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Exosomal tRNA-derived small RNA as a promising biomarker for cancer diagnosis
tRNA-derived small RNA (tsRNA) is a novel regulatory small non-coding RNA and participates in diverse physiological and pathological processes. However, the presence of tsRNAs in exosome and their diagnostic potential remain unclear. In this study, we took advantage of small RNA-seq technology to pr...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444574/ https://www.ncbi.nlm.nih.gov/pubmed/30940133 http://dx.doi.org/10.1186/s12943-019-1000-8 |
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author | Zhu, Lei Li, Jiao Gong, Youling Wu, Qingbin Tan, Shuangyan Sun, Dan Xu, Xiaomin Zuo, Yuanli Zhao, Yun Wei, Yu-Quan Wei, Xia-Wei Peng, Yong |
author_facet | Zhu, Lei Li, Jiao Gong, Youling Wu, Qingbin Tan, Shuangyan Sun, Dan Xu, Xiaomin Zuo, Yuanli Zhao, Yun Wei, Yu-Quan Wei, Xia-Wei Peng, Yong |
author_sort | Zhu, Lei |
collection | PubMed |
description | tRNA-derived small RNA (tsRNA) is a novel regulatory small non-coding RNA and participates in diverse physiological and pathological processes. However, the presence of tsRNAs in exosome and their diagnostic potential remain unclear. In this study, we took advantage of small RNA-seq technology to profile exosomal tsRNAs from cell culture medium and plasma, and found ubiquitous presence of tsRNAs in exosome. To explore the potential value of tsRNA for cancer diagnosis, we compared exosomal tsRNA levels between liver cancer patients and healthy donors, revealing that tsRNAs were dramatically increased in plasma exosomes of liver cancer patients. Importantly, patients with liver cancer exhibited significantly higher levels of four tsRNAs (tRNA-ValTAC-3, tRNA-GlyTCC-5, tRNA-ValAAC-5 and tRNA-GluCTC-5) in plasma exosome, demonstrating that plasma exosomal tsRNA could serve as a novel diagnostic biomarker. Taken together, our results not only expand non-coding RNA species in exosome, but also highlight the potential of tsRNAs as a promising biomarker for cancer diagnosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12943-019-1000-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6444574 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-64445742019-04-11 Exosomal tRNA-derived small RNA as a promising biomarker for cancer diagnosis Zhu, Lei Li, Jiao Gong, Youling Wu, Qingbin Tan, Shuangyan Sun, Dan Xu, Xiaomin Zuo, Yuanli Zhao, Yun Wei, Yu-Quan Wei, Xia-Wei Peng, Yong Mol Cancer Letter to the Editor tRNA-derived small RNA (tsRNA) is a novel regulatory small non-coding RNA and participates in diverse physiological and pathological processes. However, the presence of tsRNAs in exosome and their diagnostic potential remain unclear. In this study, we took advantage of small RNA-seq technology to profile exosomal tsRNAs from cell culture medium and plasma, and found ubiquitous presence of tsRNAs in exosome. To explore the potential value of tsRNA for cancer diagnosis, we compared exosomal tsRNA levels between liver cancer patients and healthy donors, revealing that tsRNAs were dramatically increased in plasma exosomes of liver cancer patients. Importantly, patients with liver cancer exhibited significantly higher levels of four tsRNAs (tRNA-ValTAC-3, tRNA-GlyTCC-5, tRNA-ValAAC-5 and tRNA-GluCTC-5) in plasma exosome, demonstrating that plasma exosomal tsRNA could serve as a novel diagnostic biomarker. Taken together, our results not only expand non-coding RNA species in exosome, but also highlight the potential of tsRNAs as a promising biomarker for cancer diagnosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12943-019-1000-8) contains supplementary material, which is available to authorized users. BioMed Central 2019-04-02 /pmc/articles/PMC6444574/ /pubmed/30940133 http://dx.doi.org/10.1186/s12943-019-1000-8 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Letter to the Editor Zhu, Lei Li, Jiao Gong, Youling Wu, Qingbin Tan, Shuangyan Sun, Dan Xu, Xiaomin Zuo, Yuanli Zhao, Yun Wei, Yu-Quan Wei, Xia-Wei Peng, Yong Exosomal tRNA-derived small RNA as a promising biomarker for cancer diagnosis |
title | Exosomal tRNA-derived small RNA as a promising biomarker for cancer diagnosis |
title_full | Exosomal tRNA-derived small RNA as a promising biomarker for cancer diagnosis |
title_fullStr | Exosomal tRNA-derived small RNA as a promising biomarker for cancer diagnosis |
title_full_unstemmed | Exosomal tRNA-derived small RNA as a promising biomarker for cancer diagnosis |
title_short | Exosomal tRNA-derived small RNA as a promising biomarker for cancer diagnosis |
title_sort | exosomal trna-derived small rna as a promising biomarker for cancer diagnosis |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444574/ https://www.ncbi.nlm.nih.gov/pubmed/30940133 http://dx.doi.org/10.1186/s12943-019-1000-8 |
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