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Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages

PURPOSE: The use of bacteriophages represents a valid alternative to conventional antimicrobial treatments, overcoming the widespread bacterial antibiotic resistance phenomenon. In this work, we evaluated whether biomimetic hydroxyapatite (HA) nanocrystals are able to enhance some properties of bact...

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Autores principales: Fulgione, Andrea, Ianniello, Flora, Papaianni, Marina, Contaldi, Felice, Sgamma, Tiziana, Giannini, Cinzia, Pastore, Stella, Velotta, Raffaele, Della Ventura, Bartolomeo, Roveri, Norberto, Lelli, Marco, Capuano, Federico, Capparelli, Rosanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445186/
https://www.ncbi.nlm.nih.gov/pubmed/30992664
http://dx.doi.org/10.2147/IJN.S190188
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author Fulgione, Andrea
Ianniello, Flora
Papaianni, Marina
Contaldi, Felice
Sgamma, Tiziana
Giannini, Cinzia
Pastore, Stella
Velotta, Raffaele
Della Ventura, Bartolomeo
Roveri, Norberto
Lelli, Marco
Capuano, Federico
Capparelli, Rosanna
author_facet Fulgione, Andrea
Ianniello, Flora
Papaianni, Marina
Contaldi, Felice
Sgamma, Tiziana
Giannini, Cinzia
Pastore, Stella
Velotta, Raffaele
Della Ventura, Bartolomeo
Roveri, Norberto
Lelli, Marco
Capuano, Federico
Capparelli, Rosanna
author_sort Fulgione, Andrea
collection PubMed
description PURPOSE: The use of bacteriophages represents a valid alternative to conventional antimicrobial treatments, overcoming the widespread bacterial antibiotic resistance phenomenon. In this work, we evaluated whether biomimetic hydroxyapatite (HA) nanocrystals are able to enhance some properties of bacteriophages. The final goal of this study was to demonstrate that biomimetic HA nanocrystals can be used for bacteriophage delivery in the context of bacterial infections, and contribute – at the same time – to enhance some of the biological properties of the same bacteriophages such as stability, preservation, antimicrobial activity, and so on. MATERIALS AND METHODS: Phage isolation and characterization were carried out by using Mitomycin C and following double-layer agar technique. The biomimetic HA water suspension was synthesized in order to obtain nanocrystals with plate-like morphology and nanometric dimensions. The interaction of phages with the HA was investigated by dynamic light scattering and Zeta potential analyses. The cytotoxicity and intracellular killing activities of the phage–HA complex were evaluated in human hepatocellular carcinoma HepG2 cells. The bacterial inhibition capacity of the complex was assessed on chicken minced meat samples infected with Salmonella Rissen. RESULTS: Our data highlighted that the biomimetic HA nanocrystal–bacteriophage complex was more stable and more effective than phages alone in all tested experimental conditions. CONCLUSION: Our results evidenced the important contribution of biomimetic HA nanocrystals: they act as an excellent carrier for bacteriophage delivery and enhance its biological characteristics. This study confirmed the significant role of the mineral HA when it is complexed with biological entities like bacteriophages, as it has been shown for molecules such as lactoferrin.
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spelling pubmed-64451862019-04-16 Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages Fulgione, Andrea Ianniello, Flora Papaianni, Marina Contaldi, Felice Sgamma, Tiziana Giannini, Cinzia Pastore, Stella Velotta, Raffaele Della Ventura, Bartolomeo Roveri, Norberto Lelli, Marco Capuano, Federico Capparelli, Rosanna Int J Nanomedicine Original Research PURPOSE: The use of bacteriophages represents a valid alternative to conventional antimicrobial treatments, overcoming the widespread bacterial antibiotic resistance phenomenon. In this work, we evaluated whether biomimetic hydroxyapatite (HA) nanocrystals are able to enhance some properties of bacteriophages. The final goal of this study was to demonstrate that biomimetic HA nanocrystals can be used for bacteriophage delivery in the context of bacterial infections, and contribute – at the same time – to enhance some of the biological properties of the same bacteriophages such as stability, preservation, antimicrobial activity, and so on. MATERIALS AND METHODS: Phage isolation and characterization were carried out by using Mitomycin C and following double-layer agar technique. The biomimetic HA water suspension was synthesized in order to obtain nanocrystals with plate-like morphology and nanometric dimensions. The interaction of phages with the HA was investigated by dynamic light scattering and Zeta potential analyses. The cytotoxicity and intracellular killing activities of the phage–HA complex were evaluated in human hepatocellular carcinoma HepG2 cells. The bacterial inhibition capacity of the complex was assessed on chicken minced meat samples infected with Salmonella Rissen. RESULTS: Our data highlighted that the biomimetic HA nanocrystal–bacteriophage complex was more stable and more effective than phages alone in all tested experimental conditions. CONCLUSION: Our results evidenced the important contribution of biomimetic HA nanocrystals: they act as an excellent carrier for bacteriophage delivery and enhance its biological characteristics. This study confirmed the significant role of the mineral HA when it is complexed with biological entities like bacteriophages, as it has been shown for molecules such as lactoferrin. Dove Medical Press 2019-03-28 /pmc/articles/PMC6445186/ /pubmed/30992664 http://dx.doi.org/10.2147/IJN.S190188 Text en © 2019 Fulgione et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Fulgione, Andrea
Ianniello, Flora
Papaianni, Marina
Contaldi, Felice
Sgamma, Tiziana
Giannini, Cinzia
Pastore, Stella
Velotta, Raffaele
Della Ventura, Bartolomeo
Roveri, Norberto
Lelli, Marco
Capuano, Federico
Capparelli, Rosanna
Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages
title Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages
title_full Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages
title_fullStr Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages
title_full_unstemmed Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages
title_short Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages
title_sort biomimetic hydroxyapatite nanocrystals are an active carrier for salmonella bacteriophages
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445186/
https://www.ncbi.nlm.nih.gov/pubmed/30992664
http://dx.doi.org/10.2147/IJN.S190188
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