Cargando…
Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food
Loop-mediated isothermal amplification (LAMP) has gained wide popularity in the detection of Salmonella in foods owing to its simplicity, rapidity, and robustness. This multi-laboratory validation (MLV) study aimed to validate a Salmonella LAMP-based method against the United States Food and Drug Ad...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447708/ https://www.ncbi.nlm.nih.gov/pubmed/30984125 http://dx.doi.org/10.3389/fmicb.2019.00562 |
_version_ | 1783408552928346112 |
---|---|
author | Ge, Beilei Domesle, Kelly J. Yang, Qianru Hammack, Thomas S. Wang, Shizhen S. Deng, Xiaohong Hu, Lijun Zhang, Guodong Hu, Yuan Lai, Xiaokuang Chou, Kyson X. Dollete, Jan Ryan Hirneisen, Kirsten A. La, Sammie P. Richter, Richelle S. Rai, Diyo R. Yousefvand, Azadeh A. Park, Paul K. Wu, Cindy H. Eames, Tameji Kiang, David Sheng, Ju Wu, Dancia Hahn, Lori Ledger, Lisa Logie, Cynthia You, Qiu Slavic, Durda Cai, Hugh Ayers, Sherry L. Young, Shenia R. Pamboukian, Ruiqing |
author_facet | Ge, Beilei Domesle, Kelly J. Yang, Qianru Hammack, Thomas S. Wang, Shizhen S. Deng, Xiaohong Hu, Lijun Zhang, Guodong Hu, Yuan Lai, Xiaokuang Chou, Kyson X. Dollete, Jan Ryan Hirneisen, Kirsten A. La, Sammie P. Richter, Richelle S. Rai, Diyo R. Yousefvand, Azadeh A. Park, Paul K. Wu, Cindy H. Eames, Tameji Kiang, David Sheng, Ju Wu, Dancia Hahn, Lori Ledger, Lisa Logie, Cynthia You, Qiu Slavic, Durda Cai, Hugh Ayers, Sherry L. Young, Shenia R. Pamboukian, Ruiqing |
author_sort | Ge, Beilei |
collection | PubMed |
description | Loop-mediated isothermal amplification (LAMP) has gained wide popularity in the detection of Salmonella in foods owing to its simplicity, rapidity, and robustness. This multi-laboratory validation (MLV) study aimed to validate a Salmonella LAMP-based method against the United States Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) Chapter 5 Salmonella reference method in a representative animal food matrix (dry dog food). Fourteen independent collaborators from seven laboratories in the United States and Canada participated in the study. Each collaborator received two sets of 24 blind-coded dry dog food samples (eight uninoculated; eight inoculated at a low level, 0.65 MPN/25 g; and eight inoculated at a high level, 3.01 MPN/25 g) and initiated the testing on the same day. The MLV study used an unpaired design where different test portions were analyzed by the LAMP and BAM methods using different preenrichment protocols (buffered peptone water for LAMP and lactose broth for BAM). All LAMP samples were confirmed by culture using the BAM method. BAM samples were also tested by LAMP following lactose broth preenrichment (paired samples). Statistical analysis was carried out by the probability of detection (POD) per AOAC guidelines and by a random intercept logistic regression model. Overall, no significant differences in POD between the Salmonella LAMP and BAM methods were observed with either unpaired or paired samples, indicating the methods were comparable. LAMP testing following preenrichment in buffered peptone water or lactose broth also resulted in insignificant POD differences (P > 0.05). The MLV study strongly supports the utility and applicability of this rapid and reliable LAMP method in routine regulatory screening of Salmonella in animal food. |
format | Online Article Text |
id | pubmed-6447708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64477082019-04-12 Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food Ge, Beilei Domesle, Kelly J. Yang, Qianru Hammack, Thomas S. Wang, Shizhen S. Deng, Xiaohong Hu, Lijun Zhang, Guodong Hu, Yuan Lai, Xiaokuang Chou, Kyson X. Dollete, Jan Ryan Hirneisen, Kirsten A. La, Sammie P. Richter, Richelle S. Rai, Diyo R. Yousefvand, Azadeh A. Park, Paul K. Wu, Cindy H. Eames, Tameji Kiang, David Sheng, Ju Wu, Dancia Hahn, Lori Ledger, Lisa Logie, Cynthia You, Qiu Slavic, Durda Cai, Hugh Ayers, Sherry L. Young, Shenia R. Pamboukian, Ruiqing Front Microbiol Microbiology Loop-mediated isothermal amplification (LAMP) has gained wide popularity in the detection of Salmonella in foods owing to its simplicity, rapidity, and robustness. This multi-laboratory validation (MLV) study aimed to validate a Salmonella LAMP-based method against the United States Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) Chapter 5 Salmonella reference method in a representative animal food matrix (dry dog food). Fourteen independent collaborators from seven laboratories in the United States and Canada participated in the study. Each collaborator received two sets of 24 blind-coded dry dog food samples (eight uninoculated; eight inoculated at a low level, 0.65 MPN/25 g; and eight inoculated at a high level, 3.01 MPN/25 g) and initiated the testing on the same day. The MLV study used an unpaired design where different test portions were analyzed by the LAMP and BAM methods using different preenrichment protocols (buffered peptone water for LAMP and lactose broth for BAM). All LAMP samples were confirmed by culture using the BAM method. BAM samples were also tested by LAMP following lactose broth preenrichment (paired samples). Statistical analysis was carried out by the probability of detection (POD) per AOAC guidelines and by a random intercept logistic regression model. Overall, no significant differences in POD between the Salmonella LAMP and BAM methods were observed with either unpaired or paired samples, indicating the methods were comparable. LAMP testing following preenrichment in buffered peptone water or lactose broth also resulted in insignificant POD differences (P > 0.05). The MLV study strongly supports the utility and applicability of this rapid and reliable LAMP method in routine regulatory screening of Salmonella in animal food. Frontiers Media S.A. 2019-03-28 /pmc/articles/PMC6447708/ /pubmed/30984125 http://dx.doi.org/10.3389/fmicb.2019.00562 Text en Copyright © 2019 Ge, Domesle, Yang, Hammack, Wang, Deng, Hu, Zhang, Hu, Lai, Chou, Dollete, Hirneisen, La, Richter, Rai, Yousefvand, Park, Wu, Eames, Kiang, Sheng, Wu, Hahn, Ledger, Logie, You, Slavic, Cai, Ayers, Young and Pamboukian. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Ge, Beilei Domesle, Kelly J. Yang, Qianru Hammack, Thomas S. Wang, Shizhen S. Deng, Xiaohong Hu, Lijun Zhang, Guodong Hu, Yuan Lai, Xiaokuang Chou, Kyson X. Dollete, Jan Ryan Hirneisen, Kirsten A. La, Sammie P. Richter, Richelle S. Rai, Diyo R. Yousefvand, Azadeh A. Park, Paul K. Wu, Cindy H. Eames, Tameji Kiang, David Sheng, Ju Wu, Dancia Hahn, Lori Ledger, Lisa Logie, Cynthia You, Qiu Slavic, Durda Cai, Hugh Ayers, Sherry L. Young, Shenia R. Pamboukian, Ruiqing Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food |
title | Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food |
title_full | Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food |
title_fullStr | Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food |
title_full_unstemmed | Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food |
title_short | Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food |
title_sort | multi-laboratory validation of a loop-mediated isothermal amplification method for screening salmonella in animal food |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447708/ https://www.ncbi.nlm.nih.gov/pubmed/30984125 http://dx.doi.org/10.3389/fmicb.2019.00562 |
work_keys_str_mv | AT gebeilei multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT domeslekellyj multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT yangqianru multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT hammackthomass multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT wangshizhens multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT dengxiaohong multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT hulijun multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT zhangguodong multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT huyuan multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT laixiaokuang multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT choukysonx multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT dolletejanryan multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT hirneisenkirstena multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT lasammiep multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT richterrichelles multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT raidiyor multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT yousefvandazadeha multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT parkpaulk multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT wucindyh multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT eamestameji multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT kiangdavid multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT shengju multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT wudancia multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT hahnlori multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT ledgerlisa multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT logiecynthia multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT youqiu multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT slavicdurda multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT caihugh multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT ayerssherryl multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT youngsheniar multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood AT pamboukianruiqing multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood |