Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro

Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus diseases and porcine circovirus-associated diseases, which are widespread in swine-producing countries. However, there is controversy regarding the susceptibility of human cells to PCV2 infection. In this study, human cell lines...

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Autores principales: Liu, Xiaohui, Ouyang, Ting, Ouyang, Hongsheng, Liu, Xiaohua, Niu, Guyu, Huo, Wang, Yin, Weihong, Pang, Daxin, Ren, Linzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449348/
https://www.ncbi.nlm.nih.gov/pubmed/30948762
http://dx.doi.org/10.1038/s41598-019-42210-0
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author Liu, Xiaohui
Ouyang, Ting
Ouyang, Hongsheng
Liu, Xiaohua
Niu, Guyu
Huo, Wang
Yin, Weihong
Pang, Daxin
Ren, Linzhu
author_facet Liu, Xiaohui
Ouyang, Ting
Ouyang, Hongsheng
Liu, Xiaohua
Niu, Guyu
Huo, Wang
Yin, Weihong
Pang, Daxin
Ren, Linzhu
author_sort Liu, Xiaohui
collection PubMed
description Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus diseases and porcine circovirus-associated diseases, which are widespread in swine-producing countries. However, there is controversy regarding the susceptibility of human cells to PCV2 infection. In this study, human cell lines were infected with PCV2 and blind passaged several times. PCV2 entered and replicated in human cells, and infectious virions were generated, indicating that human cell lines were permissive to PCV2 replication. Furthermore, PCV2 replication in human cell lines was enhanced by D-glucosamine or concanavalin A (ConA). However, the infection efficiency of PCV2 was lower in human cells than in PK-15 cells, suggesting that PCV2 infection was limited in human cells. Our study reveals that human cells are permissive for the productive infection of porcine circovirus type 2 in vitro.
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spelling pubmed-64493482019-04-10 Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro Liu, Xiaohui Ouyang, Ting Ouyang, Hongsheng Liu, Xiaohua Niu, Guyu Huo, Wang Yin, Weihong Pang, Daxin Ren, Linzhu Sci Rep Article Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus diseases and porcine circovirus-associated diseases, which are widespread in swine-producing countries. However, there is controversy regarding the susceptibility of human cells to PCV2 infection. In this study, human cell lines were infected with PCV2 and blind passaged several times. PCV2 entered and replicated in human cells, and infectious virions were generated, indicating that human cell lines were permissive to PCV2 replication. Furthermore, PCV2 replication in human cell lines was enhanced by D-glucosamine or concanavalin A (ConA). However, the infection efficiency of PCV2 was lower in human cells than in PK-15 cells, suggesting that PCV2 infection was limited in human cells. Our study reveals that human cells are permissive for the productive infection of porcine circovirus type 2 in vitro. Nature Publishing Group UK 2019-04-04 /pmc/articles/PMC6449348/ /pubmed/30948762 http://dx.doi.org/10.1038/s41598-019-42210-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, Xiaohui
Ouyang, Ting
Ouyang, Hongsheng
Liu, Xiaohua
Niu, Guyu
Huo, Wang
Yin, Weihong
Pang, Daxin
Ren, Linzhu
Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro
title Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro
title_full Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro
title_fullStr Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro
title_full_unstemmed Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro
title_short Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro
title_sort human cells are permissive for the productive infection of porcine circovirus type 2 in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449348/
https://www.ncbi.nlm.nih.gov/pubmed/30948762
http://dx.doi.org/10.1038/s41598-019-42210-0
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