Protein denaturation at the air-water interface and how to prevent it

Electron cryo-microscopy analyzes the structure of proteins and protein complexes in vitrified solution. Proteins tend to adsorb to the air-water interface in unsupported films of aqueous solution, which can result in partial or complete denaturation. We investigated the structure of yeast fatty aci...

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Detalles Bibliográficos
Autores principales: D'Imprima, Edoardo, Floris, Davide, Joppe, Mirko, Sánchez, Ricardo, Grininger, Martin, Kühlbrandt, Werner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Structural Biology and Molecular Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443348/
https://www.ncbi.nlm.nih.gov/pubmed/30932812
http://dx.doi.org/10.7554/eLife.42747

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443348/
https://www.ncbi.nlm.nih.gov/pubmed/30932812
http://dx.doi.org/10.7554/eLife.42747

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