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Fetal lung C4BPA induces p100 processing in human placenta

The non-canonical NF-κB signaling may be a central integrator of a placental clock that governs the length of human pregnancy. We sought to identify fetal signals that could activate this NF-κB pathway in the placenta, and in turn, contribute to the onset of labor. Proteomics analysis of exosomes pu...

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Autores principales: Ithier, Mayra Cruz, Parobchak, Nataliya, Yadava, Stacy, Cheng, Jerry, Wang, Bingbing, Rosen, Todd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445281/
https://www.ncbi.nlm.nih.gov/pubmed/30940885
http://dx.doi.org/10.1038/s41598-019-42078-0
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author Ithier, Mayra Cruz
Parobchak, Nataliya
Yadava, Stacy
Cheng, Jerry
Wang, Bingbing
Rosen, Todd
author_facet Ithier, Mayra Cruz
Parobchak, Nataliya
Yadava, Stacy
Cheng, Jerry
Wang, Bingbing
Rosen, Todd
author_sort Ithier, Mayra Cruz
collection PubMed
description The non-canonical NF-κB signaling may be a central integrator of a placental clock that governs the length of human pregnancy. We sought to identify fetal signals that could activate this NF-κB pathway in the placenta, and in turn, contribute to the onset of labor. Proteomics analysis of exosomes purified from fetal cord arterial blood revealed a total of 328 proteins, among which 48 were more significantly abundant (p < 0.01) in samples from women who delivered following elective Cesarean-section at term (39 to 40 weeks of estimated gestational age, EGA) compared to those who had elective Cesarean deliveries near term (35 to 36 weeks of EGA). Computational, crystal structural, and gene functional analyses showed that one of these 48 proteins, C4BPA, binds to CD40 of placental villous trophoblast to activate p100 processing to p52, and in turn, pro-labor genes. These results suggest that fetal C4BPA-induced activation of non-canonical NF-κB in human placenta may play a critical role in processes of term or preterm labor.
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spelling pubmed-64452812019-04-05 Fetal lung C4BPA induces p100 processing in human placenta Ithier, Mayra Cruz Parobchak, Nataliya Yadava, Stacy Cheng, Jerry Wang, Bingbing Rosen, Todd Sci Rep Article The non-canonical NF-κB signaling may be a central integrator of a placental clock that governs the length of human pregnancy. We sought to identify fetal signals that could activate this NF-κB pathway in the placenta, and in turn, contribute to the onset of labor. Proteomics analysis of exosomes purified from fetal cord arterial blood revealed a total of 328 proteins, among which 48 were more significantly abundant (p < 0.01) in samples from women who delivered following elective Cesarean-section at term (39 to 40 weeks of estimated gestational age, EGA) compared to those who had elective Cesarean deliveries near term (35 to 36 weeks of EGA). Computational, crystal structural, and gene functional analyses showed that one of these 48 proteins, C4BPA, binds to CD40 of placental villous trophoblast to activate p100 processing to p52, and in turn, pro-labor genes. These results suggest that fetal C4BPA-induced activation of non-canonical NF-κB in human placenta may play a critical role in processes of term or preterm labor. Nature Publishing Group UK 2019-04-02 /pmc/articles/PMC6445281/ /pubmed/30940885 http://dx.doi.org/10.1038/s41598-019-42078-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ithier, Mayra Cruz
Parobchak, Nataliya
Yadava, Stacy
Cheng, Jerry
Wang, Bingbing
Rosen, Todd
Fetal lung C4BPA induces p100 processing in human placenta
title Fetal lung C4BPA induces p100 processing in human placenta
title_full Fetal lung C4BPA induces p100 processing in human placenta
title_fullStr Fetal lung C4BPA induces p100 processing in human placenta
title_full_unstemmed Fetal lung C4BPA induces p100 processing in human placenta
title_short Fetal lung C4BPA induces p100 processing in human placenta
title_sort fetal lung c4bpa induces p100 processing in human placenta
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445281/
https://www.ncbi.nlm.nih.gov/pubmed/30940885
http://dx.doi.org/10.1038/s41598-019-42078-0
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