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Biomarkers for PVR in rhegmatogenous retinal detachment
PURPOSE: Various profibrotic and proinflammatory cytokines have been found upregulated in uncomplicated primary retinal detachment (pRD), but without providing a uniform picture. Here, we compare the cyto- and chemokine profiles in pRD with and without proliferative vitreoretinopathy (PVR) in an att...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447182/ https://www.ncbi.nlm.nih.gov/pubmed/30943234 http://dx.doi.org/10.1371/journal.pone.0214674 |
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author | Zandi, Souska Pfister, Isabel B. Traine, Peter G. Tappeiner, Christoph Despont, Alain Rieben, Robert Skowronska, Magdalena Garweg, Justus G. |
author_facet | Zandi, Souska Pfister, Isabel B. Traine, Peter G. Tappeiner, Christoph Despont, Alain Rieben, Robert Skowronska, Magdalena Garweg, Justus G. |
author_sort | Zandi, Souska |
collection | PubMed |
description | PURPOSE: Various profibrotic and proinflammatory cytokines have been found upregulated in uncomplicated primary retinal detachment (pRD), but without providing a uniform picture. Here, we compare the cyto- and chemokine profiles in pRD with and without proliferative vitreoretinopathy (PVR) in an attempt to unravel relevant differences not in single cytokines, but in the cytokine profiles at diagnosis. METHODS: Undiluted vitreous fluid (VF) was obtained at the beginning of surgery from 174 eyes with pRD without relevant PVR (maximally grade B; group 1; n = 81) and with moderate or advanced PVR requiring a gas tamponade (group 2; n = 49) or silicon oil filling (group 3; n = 44). VF of eyes undergoing macular hole (MH) surgery served as controls (group 4; n = 26). Forty-three cytokines were quantified in parallel using a multiplex cytokine analysis system (Bioplex). For all comparisons we applied Holm’s correction to control for multiple comparisons. RESULTS: 44.9% of group 2 eyes presented grade C1 and 55.1% C2-C3, whereas 86.4% of group 3 eyes exhibited a PVR grade of C2-D. CCL19 was the only cytokine that displayed higher concentrations in the vitreous of eyes with PVR C1 compared to lower PVR grades. Eyes with PVR C2-D showed higher levels of CCL27, CXCL6, IL4, IL16, CXCL10, CCL8, CCL22, MIG/CXCL9, CCL15, CCL19, CCL 23 and CXCL12 compared to controls. Interestingly, no difference of cytokine levels was detected between C1 and C2-D PVR. CONCLUSIONS: CCL19 may represent a potential biomarker for early PVR progression that holds promise for future diagnostic and therapeutic applications. |
format | Online Article Text |
id | pubmed-6447182 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64471822019-04-17 Biomarkers for PVR in rhegmatogenous retinal detachment Zandi, Souska Pfister, Isabel B. Traine, Peter G. Tappeiner, Christoph Despont, Alain Rieben, Robert Skowronska, Magdalena Garweg, Justus G. PLoS One Research Article PURPOSE: Various profibrotic and proinflammatory cytokines have been found upregulated in uncomplicated primary retinal detachment (pRD), but without providing a uniform picture. Here, we compare the cyto- and chemokine profiles in pRD with and without proliferative vitreoretinopathy (PVR) in an attempt to unravel relevant differences not in single cytokines, but in the cytokine profiles at diagnosis. METHODS: Undiluted vitreous fluid (VF) was obtained at the beginning of surgery from 174 eyes with pRD without relevant PVR (maximally grade B; group 1; n = 81) and with moderate or advanced PVR requiring a gas tamponade (group 2; n = 49) or silicon oil filling (group 3; n = 44). VF of eyes undergoing macular hole (MH) surgery served as controls (group 4; n = 26). Forty-three cytokines were quantified in parallel using a multiplex cytokine analysis system (Bioplex). For all comparisons we applied Holm’s correction to control for multiple comparisons. RESULTS: 44.9% of group 2 eyes presented grade C1 and 55.1% C2-C3, whereas 86.4% of group 3 eyes exhibited a PVR grade of C2-D. CCL19 was the only cytokine that displayed higher concentrations in the vitreous of eyes with PVR C1 compared to lower PVR grades. Eyes with PVR C2-D showed higher levels of CCL27, CXCL6, IL4, IL16, CXCL10, CCL8, CCL22, MIG/CXCL9, CCL15, CCL19, CCL 23 and CXCL12 compared to controls. Interestingly, no difference of cytokine levels was detected between C1 and C2-D PVR. CONCLUSIONS: CCL19 may represent a potential biomarker for early PVR progression that holds promise for future diagnostic and therapeutic applications. Public Library of Science 2019-04-03 /pmc/articles/PMC6447182/ /pubmed/30943234 http://dx.doi.org/10.1371/journal.pone.0214674 Text en © 2019 Zandi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zandi, Souska Pfister, Isabel B. Traine, Peter G. Tappeiner, Christoph Despont, Alain Rieben, Robert Skowronska, Magdalena Garweg, Justus G. Biomarkers for PVR in rhegmatogenous retinal detachment |
title | Biomarkers for PVR in rhegmatogenous retinal detachment |
title_full | Biomarkers for PVR in rhegmatogenous retinal detachment |
title_fullStr | Biomarkers for PVR in rhegmatogenous retinal detachment |
title_full_unstemmed | Biomarkers for PVR in rhegmatogenous retinal detachment |
title_short | Biomarkers for PVR in rhegmatogenous retinal detachment |
title_sort | biomarkers for pvr in rhegmatogenous retinal detachment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447182/ https://www.ncbi.nlm.nih.gov/pubmed/30943234 http://dx.doi.org/10.1371/journal.pone.0214674 |
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