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SETDB1 regulates SMAD7 expression for breast cancer metastasis

Breast cancer (BRC) is the most invasive cancer in women. Although the survival rate of BRC is gradually increasing due to improved screening systems, development of novel therapeutic targets for inhibition of BRC proliferation, metastasis and recurrence have been constantly needed. Thus, in this st...

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Autores principales: Ryu, Tae Young, Kim, Kwangho, Kim, Seon-Kyu, Oh, Jung-Hwa, Min, Jeong-Ki, Jung, Cho-Rok, Son, Mi-Young, Kim, Dae-Soo, Cho, Hyun-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443319/
https://www.ncbi.nlm.nih.gov/pubmed/30545440
http://dx.doi.org/10.5483/BMBRep.2019.52.2.235
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author Ryu, Tae Young
Kim, Kwangho
Kim, Seon-Kyu
Oh, Jung-Hwa
Min, Jeong-Ki
Jung, Cho-Rok
Son, Mi-Young
Kim, Dae-Soo
Cho, Hyun-Soo
author_facet Ryu, Tae Young
Kim, Kwangho
Kim, Seon-Kyu
Oh, Jung-Hwa
Min, Jeong-Ki
Jung, Cho-Rok
Son, Mi-Young
Kim, Dae-Soo
Cho, Hyun-Soo
author_sort Ryu, Tae Young
collection PubMed
description Breast cancer (BRC) is the most invasive cancer in women. Although the survival rate of BRC is gradually increasing due to improved screening systems, development of novel therapeutic targets for inhibition of BRC proliferation, metastasis and recurrence have been constantly needed. Thus, in this study, we identified overexpression of SETDB1 (SET Domain Bifurcated 1), a histone methyltransferase, in RNA-seq data of BRC derived from TCGA portal. In Gene Ontology (GO) analysis, cell migration-related GO terms were enriched, and we confirmed down-regulation of cell migration/invasion and alteration of EMT/MET markers after knockdown of SETDB1. Moreover, gene network analysis showed that SMAD7 expression is regulated by SETDB1 levels, indicating that up-regulation of SMAD7 by SETDB1 knockdown inhibited BRC metastasis. Therefore, development of SETDB1 inhibitors and functional studies may help develop more effective clinical guidelines for BRC treatment.
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spelling pubmed-64433192019-04-15 SETDB1 regulates SMAD7 expression for breast cancer metastasis Ryu, Tae Young Kim, Kwangho Kim, Seon-Kyu Oh, Jung-Hwa Min, Jeong-Ki Jung, Cho-Rok Son, Mi-Young Kim, Dae-Soo Cho, Hyun-Soo BMB Rep Articles Breast cancer (BRC) is the most invasive cancer in women. Although the survival rate of BRC is gradually increasing due to improved screening systems, development of novel therapeutic targets for inhibition of BRC proliferation, metastasis and recurrence have been constantly needed. Thus, in this study, we identified overexpression of SETDB1 (SET Domain Bifurcated 1), a histone methyltransferase, in RNA-seq data of BRC derived from TCGA portal. In Gene Ontology (GO) analysis, cell migration-related GO terms were enriched, and we confirmed down-regulation of cell migration/invasion and alteration of EMT/MET markers after knockdown of SETDB1. Moreover, gene network analysis showed that SMAD7 expression is regulated by SETDB1 levels, indicating that up-regulation of SMAD7 by SETDB1 knockdown inhibited BRC metastasis. Therefore, development of SETDB1 inhibitors and functional studies may help develop more effective clinical guidelines for BRC treatment. Korean Society for Biochemistry and Molecular Biology 2019-02 2019-02-28 /pmc/articles/PMC6443319/ /pubmed/30545440 http://dx.doi.org/10.5483/BMBRep.2019.52.2.235 Text en Copyright © 2019 by the The Korean Society for Biochemistry and Molecular Biology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Ryu, Tae Young
Kim, Kwangho
Kim, Seon-Kyu
Oh, Jung-Hwa
Min, Jeong-Ki
Jung, Cho-Rok
Son, Mi-Young
Kim, Dae-Soo
Cho, Hyun-Soo
SETDB1 regulates SMAD7 expression for breast cancer metastasis
title SETDB1 regulates SMAD7 expression for breast cancer metastasis
title_full SETDB1 regulates SMAD7 expression for breast cancer metastasis
title_fullStr SETDB1 regulates SMAD7 expression for breast cancer metastasis
title_full_unstemmed SETDB1 regulates SMAD7 expression for breast cancer metastasis
title_short SETDB1 regulates SMAD7 expression for breast cancer metastasis
title_sort setdb1 regulates smad7 expression for breast cancer metastasis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443319/
https://www.ncbi.nlm.nih.gov/pubmed/30545440
http://dx.doi.org/10.5483/BMBRep.2019.52.2.235
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