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Controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model

Diabetic foot ulcers (DFU), which may lead to lower extremity amputation, is one of the severe and chronic complications of diabetic mellitus. This study aims to develop, and use dressings based on Silk fibroin (SF) as the scaffold material, gelatin microspheres (GMs) as the carrier for the neuroten...

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Detalles Bibliográficos
Autores principales: Liu, Jianghui, Yan, Liwei, Yang, Wei, Lan, Yong, Zhu, Qiyu, Xu, Hongjie, Zheng, Canbin, Guo, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447858/
https://www.ncbi.nlm.nih.gov/pubmed/30989151
http://dx.doi.org/10.1016/j.bioactmat.2019.03.001
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author Liu, Jianghui
Yan, Liwei
Yang, Wei
Lan, Yong
Zhu, Qiyu
Xu, Hongjie
Zheng, Canbin
Guo, Rui
author_facet Liu, Jianghui
Yan, Liwei
Yang, Wei
Lan, Yong
Zhu, Qiyu
Xu, Hongjie
Zheng, Canbin
Guo, Rui
author_sort Liu, Jianghui
collection PubMed
description Diabetic foot ulcers (DFU), which may lead to lower extremity amputation, is one of the severe and chronic complications of diabetic mellitus. This study aims to develop, and use dressings based on Silk fibroin (SF) as the scaffold material, gelatin microspheres (GMs) as the carrier for the neurotensin (NT), a neuropeptide that acts as an inflammatory modulator in wound healing and NT as accelerate wound healing drug to treat DFU. We evaluated the wound healing processes and neo-tissue formation in rat diabetic model by macroscopic observation, histological observation (H&E staining and Masson's trichrome staining) and immunofluorescence analysis at 3, 7, 14, 21 and 28 post-operation days. Our results show that the NT/GMs/SF group performance the best not only in macroscopic healing and less scars in 28 post-operation days, but also in fibroblast accumulation in tissue granulation, collagen expression and deposition at the wound site. From release profiles, we can know the GMs are a good carrier for control release drugs. The SEM results shows that the NT/GMs/SF dressings have an average pore size are 40–80 μm and a porosity of ∼85%, this pore size is suit for wound healing regeneration. These results suggest that the NT/GMs/SF dressings may work as an effective support for control release NT to promote DFU wound healing.
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spelling pubmed-64478582019-04-15 Controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model Liu, Jianghui Yan, Liwei Yang, Wei Lan, Yong Zhu, Qiyu Xu, Hongjie Zheng, Canbin Guo, Rui Bioact Mater Article Diabetic foot ulcers (DFU), which may lead to lower extremity amputation, is one of the severe and chronic complications of diabetic mellitus. This study aims to develop, and use dressings based on Silk fibroin (SF) as the scaffold material, gelatin microspheres (GMs) as the carrier for the neurotensin (NT), a neuropeptide that acts as an inflammatory modulator in wound healing and NT as accelerate wound healing drug to treat DFU. We evaluated the wound healing processes and neo-tissue formation in rat diabetic model by macroscopic observation, histological observation (H&E staining and Masson's trichrome staining) and immunofluorescence analysis at 3, 7, 14, 21 and 28 post-operation days. Our results show that the NT/GMs/SF group performance the best not only in macroscopic healing and less scars in 28 post-operation days, but also in fibroblast accumulation in tissue granulation, collagen expression and deposition at the wound site. From release profiles, we can know the GMs are a good carrier for control release drugs. The SEM results shows that the NT/GMs/SF dressings have an average pore size are 40–80 μm and a porosity of ∼85%, this pore size is suit for wound healing regeneration. These results suggest that the NT/GMs/SF dressings may work as an effective support for control release NT to promote DFU wound healing. KeAi Publishing 2019-03-28 /pmc/articles/PMC6447858/ /pubmed/30989151 http://dx.doi.org/10.1016/j.bioactmat.2019.03.001 Text en ©E staining and Masson's trichrome staining) and immunofluorescence. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Liu, Jianghui
Yan, Liwei
Yang, Wei
Lan, Yong
Zhu, Qiyu
Xu, Hongjie
Zheng, Canbin
Guo, Rui
Controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model
title Controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model
title_full Controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model
title_fullStr Controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model
title_full_unstemmed Controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model
title_short Controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model
title_sort controlled-release neurotensin-loaded silk fibroin dressings improve wound healing in diabetic rat model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447858/
https://www.ncbi.nlm.nih.gov/pubmed/30989151
http://dx.doi.org/10.1016/j.bioactmat.2019.03.001
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