Cargando…

Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food

Loop-mediated isothermal amplification (LAMP) has gained wide popularity in the detection of Salmonella in foods owing to its simplicity, rapidity, and robustness. This multi-laboratory validation (MLV) study aimed to validate a Salmonella LAMP-based method against the United States Food and Drug Ad...

Descripción completa

Detalles Bibliográficos
Autores principales: Ge, Beilei, Domesle, Kelly J., Yang, Qianru, Hammack, Thomas S., Wang, Shizhen S., Deng, Xiaohong, Hu, Lijun, Zhang, Guodong, Hu, Yuan, Lai, Xiaokuang, Chou, Kyson X., Dollete, Jan Ryan, Hirneisen, Kirsten A., La, Sammie P., Richter, Richelle S., Rai, Diyo R., Yousefvand, Azadeh A., Park, Paul K., Wu, Cindy H., Eames, Tameji, Kiang, David, Sheng, Ju, Wu, Dancia, Hahn, Lori, Ledger, Lisa, Logie, Cynthia, You, Qiu, Slavic, Durda, Cai, Hugh, Ayers, Sherry L., Young, Shenia R., Pamboukian, Ruiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447708/
https://www.ncbi.nlm.nih.gov/pubmed/30984125
http://dx.doi.org/10.3389/fmicb.2019.00562
_version_ 1783408552928346112
author Ge, Beilei
Domesle, Kelly J.
Yang, Qianru
Hammack, Thomas S.
Wang, Shizhen S.
Deng, Xiaohong
Hu, Lijun
Zhang, Guodong
Hu, Yuan
Lai, Xiaokuang
Chou, Kyson X.
Dollete, Jan Ryan
Hirneisen, Kirsten A.
La, Sammie P.
Richter, Richelle S.
Rai, Diyo R.
Yousefvand, Azadeh A.
Park, Paul K.
Wu, Cindy H.
Eames, Tameji
Kiang, David
Sheng, Ju
Wu, Dancia
Hahn, Lori
Ledger, Lisa
Logie, Cynthia
You, Qiu
Slavic, Durda
Cai, Hugh
Ayers, Sherry L.
Young, Shenia R.
Pamboukian, Ruiqing
author_facet Ge, Beilei
Domesle, Kelly J.
Yang, Qianru
Hammack, Thomas S.
Wang, Shizhen S.
Deng, Xiaohong
Hu, Lijun
Zhang, Guodong
Hu, Yuan
Lai, Xiaokuang
Chou, Kyson X.
Dollete, Jan Ryan
Hirneisen, Kirsten A.
La, Sammie P.
Richter, Richelle S.
Rai, Diyo R.
Yousefvand, Azadeh A.
Park, Paul K.
Wu, Cindy H.
Eames, Tameji
Kiang, David
Sheng, Ju
Wu, Dancia
Hahn, Lori
Ledger, Lisa
Logie, Cynthia
You, Qiu
Slavic, Durda
Cai, Hugh
Ayers, Sherry L.
Young, Shenia R.
Pamboukian, Ruiqing
author_sort Ge, Beilei
collection PubMed
description Loop-mediated isothermal amplification (LAMP) has gained wide popularity in the detection of Salmonella in foods owing to its simplicity, rapidity, and robustness. This multi-laboratory validation (MLV) study aimed to validate a Salmonella LAMP-based method against the United States Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) Chapter 5 Salmonella reference method in a representative animal food matrix (dry dog food). Fourteen independent collaborators from seven laboratories in the United States and Canada participated in the study. Each collaborator received two sets of 24 blind-coded dry dog food samples (eight uninoculated; eight inoculated at a low level, 0.65 MPN/25 g; and eight inoculated at a high level, 3.01 MPN/25 g) and initiated the testing on the same day. The MLV study used an unpaired design where different test portions were analyzed by the LAMP and BAM methods using different preenrichment protocols (buffered peptone water for LAMP and lactose broth for BAM). All LAMP samples were confirmed by culture using the BAM method. BAM samples were also tested by LAMP following lactose broth preenrichment (paired samples). Statistical analysis was carried out by the probability of detection (POD) per AOAC guidelines and by a random intercept logistic regression model. Overall, no significant differences in POD between the Salmonella LAMP and BAM methods were observed with either unpaired or paired samples, indicating the methods were comparable. LAMP testing following preenrichment in buffered peptone water or lactose broth also resulted in insignificant POD differences (P > 0.05). The MLV study strongly supports the utility and applicability of this rapid and reliable LAMP method in routine regulatory screening of Salmonella in animal food.
format Online
Article
Text
id pubmed-6447708
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-64477082019-04-12 Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food Ge, Beilei Domesle, Kelly J. Yang, Qianru Hammack, Thomas S. Wang, Shizhen S. Deng, Xiaohong Hu, Lijun Zhang, Guodong Hu, Yuan Lai, Xiaokuang Chou, Kyson X. Dollete, Jan Ryan Hirneisen, Kirsten A. La, Sammie P. Richter, Richelle S. Rai, Diyo R. Yousefvand, Azadeh A. Park, Paul K. Wu, Cindy H. Eames, Tameji Kiang, David Sheng, Ju Wu, Dancia Hahn, Lori Ledger, Lisa Logie, Cynthia You, Qiu Slavic, Durda Cai, Hugh Ayers, Sherry L. Young, Shenia R. Pamboukian, Ruiqing Front Microbiol Microbiology Loop-mediated isothermal amplification (LAMP) has gained wide popularity in the detection of Salmonella in foods owing to its simplicity, rapidity, and robustness. This multi-laboratory validation (MLV) study aimed to validate a Salmonella LAMP-based method against the United States Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) Chapter 5 Salmonella reference method in a representative animal food matrix (dry dog food). Fourteen independent collaborators from seven laboratories in the United States and Canada participated in the study. Each collaborator received two sets of 24 blind-coded dry dog food samples (eight uninoculated; eight inoculated at a low level, 0.65 MPN/25 g; and eight inoculated at a high level, 3.01 MPN/25 g) and initiated the testing on the same day. The MLV study used an unpaired design where different test portions were analyzed by the LAMP and BAM methods using different preenrichment protocols (buffered peptone water for LAMP and lactose broth for BAM). All LAMP samples were confirmed by culture using the BAM method. BAM samples were also tested by LAMP following lactose broth preenrichment (paired samples). Statistical analysis was carried out by the probability of detection (POD) per AOAC guidelines and by a random intercept logistic regression model. Overall, no significant differences in POD between the Salmonella LAMP and BAM methods were observed with either unpaired or paired samples, indicating the methods were comparable. LAMP testing following preenrichment in buffered peptone water or lactose broth also resulted in insignificant POD differences (P > 0.05). The MLV study strongly supports the utility and applicability of this rapid and reliable LAMP method in routine regulatory screening of Salmonella in animal food. Frontiers Media S.A. 2019-03-28 /pmc/articles/PMC6447708/ /pubmed/30984125 http://dx.doi.org/10.3389/fmicb.2019.00562 Text en Copyright © 2019 Ge, Domesle, Yang, Hammack, Wang, Deng, Hu, Zhang, Hu, Lai, Chou, Dollete, Hirneisen, La, Richter, Rai, Yousefvand, Park, Wu, Eames, Kiang, Sheng, Wu, Hahn, Ledger, Logie, You, Slavic, Cai, Ayers, Young and Pamboukian. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ge, Beilei
Domesle, Kelly J.
Yang, Qianru
Hammack, Thomas S.
Wang, Shizhen S.
Deng, Xiaohong
Hu, Lijun
Zhang, Guodong
Hu, Yuan
Lai, Xiaokuang
Chou, Kyson X.
Dollete, Jan Ryan
Hirneisen, Kirsten A.
La, Sammie P.
Richter, Richelle S.
Rai, Diyo R.
Yousefvand, Azadeh A.
Park, Paul K.
Wu, Cindy H.
Eames, Tameji
Kiang, David
Sheng, Ju
Wu, Dancia
Hahn, Lori
Ledger, Lisa
Logie, Cynthia
You, Qiu
Slavic, Durda
Cai, Hugh
Ayers, Sherry L.
Young, Shenia R.
Pamboukian, Ruiqing
Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food
title Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food
title_full Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food
title_fullStr Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food
title_full_unstemmed Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food
title_short Multi-Laboratory Validation of a Loop-Mediated Isothermal Amplification Method for Screening Salmonella in Animal Food
title_sort multi-laboratory validation of a loop-mediated isothermal amplification method for screening salmonella in animal food
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447708/
https://www.ncbi.nlm.nih.gov/pubmed/30984125
http://dx.doi.org/10.3389/fmicb.2019.00562
work_keys_str_mv AT gebeilei multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT domeslekellyj multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT yangqianru multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT hammackthomass multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT wangshizhens multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT dengxiaohong multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT hulijun multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT zhangguodong multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT huyuan multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT laixiaokuang multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT choukysonx multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT dolletejanryan multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT hirneisenkirstena multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT lasammiep multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT richterrichelles multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT raidiyor multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT yousefvandazadeha multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT parkpaulk multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT wucindyh multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT eamestameji multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT kiangdavid multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT shengju multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT wudancia multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT hahnlori multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT ledgerlisa multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT logiecynthia multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT youqiu multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT slavicdurda multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT caihugh multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT ayerssherryl multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT youngsheniar multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood
AT pamboukianruiqing multilaboratoryvalidationofaloopmediatedisothermalamplificationmethodforscreeningsalmonellainanimalfood