be realized. Here, we report the development of the SNAC-tag (Sequence-specific Nickel Assisted Cleavage) to achieve sequence-specific chemical protein cleavage under biocompatible conditions with comparable efficiency to enzymes. We demonstrate that the SNAC-tag can be inserted before both water-soluble and membrane proteins to achieve fusion protein cleavage, even when enzymatic cleavages fail. 2019-03-25 2019-04 /pmc/articles/PMC6443254/ /pubmed/30923372 http://dx.doi.org/10.1038/s41592-019-0357-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Dang, Bobo
Mravic, Marco
Hu, Hailin
Schmidt, Nathan
Mensa, Bruk
DeGrado, William F.
SNAC-tag for Sequence-specific Chemical Protein Cleavage
title SNAC-tag for Sequence-specific Chemical Protein Cleavage
title_full SNAC-tag for Sequence-specific Chemical Protein Cleavage
title_fullStr SNAC-tag for Sequence-specific Chemical Protein Cleavage
title_full_unstemmed SNAC-tag for Sequence-specific Chemical Protein Cleavage
title_short SNAC-tag for Sequence-specific Chemical Protein Cleavage
title_sort snac-tag for sequence-specific chemical protein cleavage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443254/
https://www.ncbi.nlm.nih.gov/pubmed/30923372
http://dx.doi.org/10.1038/s41592-019-0357-3
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AT schmidtnathan snactagforsequencespecificchemicalproteincleavage
AT mensabruk snactagforsequencespecificchemicalproteincleavage
AT degradowilliamf snactagforsequencespecificchemicalproteincleavage

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