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A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies
The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop,...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
International Union of Crystallography
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448683/ https://www.ncbi.nlm.nih.gov/pubmed/30996717 http://dx.doi.org/10.1107/S1600576719001900 |
| _version_ | 1783408722256592896 |
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| author | Sharma, Amit Berntsen, Peter Harimoorthy, Rajiv Appio, Roberto Sjöhamn, Jennie Järvå, Michael Björling, Alexander Hammarin, Greger Westenhoff, Sebastian Brändén, Gisela Neutze, Richard |
| author_facet | Sharma, Amit Berntsen, Peter Harimoorthy, Rajiv Appio, Roberto Sjöhamn, Jennie Järvå, Michael Björling, Alexander Hammarin, Greger Westenhoff, Sebastian Brändén, Gisela Neutze, Richard |
| author_sort | Sharma, Amit |
| collection | PubMed |
| description | The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aquaporin was also demonstrated using caged calcium. |
| format | Online Article Text |
| id | pubmed-6448683 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | International Union of Crystallography |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-64486832019-04-17 A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies Sharma, Amit Berntsen, Peter Harimoorthy, Rajiv Appio, Roberto Sjöhamn, Jennie Järvå, Michael Björling, Alexander Hammarin, Greger Westenhoff, Sebastian Brändén, Gisela Neutze, Richard J Appl Crystallogr Research Papers The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aquaporin was also demonstrated using caged calcium. International Union of Crystallography 2019-03-28 /pmc/articles/PMC6448683/ /pubmed/30996717 http://dx.doi.org/10.1107/S1600576719001900 Text en © Amit Sharma et al. 2019 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Research Papers Sharma, Amit Berntsen, Peter Harimoorthy, Rajiv Appio, Roberto Sjöhamn, Jennie Järvå, Michael Björling, Alexander Hammarin, Greger Westenhoff, Sebastian Brändén, Gisela Neutze, Richard A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies |
| title | A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies |
| title_full | A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies |
| title_fullStr | A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies |
| title_full_unstemmed | A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies |
| title_short | A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies |
| title_sort | simple adaptation to a protein crystallography station to facilitate difference x-ray scattering studies |
| topic | Research Papers |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448683/ https://www.ncbi.nlm.nih.gov/pubmed/30996717 http://dx.doi.org/10.1107/S1600576719001900 |
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