Cargando…

Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions

Technologies that convert transient protein-protein interactions (PPIs) into stable expression of a reporter gene are useful for genetic selections, high-throughput screening, and multiplexing with omics technologies. We previously reported SPARK (Kim et al., 2017), a transcription factor that is ac...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kim, Christina K, Cho, Kelvin F, Kim, Min Woo, Ting, Alice Y
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2019
Materias:	Cell Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447360/ https://www.ncbi.nlm.nih.gov/pubmed/30942168 http://dx.doi.org/10.7554/eLife.43826

Ejemplares similares

Time-gated detection of protein-protein interactions with transcriptional readout
por: Kim, Min Woo, et al.
Publicado: (2017)

Short LOV Proteins in Methylocystis Reveal Insight into LOV Domain Photocycle Mechanisms
por: El-Arab, Kaley K., et al.
Publicado: (2015)

Structure of a LOV protein in apo-state and implications for construction of LOV-based optical tools
por: Arinkin, Vladimir, et al.
Publicado: (2017)

Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc
por: Aper, Stijn J. A., et al.
Publicado: (2016)

Reporter Coxsackievirus A5 Expressing iLOV Fluorescent Protein or Luciferase Used for Rapid Neutralizing Assay in Cells and Living Imaging in Mice
por: Jin, Wei-Ping, et al.
Publicado: (2023)

Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc‐Based BRET Reporter in Combination with Firefly Luciferase
por: Taylor, Arthur, et al.
Publicado: (2018)

Monitoring cellular redox dynamics using newly developed BRET-based redox sensor proteins
por: Fu, Nae, et al.
Publicado: (2021)

Comment on “The Use of BRET to Study Receptor-Protein Interactions”
por: Jockers, Ralf
Publicado: (2014)

Probing the Electron Transfer between iLOV Protein and Ag Nanoparticles
por: Ran, Xia, et al.
Publicado: (2020)

Optimization of BRET saturation assays for robust and sensitive cytosolic protein–protein interaction studies
por: Besson, Benoit, et al.
Publicado: (2022)

BRET based dual-colour (visible/near-infrared) molecular imaging using a quantum dot/EGFP–luciferase conjugate
por: Tsuboi, Setsuko, et al.
Publicado: (2019)

Light-induced structural changes in a short light, oxygen, voltage (LOV) protein revealed by molecular dynamics simulations—implications for the understanding of LOV photoactivation
por: Bocola, Marco, et al.
Publicado: (2015)

Improved HaloTag Ligand Enables BRET Imaging With NanoLuc
por: Thirukkumaran, Ovia Margaret, et al.
Publicado: (2020)

Experimental determination of the bioluminescence resonance energy transfer (BRET) Förster distances of NanoBRET and red-shifted BRET pairs
por: Weihs, Felix, et al.
Publicado: (2020)

Express Your LOV: An Engineered Flavoprotein as a Reporter for Protein Expression and Purification
por: Gawthorne, Jayde A., et al.
Publicado: (2012)

Computational Investigation of Structural and Spectroscopic Properties of LOV-Based Proteins with Improved Fluorescence
por: Cardoso Ramos, Felipe, et al.
Publicado: (2021)

LOV thy neighbor: Mapping protein interactomes by genetically encodable photoproximity labeling
por: Xie, Xiao, et al.
Publicado: (2023)

LOV conquers (sm)All GTPases
por: Kosoff, Rachelle, et al.
Publicado: (2010)

Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein
por: Garcia-Marcos, Mikel, et al.
Publicado: (2020)

Fluorescence Imaging-Based High-Throughput Screening of Fast- and Slow-Cycling LOV Proteins
por: Kawano, Fuun, et al.
Publicado: (2013)

Bright Bioluminescent BRET Sensor Proteins for Measuring Intracellular Caspase Activity
por: den Hamer, Anniek, et al.
Publicado: (2017)

The unexpected versatility of ALP/Enigma family proteins
por: Fisher, Lucas A. B., et al.
Publicado: (2022)

A luminescent Nanoluc-GFP fusion protein enables readout of cellular pH in photosynthetic organisms
por: Nakamura, Shungo, et al.
Publicado: (2020)

A BRET-based assay reveals collagen–Hsp47 interaction dynamics in the endoplasmic reticulum and small-molecule inhibition of this interaction
por: Ito, Shinya, et al.
Publicado: (2019)

Rationally improving LOV domain–based photoswitches
por: Strickland, Devin, et al.
Publicado: (2010)

Factors That Control the Chemistry of the LOV Domain Photocycle
por: Zayner, Josiah P., et al.
Publicado: (2014)

LOV-based optogenetic devices: light-driven modules to impart photoregulated control of cellular signaling
por: Pudasaini, Ashutosh, et al.
Publicado: (2015)

Cellular Membranes, a Versatile Adaptive Composite Material
por: Lamparter, Lucas, et al.
Publicado: (2020)

Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor
por: Gligorovski, Vojislav, et al.
Publicado: (2023)

The Medical Wisdom of Bret Harte
por: Bragman, Louis J.
Publicado: (1934)

In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects
por: Sun, Sihuai, et al.
Publicado: (2016)

Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae
por: Endres, Stephan, et al.
Publicado: (2015)

Electron transfer pathways in a light, oxygen, voltage (LOV) protein devoid of the photoactive cysteine
por: Kopka, Benita, et al.
Publicado: (2017)

Revealing Hidden Conformational Space of LOV Protein VIVID Through Rigid Residue Scan Simulations
por: Zhou, Hongyu, et al.
Publicado: (2017)

Engineering the protein dynamics of an ancestral luciferase
por: Schenkmayerova, Andrea, et al.
Publicado: (2021)

Development of a Quantitative BRET Affinity Assay for Nucleic Acid-Protein Interactions
por: Vickers, Timothy A., et al.
Publicado: (2016)

Sharing the wealth: The versatility of proteins targeted to peroxisomes and other organelles
por: Bittner, Elena, et al.
Publicado: (2022)

Distinct Conformational Dynamics of Three G Protein-Coupled Receptors Measured Using FlAsH-BRET Biosensors
por: Bourque, Kyla, et al.
Publicado: (2017)

Photocontrolled Exposure of Pro‐apoptotic Peptide Sequences in LOV Proteins Modulates Bcl‐2 Family Interactions
por: Mart, Robert J., et al.
Publicado: (2015)

Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein
por: Röllen, Katrin, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_vclrverm83mghk6rt4lvibapl3