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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utility of CRISPR-based technologies. Current approaches based on tRNA processing offer a promising strategy but suffer from high background. Here, to address this limitation, we present a screening platform which al...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445147/ https://www.ncbi.nlm.nih.gov/pubmed/30940799 http://dx.doi.org/10.1038/s41467-019-09148-3 |