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A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR
Stool contains DNA shed from cells of the gastrointestinal (GI) tract and has great potential as a bio-specimen for non-invasive, nucleic acid-based detection of GI diseases. Whereas methods for studying faecal microbiome DNA are plentiful, there is a lack of well-characterised procedures for stabil...
Autores principales: | He, Kuang, Fujiwara, Hideaki, Zajac, Cynthia, Sandford, Erin, Reddy, Pavan, Choi, Sung Won, Tewari, Muneesh |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447604/ https://www.ncbi.nlm.nih.gov/pubmed/30944341 http://dx.doi.org/10.1038/s41598-019-41753-6 |
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