Cargando…
NanoBRET: The Bright Future of Proximity-Based Assays
Bioluminescence resonance energy transfer (BRET) is a biophysical technique used to monitor proximity within live cells. BRET exploits the naturally occurring phenomenon of dipole-dipole energy transfer from a donor enzyme (luciferase) to an acceptor fluorophore following enzyme-mediated oxidation o...
Autores principales: | Dale, Natasha C., Johnstone, Elizabeth K. M., White, Carl W., Pfleger, Kevin D. G. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443706/ https://www.ncbi.nlm.nih.gov/pubmed/30972335 http://dx.doi.org/10.3389/fbioe.2019.00056 |
Ejemplares similares
-
Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time
por: White, Carl W., et al.
Publicado: (2017) -
Investigation of Receptor Heteromers Using NanoBRET Ligand Binding
por: Johnstone, Elizabeth K. M., et al.
Publicado: (2021) -
Experimental determination of the bioluminescence resonance energy transfer (BRET) Förster distances of NanoBRET and red-shifted BRET pairs
por: Weihs, Felix, et al.
Publicado: (2020) -
Evaluating steroid hormone receptor interactions using the live-cell NanoBRET proximity assay
por: Huggins, Rosemary J, et al.
Publicado: (2023) -
Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay
por: Spencer-Smith, Russell, et al.
Publicado: (2023)