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Prokaryotic community shifts during soil formation on sands in the tundra zone

A chronosequence approach, i.e., a comparison of spatially distinct plots with different stages of succession, is commonly used for studying microbial community dynamics during paedogenesis. The successional traits of prokaryotic communities following sand fixation processes have previously been cha...

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Autores principales: Zhelezova, Alena, Chernov, Timofey, Tkhakakhova, Azida, Xenofontova, Natalya, Semenov, Mikhail, Kutovaya, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445424/
https://www.ncbi.nlm.nih.gov/pubmed/30939175
http://dx.doi.org/10.1371/journal.pone.0206777
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author Zhelezova, Alena
Chernov, Timofey
Tkhakakhova, Azida
Xenofontova, Natalya
Semenov, Mikhail
Kutovaya, Olga
author_facet Zhelezova, Alena
Chernov, Timofey
Tkhakakhova, Azida
Xenofontova, Natalya
Semenov, Mikhail
Kutovaya, Olga
author_sort Zhelezova, Alena
collection PubMed
description A chronosequence approach, i.e., a comparison of spatially distinct plots with different stages of succession, is commonly used for studying microbial community dynamics during paedogenesis. The successional traits of prokaryotic communities following sand fixation processes have previously been characterized for arid and semi-arid regions, but they have not been considered for the tundra zone, where the environmental conditions are unfavourable for the establishment of complicated biocoenoses. In this research, we characterized the prokaryotic diversity and abundance of microbial genes found in a typical tundra and wooded tundra along a gradient of increasing vegetation—unfixed aeolian sand, semi-fixed surfaces with mosses and lichens, and mature soil under fully developed plant cover. Microbial communities from typical tundra and wooded tundra plots at three stages of sand fixation were compared using quantitative polymerase chain reaction (qPCR) and high-throughput sequencing of 16S rRNA gene libraries. The abundances of ribosomal genes increased gradually in both chronosequences, and a similar trend was observed for the functional genes related to the nitrogen cycle (nifH, bacterial amoA, nirK and nirS). The relative abundance of Planctomycetes increased, while those of Thaumarchaeota, Cyanobacteria and Chloroflexi decreased from unfixed sands to mature soils. According to β-diversity analysis, prokaryotic communities of unfixed sands were more heterogeneous compared to those of mature soils. Despite the differences in the plant cover of the two mature soils, the structural compositions of the prokaryotic communities were shaped in the same way. Thus, sand fixation in the tundra zone increases archaeal, bacterial and fungal abundances, shifts and unifies prokaryotic communities structure.
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spelling pubmed-64454242019-04-17 Prokaryotic community shifts during soil formation on sands in the tundra zone Zhelezova, Alena Chernov, Timofey Tkhakakhova, Azida Xenofontova, Natalya Semenov, Mikhail Kutovaya, Olga PLoS One Research Article A chronosequence approach, i.e., a comparison of spatially distinct plots with different stages of succession, is commonly used for studying microbial community dynamics during paedogenesis. The successional traits of prokaryotic communities following sand fixation processes have previously been characterized for arid and semi-arid regions, but they have not been considered for the tundra zone, where the environmental conditions are unfavourable for the establishment of complicated biocoenoses. In this research, we characterized the prokaryotic diversity and abundance of microbial genes found in a typical tundra and wooded tundra along a gradient of increasing vegetation—unfixed aeolian sand, semi-fixed surfaces with mosses and lichens, and mature soil under fully developed plant cover. Microbial communities from typical tundra and wooded tundra plots at three stages of sand fixation were compared using quantitative polymerase chain reaction (qPCR) and high-throughput sequencing of 16S rRNA gene libraries. The abundances of ribosomal genes increased gradually in both chronosequences, and a similar trend was observed for the functional genes related to the nitrogen cycle (nifH, bacterial amoA, nirK and nirS). The relative abundance of Planctomycetes increased, while those of Thaumarchaeota, Cyanobacteria and Chloroflexi decreased from unfixed sands to mature soils. According to β-diversity analysis, prokaryotic communities of unfixed sands were more heterogeneous compared to those of mature soils. Despite the differences in the plant cover of the two mature soils, the structural compositions of the prokaryotic communities were shaped in the same way. Thus, sand fixation in the tundra zone increases archaeal, bacterial and fungal abundances, shifts and unifies prokaryotic communities structure. Public Library of Science 2019-04-02 /pmc/articles/PMC6445424/ /pubmed/30939175 http://dx.doi.org/10.1371/journal.pone.0206777 Text en © 2019 Zhelezova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhelezova, Alena
Chernov, Timofey
Tkhakakhova, Azida
Xenofontova, Natalya
Semenov, Mikhail
Kutovaya, Olga
Prokaryotic community shifts during soil formation on sands in the tundra zone
title Prokaryotic community shifts during soil formation on sands in the tundra zone
title_full Prokaryotic community shifts during soil formation on sands in the tundra zone
title_fullStr Prokaryotic community shifts during soil formation on sands in the tundra zone
title_full_unstemmed Prokaryotic community shifts during soil formation on sands in the tundra zone
title_short Prokaryotic community shifts during soil formation on sands in the tundra zone
title_sort prokaryotic community shifts during soil formation on sands in the tundra zone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445424/
https://www.ncbi.nlm.nih.gov/pubmed/30939175
http://dx.doi.org/10.1371/journal.pone.0206777
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