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Aminated Graphene Oxide as a Potential New Therapy for Colorectal Cancer

Nanotechnology-based drug delivery systems for cancer therapy are the topic of interest for many researchers and scientists. Graphene oxide (GO) and its derivates are among the most extensively studied delivery systems of this type. The increased surface area, elevated loading capacity, and aptitude...

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Autores principales: Krasteva, Natalia, Keremidarska-Markova, Milena, Hristova-Panusheva, Kamelia, Andreeva, Tonya, Speranza, Giorgio, Wang, Dayong, Draganova-Filipova, Milena, Miloshev, George, Georgieva, Milena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446092/
https://www.ncbi.nlm.nih.gov/pubmed/31015889
http://dx.doi.org/10.1155/2019/3738980
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author Krasteva, Natalia
Keremidarska-Markova, Milena
Hristova-Panusheva, Kamelia
Andreeva, Tonya
Speranza, Giorgio
Wang, Dayong
Draganova-Filipova, Milena
Miloshev, George
Georgieva, Milena
author_facet Krasteva, Natalia
Keremidarska-Markova, Milena
Hristova-Panusheva, Kamelia
Andreeva, Tonya
Speranza, Giorgio
Wang, Dayong
Draganova-Filipova, Milena
Miloshev, George
Georgieva, Milena
author_sort Krasteva, Natalia
collection PubMed
description Nanotechnology-based drug delivery systems for cancer therapy are the topic of interest for many researchers and scientists. Graphene oxide (GO) and its derivates are among the most extensively studied delivery systems of this type. The increased surface area, elevated loading capacity, and aptitude for surface functionalization together with the ability to induce reactive oxygen species make GO a promising tool for the development of novel anticancer therapies. Moreover, GO nanoparticles not only function as effective drug carriers but also have the potential to exert their own inhibitory effects on tumour cells. Recent results show that the functionalization of GO with different functional groups, namely, with amine groups, leads to increased reactivity of the nanoparticles. The last steers different hypotheses for the mechanisms through which this functionalization of GO could potentially lead to improved anticancer capacity. In this research, we have evaluated the potential of amine-functionalized graphene oxide nanoparticles (GO-NH(2)) as new molecules for colorectal cancer therapy. For the purpose, we have assessed the impact of aminated graphene oxide (GO) sheets on the viability of colon cancer cells, their potential to generate ROS, and their potential to influence cellular proliferation and survival. In order to elucidate their mechanism of action on the cellular systems, we have probed their genotoxic and cytostatic properties and compared them to pristine GO. Our results revealed that both GO samples (pristine and aminated) were composed of few-layer sheets with different particle sizes, zeta potential, and surface characteristics. Furthermore, we have detected increased cyto- and genotoxicity of the aminated GO nanoparticles following 24-hour exposure on Colon 26 cells. The last leads us to conclude that exposure of cancer cells to GO, namely, aminated GO, can significantly contribute to cancer cell killing by enhancing the cytotoxicity effect exerted through the induction of ROS, subsequent DNA damage, and apoptosis.
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spelling pubmed-64460922019-04-23 Aminated Graphene Oxide as a Potential New Therapy for Colorectal Cancer Krasteva, Natalia Keremidarska-Markova, Milena Hristova-Panusheva, Kamelia Andreeva, Tonya Speranza, Giorgio Wang, Dayong Draganova-Filipova, Milena Miloshev, George Georgieva, Milena Oxid Med Cell Longev Research Article Nanotechnology-based drug delivery systems for cancer therapy are the topic of interest for many researchers and scientists. Graphene oxide (GO) and its derivates are among the most extensively studied delivery systems of this type. The increased surface area, elevated loading capacity, and aptitude for surface functionalization together with the ability to induce reactive oxygen species make GO a promising tool for the development of novel anticancer therapies. Moreover, GO nanoparticles not only function as effective drug carriers but also have the potential to exert their own inhibitory effects on tumour cells. Recent results show that the functionalization of GO with different functional groups, namely, with amine groups, leads to increased reactivity of the nanoparticles. The last steers different hypotheses for the mechanisms through which this functionalization of GO could potentially lead to improved anticancer capacity. In this research, we have evaluated the potential of amine-functionalized graphene oxide nanoparticles (GO-NH(2)) as new molecules for colorectal cancer therapy. For the purpose, we have assessed the impact of aminated graphene oxide (GO) sheets on the viability of colon cancer cells, their potential to generate ROS, and their potential to influence cellular proliferation and survival. In order to elucidate their mechanism of action on the cellular systems, we have probed their genotoxic and cytostatic properties and compared them to pristine GO. Our results revealed that both GO samples (pristine and aminated) were composed of few-layer sheets with different particle sizes, zeta potential, and surface characteristics. Furthermore, we have detected increased cyto- and genotoxicity of the aminated GO nanoparticles following 24-hour exposure on Colon 26 cells. The last leads us to conclude that exposure of cancer cells to GO, namely, aminated GO, can significantly contribute to cancer cell killing by enhancing the cytotoxicity effect exerted through the induction of ROS, subsequent DNA damage, and apoptosis. Hindawi 2019-03-20 /pmc/articles/PMC6446092/ /pubmed/31015889 http://dx.doi.org/10.1155/2019/3738980 Text en Copyright © 2019 Natalia Krasteva et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Krasteva, Natalia
Keremidarska-Markova, Milena
Hristova-Panusheva, Kamelia
Andreeva, Tonya
Speranza, Giorgio
Wang, Dayong
Draganova-Filipova, Milena
Miloshev, George
Georgieva, Milena
Aminated Graphene Oxide as a Potential New Therapy for Colorectal Cancer
title Aminated Graphene Oxide as a Potential New Therapy for Colorectal Cancer
title_full Aminated Graphene Oxide as a Potential New Therapy for Colorectal Cancer
title_fullStr Aminated Graphene Oxide as a Potential New Therapy for Colorectal Cancer
title_full_unstemmed Aminated Graphene Oxide as a Potential New Therapy for Colorectal Cancer
title_short Aminated Graphene Oxide as a Potential New Therapy for Colorectal Cancer
title_sort aminated graphene oxide as a potential new therapy for colorectal cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446092/
https://www.ncbi.nlm.nih.gov/pubmed/31015889
http://dx.doi.org/10.1155/2019/3738980
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