The investigation of type and concentration of bio-aerosols in the air of surgical rooms: A case study in Shariati hospital, Karaj

The presence of bio-aerosols is one of the main causes of hospital infections that can be dangerous especially for immunocompromised patients. This research aimed to determine the relationship between hospital infections and surgical incision size in addition to determining the concentration and bacterial and fungal bioaerosols found in the operating rooms. This cross-sectional descriptive-analytic study was carried out in the operating rooms of Shariati Hospital in Karaj, Iran during the years 2016 and 2017. A total of 198 bacterial samples and 198 fungal samples were collected and analyzed using a passive sampling standard method (1/1/1) for 180 days. Tryptic Soy Agar (TSA) and Sabouraud Dextrose Agar (SDA) medium were used for bacterial and fungal samples, respectively. Relevant differential tests were used to determine the genus and species of bacteria and fungi such as DNase test, Bile-esculin, motility test urease test. In general, this work presents: • The present evaluated the relationship between bioaerosols concentration and surgical incision size. • The intraoperative concentration of bacterial and fungal bioaerosols in indoor air of the orthopedic, internal and cesarean operating rooms was significantly higher than their preoperative concentration (p-value<0.05). • Also, there was not significant difference between the bacterial and fungal concentrations in various operating rooms) p-value<0.05).