NRT1.1 Regulates Nitrate Allocation and Cadmium Tolerance in Arabidopsis

Abiotic stress induces nitrate (NO(3)(-)) allocation to roots, which increases stress tolerance in plants. NRT1.1 is broadly involved in abiotic stress tolerance in plants, but the relationship between NRT1.1 and NO(3)(-) allocation under stress conditions is unclear. In this study, we found that Arabidopsis wild-type Col-0 was more cadmium (Cd(2+))-tolerant than the nrt1.1 mutant at 20 μM CdCl(2). Cd(2+) exposure repressed NRT1.5 but upregulated NRT1.8 in roots of Col-0 plants, resulting in increased NO(3)(-) allocation to roots and higher [NO(3)(-)] root-to-shoot (R:S) ratios. Interestingly, NITRATE REGULATORY GENE2 (NRG2) was upregulated by Cd(2+) stress in Col-0 but not in nrt1.1. Under Cd(2+) stress, nrg2 and nrg2-3chl1-13 mutants exhibited similar phenotypes and NO(3)(-) allocation patterns as observed in the nrt1.1 mutant, but overexpression of NRG2 in Col-0 and nrt1.1 increased the [NO(3)(-)] R:S ratio and restored Cd(2+) stress tolerance. Our results indicated that NRT1.1 and NRG2 regulated Cd(2+) stress-induced NO(3)(-) allocation to roots and that NRG2 functioned downstream of NRT1.1. Cd(2+) uptake did not differ between Col-0 and nrt1.1, but Cd(2+) allocation to roots was higher in Col-0 than in nrt1.1. Stressed Col-0 plants increased Cd(2+) and NO(3)(-) allocation to root vacuoles, which reduced their cytosolic allocation and transport to the shoots. Our results suggest that NRT1.1 regulates NO(3)(-) allocation to roots by coordinating Cd(2+) accumulation in root vacuoles, which facilitates Cd(2+) detoxification.