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Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake

Even though cell walls have essential functions for bacteria, fungi, and plants, tools to investigate their dynamic structure in living cells have been missing. Here, it is shown that changes in the intensity of the plasma membrane dye FM4-64 in response to extracellular quenchers depend on the nano...

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Autores principales: Liu, Xiaohui, Li, Jiazhou, Zhao, Heyu, Liu, Boyang, Günther-Pomorski, Thomas, Chen, Shaolin, Liesche, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446840/
https://www.ncbi.nlm.nih.gov/pubmed/30782779
http://dx.doi.org/10.1083/jcb.201810121
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author Liu, Xiaohui
Li, Jiazhou
Zhao, Heyu
Liu, Boyang
Günther-Pomorski, Thomas
Chen, Shaolin
Liesche, Johannes
author_facet Liu, Xiaohui
Li, Jiazhou
Zhao, Heyu
Liu, Boyang
Günther-Pomorski, Thomas
Chen, Shaolin
Liesche, Johannes
author_sort Liu, Xiaohui
collection PubMed
description Even though cell walls have essential functions for bacteria, fungi, and plants, tools to investigate their dynamic structure in living cells have been missing. Here, it is shown that changes in the intensity of the plasma membrane dye FM4-64 in response to extracellular quenchers depend on the nano-scale porosity of cell walls. The correlation of quenching efficiency and cell wall porosity is supported by tests on various cell types, application of differently sized quenchers, and comparison of results with confocal, electron, and atomic force microscopy images. The quenching assay was used to investigate how changes in cell wall porosity affect the capability for extension growth in the model plant Arabidopsis thaliana. Results suggest that increased porosity is not a precondition but a result of cell extension, thereby providing new insight on the mechanism plant organ growth. Furthermore, it was shown that higher cell wall porosity can facilitate the action of antifungal drugs in Saccharomyces cerevisiae, presumably by facilitating uptake.
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spelling pubmed-64468402019-10-01 Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake Liu, Xiaohui Li, Jiazhou Zhao, Heyu Liu, Boyang Günther-Pomorski, Thomas Chen, Shaolin Liesche, Johannes J Cell Biol Research Articles Even though cell walls have essential functions for bacteria, fungi, and plants, tools to investigate their dynamic structure in living cells have been missing. Here, it is shown that changes in the intensity of the plasma membrane dye FM4-64 in response to extracellular quenchers depend on the nano-scale porosity of cell walls. The correlation of quenching efficiency and cell wall porosity is supported by tests on various cell types, application of differently sized quenchers, and comparison of results with confocal, electron, and atomic force microscopy images. The quenching assay was used to investigate how changes in cell wall porosity affect the capability for extension growth in the model plant Arabidopsis thaliana. Results suggest that increased porosity is not a precondition but a result of cell extension, thereby providing new insight on the mechanism plant organ growth. Furthermore, it was shown that higher cell wall porosity can facilitate the action of antifungal drugs in Saccharomyces cerevisiae, presumably by facilitating uptake. Rockefeller University Press 2019-04-01 2019-02-19 /pmc/articles/PMC6446840/ /pubmed/30782779 http://dx.doi.org/10.1083/jcb.201810121 Text en © 2019 Liu et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Liu, Xiaohui
Li, Jiazhou
Zhao, Heyu
Liu, Boyang
Günther-Pomorski, Thomas
Chen, Shaolin
Liesche, Johannes
Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake
title Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake
title_full Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake
title_fullStr Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake
title_full_unstemmed Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake
title_short Novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake
title_sort novel tool to quantify cell wall porosity relates wall structure to cell growth and drug uptake
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446840/
https://www.ncbi.nlm.nih.gov/pubmed/30782779
http://dx.doi.org/10.1083/jcb.201810121
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