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Universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis
OBJECTIVES: Compare the molecular epidemiology of tuberculosis (TB) between two large Canadian provinces–Ontario and British Columbia (BC)–to identify genotypic clusters within and across both provinces, allowing for an improved understanding of genotype data and providing context to more accurately...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447219/ https://www.ncbi.nlm.nih.gov/pubmed/30943250 http://dx.doi.org/10.1371/journal.pone.0214870 |
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author | Guthrie, Jennifer L. Marchand-Austin, Alex Cronin, Kirby Lam, Karen Pyskir, Daria Kong, Clare Jorgensen, Danielle Rodrigues, Mabel Roth, David Tang, Patrick Cook, Victoria J. Johnston, James Jamieson, Frances B. Gardy, Jennifer L. |
author_facet | Guthrie, Jennifer L. Marchand-Austin, Alex Cronin, Kirby Lam, Karen Pyskir, Daria Kong, Clare Jorgensen, Danielle Rodrigues, Mabel Roth, David Tang, Patrick Cook, Victoria J. Johnston, James Jamieson, Frances B. Gardy, Jennifer L. |
author_sort | Guthrie, Jennifer L. |
collection | PubMed |
description | OBJECTIVES: Compare the molecular epidemiology of tuberculosis (TB) between two large Canadian provinces–Ontario and British Columbia (BC)–to identify genotypic clusters within and across both provinces, allowing for an improved understanding of genotype data and providing context to more accurately identify clusters representing local transmission. DESIGN: We compared 24-locus Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTR) genotyping for 3,314 Ontario and 1,602 BC clinical Mycobacterium tuberculosis isolates collected from 2008 through 2014. Laboratory data for each isolate was linked to case-level records to obtain clinical and demographic data. RESULTS: The demographic characteristics of persons with TB varied between provinces, most notably in the proportion of persons born outside Canada, which was reflected in the large number of unique genotypes (n = 3,461). The proportion of clustered isolates was significantly higher in BC. Substantial clustering amongst non-Lineage 4 TB strains was observed within and across the provinces. Only two large clusters (≥10 cases/cluster) representing within province transmission had interprovincial genotype matches. CONCLUSION: We recommend expanding analysis of shared genotypes to include neighbouring jurisdictions, and implementing whole genome sequencing to improve identification of TB transmission, recognize outbreaks, and monitor changing trends in TB epidemiology. |
format | Online Article Text |
id | pubmed-6447219 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64472192019-04-17 Universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis Guthrie, Jennifer L. Marchand-Austin, Alex Cronin, Kirby Lam, Karen Pyskir, Daria Kong, Clare Jorgensen, Danielle Rodrigues, Mabel Roth, David Tang, Patrick Cook, Victoria J. Johnston, James Jamieson, Frances B. Gardy, Jennifer L. PLoS One Research Article OBJECTIVES: Compare the molecular epidemiology of tuberculosis (TB) between two large Canadian provinces–Ontario and British Columbia (BC)–to identify genotypic clusters within and across both provinces, allowing for an improved understanding of genotype data and providing context to more accurately identify clusters representing local transmission. DESIGN: We compared 24-locus Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTR) genotyping for 3,314 Ontario and 1,602 BC clinical Mycobacterium tuberculosis isolates collected from 2008 through 2014. Laboratory data for each isolate was linked to case-level records to obtain clinical and demographic data. RESULTS: The demographic characteristics of persons with TB varied between provinces, most notably in the proportion of persons born outside Canada, which was reflected in the large number of unique genotypes (n = 3,461). The proportion of clustered isolates was significantly higher in BC. Substantial clustering amongst non-Lineage 4 TB strains was observed within and across the provinces. Only two large clusters (≥10 cases/cluster) representing within province transmission had interprovincial genotype matches. CONCLUSION: We recommend expanding analysis of shared genotypes to include neighbouring jurisdictions, and implementing whole genome sequencing to improve identification of TB transmission, recognize outbreaks, and monitor changing trends in TB epidemiology. Public Library of Science 2019-04-03 /pmc/articles/PMC6447219/ /pubmed/30943250 http://dx.doi.org/10.1371/journal.pone.0214870 Text en © 2019 Guthrie et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Guthrie, Jennifer L. Marchand-Austin, Alex Cronin, Kirby Lam, Karen Pyskir, Daria Kong, Clare Jorgensen, Danielle Rodrigues, Mabel Roth, David Tang, Patrick Cook, Victoria J. Johnston, James Jamieson, Frances B. Gardy, Jennifer L. Universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis |
title | Universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis |
title_full | Universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis |
title_fullStr | Universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis |
title_full_unstemmed | Universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis |
title_short | Universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis |
title_sort | universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447219/ https://www.ncbi.nlm.nih.gov/pubmed/30943250 http://dx.doi.org/10.1371/journal.pone.0214870 |
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